Rat Beta-Endorphin,β-EP ELISA Kit

Code CSB-E08206r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Beta-Endorphin,β-EP
Abbreviation
β-EP
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
10 pg/mL-2000 pg/mL
Sensitivity
5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-90  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 94  
Range % 90-98  
1:8 Average % 92  
Range % 88-96  
Recovery
The recovery of rat β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 90 84-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.214 0.212 0.213    
500 0.365 0.336 0.351    
160 0.534 0.578 0.556    
40 0.939 0.994 0.967    
10 1.424 1.476 1.450    
0 2.381 2.259 2.320    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat β-EP ELISA Kit was designed for the quantitative measurement of Rat β-EP protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 pg/mL-2000 pg/mL and the sensitivity is 5 pg/mL.

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