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| Code | CSB-CF352724ENV |
| Size | US$3276 How to order? |
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| Description |
Recombinant Escherichia coli Aquaporin Z (aqpZ) (Active) is a full length protein expressed with N-terminal 6xHis-tagged in vitro E.coli expression system. Its expression region corresponds to 1-231aa of E.coli aqpZ protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band around 25 kDa on the gel. It was validated to be biologically active through functional ELISA analyses. This recombinant aqpZ protein may be used to synthesize antibodies against aqpZ or on the studies of aqpZ-associated signal transduction. This recombinant aqpZ proteins in-stock are available.
aqpZ is a channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity. Evidence shows that induction of an immune response against AqpZ can trigger an autoimmune reaction against human Aqp4 and inflammation of the central nervous system. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Activity | Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml. |
| Target Names | aqpZ |
| Uniprot No. | P60844 |
| Research Area | Microbiology |
| Alternative Names | aqpZ; bniP; b0875; JW0859; Aquaporin Z; Bacterial nodulin-like intrinsic protein |
| Species | Escherichia coli (strain K12) |
| Source | in vitro E.coli expression system |
| Expression Region | 1-231aa |
| Target Protein Sequence | MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD |
| Mol. Weight | 27.7kDa |
| Protein Length | Full Length |
| Tag Info |
N-terminal 6xHis-tagged |
| Form | Liquid or Lyophilized powder |
| Buffer | Tris-based buffer，50% glycerol |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Channel that permits osmotically driven movement of water in both directions. It is involved in the osmoregulation and in the maintenance of cell turgor during volume expansion in rapidly growing cells. It mediates rapid entry or exit of water in response to abrupt changes in osmolarity.
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| Gene References into Functions |
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| Subcellular Location | Cell inner membrane; Multi-pass membrane protein. |
| Protein Families | MIP/aquaporin (TC 1.A.8) family |
| Database Links |
KEGG: ecj:JW0859 STRING: 316385.ECDH10B_0945 |
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