Recombinant Human Aquaporin-1(AQP1) (Active)

Code CSB-CF001957HU(A4)
Size US$3335Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Activity Measured by its binding ability in a functional ELISA. Immobilized AQP1 at 2 μg/ml can bind human RGS17, the EC50 of human RGS17 protein is 31.63-34.44 μg/ml.
Target Names AQP1
Uniprot No. P29972
Research Area Signal Transduction
Alternative Names AQP 1; AQP CHIP; AQP-1; AQP1; AQP1_HUMAN; aquaporin 1 (channel-forming integral protein; 28kDa; CO blood group); aquaporin 1 (Colton blood group); Aquaporin CHIP; Aquaporin-1; Aquaporin-CHIP; Aquaporin1; Channel forming integral protein 28kDa; Channel like integral membrane protein 28 kDa; CHIP 28; CHIP28; CO; Colton blood group; Growth factor induced delayed early response protein; MGC26324; Urine water channel; Water channel protein CHIP 29; Water channel protein CHIP29; Water channel protein for red blood cells and kidney proximal tubule
Species Homo sapiens (Human)
Source in vitro E.coli expression system
Expression Region 2-269aa
Target Protein Sequence ASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK
Mol. Weight 44.4 kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Gene References into Functions
  1. Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in heritable pulmonary arterial hypertension. PMID: 29650961
  2. Study demonstrated that a region upstream of the AQP1 gene (- 2300 to - 2200 bp) contains an enhancer required for pH-mediated regulation of AQP1 gene expression. PMID: 29554889
  3. H19 acted as AQP1 ceRNA in regulating miR-874. PMID: 30021355
  4. Data show that miR-320 negatively regulates aquaporin 1 (AQP1) expression by targeting its 3' untranslated regions (3'-UTR). PMID: 29538612
  5. The present study represents further confirmation of the hypothesized prognostic role of AQP1, which seems a reliable prognostic indicator. PMID: 29495596
  6. AQP1 may serve a role in glioblastoma multiforme migration, invasion and vasculogenic mimicry formation PMID: 29257313
  7. Adenovirus-mediated human AQP1 expression in hepatocytes improves lipopolysaccharide-induced cholestasis in rats. PMID: 29087027
  8. AQP1 may interact with VEGFA and play a role in vasculogenic mimicry, especially under hypoxic conditions, but the heterogeneity of malignant mesothelioma cells may result in different dominant pathways between patients. PMID: 29104239
  9. AQP1, 3 and 5 expression through the stages of human salivary gland morphogenesis, was examined. PMID: 28766180
  10. AQP1 was elevated in the majority of ovarian cancer tissues. Notably, the present study, to the best of our knowledge, suggests for the first time that AQP1 exhibits a key role in the viability, apoptosis, migration and invasion of ovarian cancer cells. PMID: 28849036
  11. AQP1 is expressed in atherosclerotic lesion neovasculature in human and mouse arteries and AQP1 deficiency augments lesion development in angiotensin II-promoted atherosclerosis in mice. PMID: 28129470
  12. Strong aquaporin 1 protein expression was an independent adverse prognosticator in hilar cholangiocarcinoma. PMID: 27143047
  13. Findings suggest that a defined population of AQP4- and AQP1-expressing reactive astrocytes may modify alpha-syn deposition in the neocortex of patients with Parkinson's Disease. PMID: 26919570
  14. The BRAF V600 mutations were significantly associated with AQP1 expression (P=0.014). Long-term follow-up indicated a reduced progression-free survival (P=0.036) and overall survival (P=0.017) for the AQP1-positive cutaneous melanoma patients. PMID: 26848795
  15. The expression of AQP1 and 3 is a manifestation of physiological adaptation of functionally mature chondrocytes able to respond to the change of their internal environment influenced by extracellular matrix. PMID: 28708257
  16. function of AQP1 in tonicity response could be coupled or correlated to its function in band 3-mediated CO2/HCO3(-) exchange PMID: 28596233
  17. Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels. PMID: 27835672
  18. The native AQP1 gene, normally latent in human salivary gland cells, can be activated by both promoter-independent artificial transcription and epigenetic editing of the promoter. PMID: 28072927
  19. data suggest an important functional role of AQP1 in the pathobiology of hypoxia-induced pulmonary hypertension PMID: 28409279
