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Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active)

Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active)

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Code	CSB-CF352724ENV
Abbreviation	Recombinant E.coli AQPZ protein (Active)
MSDS	MSDS Datasheet
Size	$1620
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC₅₀ of E.coli ytfE protein is 197.90-259.70 μg/ml.
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.

Target Names

aqpZ

Uniprot No.

P60844

Research Area

Microbiology

Alternative Names

aqpZ; bniP; b0875; JW0859; Aquaporin Z; Bacterial nodulin-like intrinsic protein

Species

Escherichia coli (strain K12)

Source

in vitro E.coli expression system

Expression Region

1-231aa

Target Protein Sequence

MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD

Mol. Weight

27.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Aquaporin Z serves as a critical water channel in *E. coli*, and this full-length recombinant form (aa 1–231) retains confirmed binding activity, with functional ELISA demonstrating interaction with YtfE at an EC50 of 197.90–259.70 μg/ml when immobilized at 5 μg/ml. This validated protein–protein interaction supports use in ligand-binding assays, membrane transporter functional studies, and ELISA standard development where confirmed conformational integrity is essential. The N-terminal 6xHis tag facilitates oriented immobilization for surface plasmon resonance or pull-down experiments, enabling drug-protein binding studies and structural or biophysical characterization of channel architecture. Produced using an in vitro *E. coli* expression system, this preparation achieves greater than 90% purity by SDS-PAGE, providing a suitable basis for antibody development campaigns and quantitative binding assays that demand minimal background interference.

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Target Background

Function

Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.

Gene References into Functions

Induction of an immune response against AqpZ or its homologous regions can also trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. PMID: 23008451
Data show that the open and closed states of AqpZ directly relate to the side chain conformation of residue R189. PMID: 21315687
the 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate PMID: 14691544
the difference in Arg-189 displacements is correlated with a strong electron density between first transmembrane helices of two open channels; the observed Arg-189 conformations are stabilized by asymmetrical subunit interactions in tAqpZ PMID: 16239219
analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities PMID: 16399837
biochemical analysis of permeability and solute transport characteristics of amphiphilic triblock-polymer vesicles containing the bacterial water-channel protein Aquaporin Z PMID: 18077364