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The recombinant Aquaporin-1/AQP1 was produced as a fusion protein with N-terminal 6xHis-SUMO tagged in vitro E.coli expression system. The encoding sequence contains 2-269aa of human AQP1 protein. This full-length of mature AQP1 protein reached up to 90% in purity, which is determined by SDS-PAGE. It migrated to a molecular mass band of 45 kDa on the gel. The slightly higher result is attributed to glycosylation. And it was also verified with a high activity assessed by its binding ability in a functional ELISA. This recombinant AQP1 protein may be used to synthesize anti-AQP1 antibodies or in the studies of AQP1-mediated signal transduction.
AQP1 is one of the aquaporins, water channels that are critical to regulating water fluid homeostasis in cells involved in inflammation. It exerts a role in macrophage migration and AQP1-mediated some phenotypic and migratory modifications of these cells are crucial for the switch of M0/M2 phenotype. AQP1 localized on lung capillaries may participate in the development of angiogenesis. Particularly, the elimination of AQP1 reduces endothelial cell migration, limiting tumor angiogenesis, and growth. It has been demonstrated that AQP1-expressing tumor cells augment local infiltration and metastatic effects.
|Purity||Greater than 90% as determined by SDS-PAGE.|
|Research Area||Signal Transduction|
AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
|Species||Homo sapiens (Human)|
|Source||in vitro E.coli expression system|
|Target Protein Sequence||ASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Mol. Weight||41.3 kDa
|Protein Length||Full Length of Mature Protein|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer||Tris-based buffer，50% glycerol|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time|| Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
|Gene References into Functions||
|Subcellular Location||Cell membrane; Multi-pass membrane protein.|
|Protein Families||MIP/aquaporin (TC 1.A.8) family|
|Tissue Specificity||Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.|