Human procollagen type III ,HPCⅢ ELISA kit

Code CSB-E11989h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
procollagen type III ,HPCⅢ
Alternative Names
procollagen type 3 ,HPC3,procollagen type III ,HPCⅢ
Homo sapiens (Human)
Sample Types
Detection Range
25 ng/mL-300 ng/mL
25 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human HPCⅢ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-92  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 93  
Range % 90-96  
1:8 Average % 95  
Range % 89-99  
The recovery of human HPCⅢ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-101  
EDTA plasma (n=4) 97 92-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
300 1.356 1.342 1.349    
200 0.967 1.023 0.995    
100 0.466 0.482 0.474    
50 0.211 0.199 0.205    
25 0.050 0.048 0.049    
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days

This Human HPCⅢ ELISA Kit was designed for the quantitative measurement of Human HPCⅢ protein in serum. It is a Sandwich ELISA kit, its detection range is 25 ng/mL-300 ng/mL and the sensitivity is 25 ng/mL.

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