Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active)

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Code	CSB-CF352724ENV
Size	US$3276Purchase it in Cusabio online store (only available for customers from the US)
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind human ytfE, the EC₅₀ of human ytfE protein is 197.90-259.70 μg/ml.
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Product Details
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Target Data

Product Details

Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind human ytfE, the EC50 of human ytfE protein is 197.90-259.70 μg/ml.
Target Names	aqpz
Uniprot No.	P60844
Research Area	Microbiology
Alternative Names	aqpZ; bniP; b0875; JW0859Aquaporin Z; Bacterial nodulin-like intrinsic protein
Species	Escherichia coli (strain K12)
Source	in vitro E.coli expression system
Expression Region	1-231aa
Target Protein Sequence	MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD
Mol. Weight	27.7kDa
Protein Length	Full Length
Tag Info	N-terminal 6xHis-tagged
Form	Lyophilized powder
Buffer	Lyophilized from 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% Trehalose, pH 8.0
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Data

Function	Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
Gene References into Functions	Induction of an immune response against AqpZ or its homologous regions can also trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. PMID: 23008451 Data show that the open and closed states of AqpZ directly relate to the side chain conformation of residue R189. PMID: 21315687 the 2.5 A resolution structure of AqpZ suggests aquaporin selectivity results both from a steric mechanism due to pore size and from specific amino acid substitutions that regulate the preference for a hydrophobic or hydrophilic substrate PMID: 14691544 the difference in Arg-189 displacements is correlated with a strong electron density between first transmembrane helices of two open channels; the observed Arg-189 conformations are stabilized by asymmetrical subunit interactions in tAqpZ PMID: 16239219 analysis of of Escherichia coli aquaporin AqpZ and GlpF single-channel water permeabilities PMID: 16399837 biochemical analysis of permeability and solute transport characteristics of amphiphilic triblock-polymer vesicles containing the bacterial water-channel protein Aquaporin Z PMID: 18077364 Show More Hide All
Subcellular Location	Cell inner membrane, Multi-pass membrane protein
Protein Families	MIP/aquaporin (TC 1.A.8) family
Database Links	KEGG: ecj:JW0859 STRING: 316385.ECDH10B_0945