  20. We demonstrated that AQP1-like immunoreactivity and expression are significantly increased in plaques of Peyronie's disease patients Vs controls, implying that AQP1 overexpression might be the consequence of a localized maladaptive response of the connective tissue to repeated mechanical trauma PMID: 26972451
  21. AQP-1 overexpression is associated with prostate cancer progression. PMID: 27817002
  22. Study provides evidence that Mef2c cooperated with Sp1 to activate human AQP1 transcription by binding to its proximal promoter in human umbilical cord vein endothelial cells indicating that AQP1 is a direct target of Mef2c in regulating angiogenesis and vasculogenesis of endothelial cells. PMID: 26923194
  23. AQP1 was overexpressed in cystic cholangiocytes of patients with polycystic liver disease. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. PMID: 26838488
  24. Aquaporin1 overexpression restored anti-cell proliferation and metastatic effect of si-Gli1. PMID: 27157540
  25. AQP1 expression was significantly lower in hereditary spherocytosis patients and silent carriers than in the simultaneously processed normal control, while no AQP1 decrease was detected in the autoimmune hemolytic anemia sample. The decreased AQP1 expression could contribute to explain variable degrees of anemia in hereditary spherocytosis. PMID: 27465156
  26. High cytoplasmic expression of AQP1 is associated with breast cancer progression. PMID: 26812884
  27. Suggest that sputum AQP1 and AQP5 could be used as diagnostic markers in mild-to-moderate adult-onset asthma. PMID: 26823734
  28. the downregulation of AQP1 impedes extracellular to intracellular fluid transportation. PMID: 26549133
  29. Based on a comparative mutagenic analysis of AQP1, AQP 3, and AQP 4, the results suggest that loop D interactions may provide a general structural framework for tetrameric assembly within the AQP family. PMID: 26786101
  30. The role of AQP1 in malignant pleural mesothelioma. PMID: 26773069
  31. Urinary AQP1 is significantly lower in patients with clear cell renal carcinoma than in controls. Urinary AQP1 increases after nephrectomy in clear cell renal carcinoma patients. PMID: 27295912
  32. AQP1 knockdown can effectively inhibit cell proliferation, adhesion, invasion and tumorigenesis by targeting TGF-beta signaling pathway and focal adhesion genes. PMID: 26176849
  33. These data provide support for a continuous role of KLF2 in stabilizing the vessel wall via co-temporal expression of eNOS and AQP1 both preceding and during the pathogenesis of atherosclerosis. PMID: 26717516
  34. The study demonstrated the abnormal expression pattern of AQP1, AQP2, AQP3, and AQP4 in the kidney tissues of patients with nephrotic syndrome, providing a basis for an improved understanding of the role of aquaporins in the pathogenesis of this disease. PMID: 26261083
  35. The results suggest that AQP1 may facilitate lung cancer cell proliferation and migration in an MMP-2 and-9-dependent manner PMID: 26151179
  36. PBEF regulates the expression of inflammatory factors and AQP1 through the MAPK pathways. PMID: 26178576
  37. the benign subependymomas aquaporins 1 and 4 are dramatically redistributed and upregulated. PMID: 26115524
  38. AQP1 was found to be a marker of a subgroup of aggressive basaloid-like squamous cell carcinomas PMID: 26074259
  39. High AQP1 expression is associated with recurrence in bladder uroepithelial cell carcinoma . PMID: 25987071
  40. The AQP1 rs1049305 single nucleotide polymorphism is associated with running performance, but not relative body weight change, during South African Ironman Triathlons. PMID: 25495276
  41. The data suggest that the increased expression of AQP1 and AQP3 in pterygial tissues may be involved in the pathogenesis of pterygia PMID: 23974882
  42. CAII increases water conductance through AQP1 by a physical interaction between the two proteins. PMID: 25609088
  43. Aquaporin 1 and 3 were upregulated in cervical cancer compared to mild cervicitis and cervical intraepithelial neoplasia 2-3 (P<0.05). PMID: 24918928
  44. Through molecular genotyping we also identified polymorphisms in RhCE, Kell, Duffy, Colton, Lutheran and Scianna loci in donors and patients. PMID: 25582271
  45. Study found a significant correlation between AQP1, AQP3, and AQP5 overexpression and lymph node metastasis in patients with surgically resected colon cancer. PMID: 25721378
  46. the reduction of aquaporins 1 and 3 may be a factor resulting in lumbar intervertebral disc degeneration. PMID: 25299207
