Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.
Research Area
Microbiology
Alternative Names
aqpZ; bniP; b0875; JW0859; Aquaporin Z; Bacterial nodulin-like intrinsic protein
Species
Escherichia coli (strain K12)
Source
in vitro E.coli expression system
Expression Region
1-231aa
Target Protein Sequence
MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Buffer
Tris-based buffer，50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active) is a full length protein expressed with N-terminal 6xHis-tagged in vitro E.coli expression system. Its expression region corresponds to 1-231aa of E.coli aqpZ protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band around 25 kDa on the gel. It was validated to be biologically active through functional ELISA analyses. This recombinant aqpZ protein may be used to synthesize antibodies against aqpZ or on the studies of aqpZ-associated signal transduction. This recombinant aqpZ proteins in-stock are available.
aqpZ is a channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Evidence shows that induction of an immune response against AqpZ can trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system.
