Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
2-269aa
Target Protein Sequence
ASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Aquaporin-1/AQP1 was produced as a fusion protein with N-terminal 6xHis-SUMO tagged in vitro E.coli expression system. The encoding sequence contains 2-269aa of human AQP1 protein. This full-length of mature AQP1 protein reached up to 90% in purity, which is determined by SDS-PAGE. It migrated to a molecular mass band of 45 kDa on the gel. The slightly higher result is attributed to glycosylation. And it was also verified with a high activity assessed by its binding ability in a functional ELISA. This recombinant AQP1 protein may be used to synthesize anti-AQP1 antibodies or in the studies of AQP1-mediated signal transduction.
AQP1 is one of the aquaporins, water channels that are critical to regulating water fluid homeostasis in cells involved in inflammation. It exerts a role in macrophage migration and AQP1-mediated some phenotypic and migratory modifications of these cells are crucial for the switch of M0/M2 phenotype. AQP1 localized on lung capillaries may participate in the development of angiogenesis. Particularly, the elimination of AQP1 reduces endothelial cell migration, limiting tumor angiogenesis, and growth. It has been demonstrated that AQP1-expressing tumor cells augment local infiltration and metastatic effects.