  47. AQP1 over-expression effectively inhibited cell proliferation and induced cell growth arrest in G1 phase of K562 cells. PMID: 25252847
  48. Aquaporin-1 is induced in leukocytes of patients with sepsis and exhibits higher expression in septic shock. PMID: 24028651
  49. Report is the first to establish astrocytic water channel loss in a subset of human central pontine myelinolysis (CPM) cases and suggests AQP1 and AQP4 may be involved in the pathogenesis of CPM PMID: 24252214
  50. Aquaporin-1 is associated with arterial capillary proliferation and hepatic sinusoidal transformation contributing to portal hypertension in primary biliary cirrhosis. PMID: 23949237
  51. Results show that in AQP1, helix 3 inverts its orientation in the membrane after the initial insertion, whereas this does not occur in the homologous AQP4. PMID: 24777974
  52. Freshly obtained urine samples from renal cell carcinoma patients are positive for AQP1 whereas archived samples and fresh healthy control samples are negative. PMID: 24803718
  53. the expression of AQP1, at both the mRNA and protein levels, in membranes from PVR and ERM. PMID: 24688444
  54. the prevalence of this AQP1 c.601delG allele in the ethnic minority of Romani PMID: 24333057
  55. Anti-AQP1 autoantibodies are present in a subgroup of patients with chronic demyelination in the central nervous sytem and similarities with anti-AQP4-seronegative neuromyelitis optica spectrum disorders. PMID: 24086369
  56. Over expression of aquaporin 1 causes increased intracranial pressure, and downregulation reduces pressure and alleviates the symptomatology and complications of idiopathic intracranial hypertension. PMID: 24169407
  57. AQP1 promoter hypomethylation is common in ACC, and AQP1 tends to be overexpressed in these tumors. Increased AQP1 methylation is associated with improved prognosis. PMID: 24493792
  58. AQP1 and 4 gene expression levels did not differ between mesial temporal sclerosis patients and control groups PMID: 23928039
  59. AQP-1 appears to be involved in the arterial capillary proliferation in the cirrhotic liver. [Review] PMID: 23549977
  60. Cox-regression analyses revealed the AQP1 -783G/C genotype status as an independent prognostic-factor when jointly considering other predictors of survivalin glioblastoma multiforme. PMID: 24014128
  61. AQP1(+) cells immediately beneath the epithelial basement membrane may be stromal niche-like cells that directly interact with N-cad(+) limbal basal epithelial progenitor cells. PMID: 23276695
  62. K562 cells show a significant increase of AQP1 expression after retinoic acid-induced erythroid differentiation. PMID: 23268390
  63. AQP1-immunoexpression had a good correlation with high-grade tumors. PMID: 23361277
  64. These findings suggest that NKCC1 and AQP1 participate in meningioma biology and invasion PMID: 23317544
  65. A new AQP1 null allele identified in a Gypsy woman who developed an anti-CO3 during her first pregnancy. PMID: 22348807
  66. hAQP1 is a constitutively open channel that closes mediated by membrane-tension increments. PMID: 23332061
  67. This study demonstrated that laminar shear stress stimulates the endothelial expression of AQP1 that plays a role in wound healing. PMID: 23220481
  68. Estrogen induces AQP1 expression by activating ERE in the promoter of the Aqp1 gene, resulting in tubulogenesis of vascular endothelial cells. PMID: 23450058
  69. AQP-1 expression is increased in colorectal carcinoma while the expression of AQP-3 is not. There is no correlation between the expression of AQP-1 and AQP-3 in CRC. PMID: 21538271
  70. High AQP1 expression is associated with malignant pleural mesothelioma. PMID: 23313295
  71. AQP1 expression correlates with the grade of malignancy in astrocytoma and is associated with angiogenesis, as well as with invasion of grade IV tumour in areas of tumour infiltration PMID: 23393355
  72. Data indicate reconstitution of AQP-1 into cholesterol-containing vesicles leads to drastic increases in P(CO2). PMID: 22964306
  73. Stomatin interacts with GLUT1/SLC2A1, band 3/SLC4A1, and aquaporin-1 in human erythrocyte membrane domains PMID: 23219802
  74. Selective expression of AQP1 in the trigeminal neurons innervating the oral mucosa indicates an involvement of AQP1 in oral sensory transduction. PMID: 23029502
  75. The expression of AQP-1 mRNA was positively correlated with Bcl-2 mRNA expression in nasal polyps. AQP-1 contributes to the survival of eosinophils in nasal polyps. PMID: 15563082
