Mouse Beta-Endorphin,β-EP ELISA Kit

Code CSB-E06827m
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse β-EP ELISA Kit was designed for the quantitative measurement of Mouse β-EP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL .

Target Name Beta-Endorphin,β-EP
Abbreviation β-EP
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 pg/mL-50 pg/mL
Sensitivity 0.195 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-92  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 101  
Range % 97-104  
1:8 Average % 92  
Range % 89-95  
Recovery
The recovery of mouse β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-98  
EDTA plasma (n=4) 94 90-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
50 2.284 2.298 2.291 2.099  
25 1.782 1.788 1.785 1.593  
12.5 1.141 1.169 1.155 0.963  
6.25 0.638 0.601 0.620 0.428  
3.12 0.459 0.436 0.448 0.256  
1.56 0.369 0.342 0.356 0.164  
0.78 0.298 0.284 0.291 0.099  
0 0.199 0.185 0.192    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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