  76. AQP1 expression significantly increased in advanced stage of cervical cancer, deeper infiltration, metastatic lymph nodes and larger tumor volume. PMID: 22901156
  77. Exosomal AQP1 is downregulated after the release of ureteropelvic junction obstruction. This may be due to locally increased TGF-beta-1 in the postobstructed kidney. PMID: 22901921
  78. AQP1 is normally expressed in the temporomandibular joint (TMJ) disc with a role in the maintenance of TMJ homeostasis PMID: 22269467
  79. novel pathway in mammalian cells whereby a hypotonic stimulus directly induces intracellular calcium elevations through transient receptor potential channels, which trigger AQP1 translocation PMID: 22334691
  80. expression of aquaporin 1 and 5 was higher in uterine leiomyomata than in unaffected uteri PMID: 22472942
  81. regulates microvessel permeability and barrier function and contributes to blood vessel formation PMID: 22310126
  82. Aberrant expressions of AQP1 in periportal sinusoidal regions in human cirrhotic liver indicate the proliferation of arterial capillaries directly connected to the sinusoids, contributing to microvascular resistance in cirrhosis. PMID: 22093331
  83. We found AQP1 and AQP4 in lung cancer cell extravasation and spread, which may provide a functional explanation for the expression of AQP1 and AQP4 in lung cancer tissues and lung cancer cell lines. PMID: 22372348
  84. our results suggest, for the first time, that the rs1049305 (C/G, UTR3) AQP1 polymorphism could be involved in the genetic susceptibility to develop water retention in patients with liver cirrhosis. PMID: 21793635
  85. The mean concentration of AQP1 in CSF was significantly elevated in patients with BM (BM: 3.8+/-3.4ng/ml, controls PMID: 21896312
  86. This study demonistrated that AQP1 in inner ear plays an important role in the development of motion sickmness, and might be a potential target for the prevention or management of motion sickness. PMID: 21839760
  87. Phosphorylation of tyrosine Tyr253 in the carboxyl terminal domain, acts as a master switch regulating responsiveness of AQP1 ion channels to cGMP, and the tetrameric central pore is the ion permeation pathway. PMID: 22006723
  88. REVIEW: summarizes literature data concerning the involvement of AQP-1 and -4 in human brain tumor growth and edema formation PMID: 21784068
  89. AQP1 and VEGF had a higher expression in nasopharyngeal carcinoma tissues than in non-tumor tissues. PMID: 21395179
  90. AQP1 is a promising oncogene candidate for ACC and is transcriptionally regulated by promoter hypomethylation. PMID: 21551254
  91. The expression level of AQP1 of patients with preeclampsia increases in placenta and peritoneum and decreases in embryolemma. PMID: 19080511
  92. Negative stain transmission electron microscopy and single particle analysis of KCC4 and the aquaporin-1 AQP1 water channel, revealed the expected quaternary structures within homogeneous preparations, and thus correct protein folding and assembly. PMID: 21760919
  93. Inhibiting AQP1 expression with siRNA can inhibit the proliferation and induce apoptosis of K562 cells. PMID: 20137115
  94. hypoxia-induced expression of AQP1 requires transcriptional activation, and the HIF-1 binding site of the 5'-promoter is necessary for transcriptional activation PMID: 21612401
  95. Water flux through human aquaporin 1 was studied under osmotic challenges and the inhibition by intracellular furosemide. PMID: 21373963
  96. The researchers found an association between carriers of the AQP1 single nucleotide polymorphism and greater fluid loss in long-distance running. PMID: 21271497
  97. AQP1 and AQP5 might play an important role in the development of lung edema and lung injury PMID: 21244858
  98. Expression patterns of aquaporin 1, 3, and 5 in lung cancer cells are mostly associated with cellular differentiation. PMID: 21237499
  99. The late rise of AQP1 suggests a role in corpus luteum formation. PMID: 21252246
  100. An important role of AQP1 in tumor angiogenesis is sustained by the abundant expression of this protein in the endothelia of tumor capillaries. PMID: 21360438

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Subcellular Location Cell membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Tissue Specificity Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Database Links

HGNC: 633

OMIM: 107776

KEGG: hsa:358

STRING: 9606.ENSP00000311165

UniGene: Hs.76152

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