Name: Recombinant Human Aquaporin-1(AQP1)
Brand: CUSABIO
SKU: CSB-CF001957HU(A4)

Product Details

Description	The recombinant Aquaporin-1/AQP1 was produced as a fusion protein with N-terminal 6xHis-SUMO tagged in vitro E.coli expression system. The encoding sequence contains 2-269aa of human AQP1 protein. This full-length of mature AQP1 protein reached up to 90% in purity, which is determined by SDS-PAGE. It migrated to a molecular mass band of 45 kDa on the gel. The slightly higher result is attributed to glycosylation. And it was also verified with a high activity assessed by its binding ability in a functional ELISA. This recombinant AQP1 protein may be used to synthesize anti-AQP1 antibodies or in the studies of AQP1-mediated signal transduction. AQP1 is one of the aquaporins, water channels that are critical to regulating water fluid homeostasis in cells involved in inflammation. It exerts a role in macrophage migration and AQP1-mediated some phenotypic and migratory modifications of these cells are crucial for the switch of M0/M2 phenotype. AQP1 localized on lung capillaries may participate in the development of angiogenesis. Particularly, the elimination of AQP1 reduces endothelial cell migration, limiting tumor angiogenesis, and growth. It has been demonstrated that AQP1-expressing tumor cells augment local infiltration and metastatic effects.
Purity	Greater than 90% as determined by SDS-PAGE.
Target Names	AQP1
Uniprot No.	P29972
Research Area	Signal Transduction
Alternative Names	AQP1; CHIP28; Aquaporin-1; AQP-1; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule
Species	Homo sapiens (Human)
Source	in vitro E.coli expression system
Expression Region	2-269aa
Target Protein Sequence	ASEFKKKLFWRAVVAEFLATTLFVFISIGSALGFKYPVGNNQTAVQDNVKVSLAFGLSIATLAQSVGHISGAHLNPAVTLGLLLSCQISIFRALMYIIAQCVGAIVATAILSGITSSLTGNSLGRNDLADGVNSGQGLGIEIIGTLQLVLCVLATTDRRRRDLGGSAPLAIGLSVALGHLLAIDYTGCGINPARSFGSAVITHNFSNHWIFWVGPFIGGALAVLIYDFILAPRSSDLTDRVKVWTSGQVEEYDLDADDINSRVEMKPK Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight	41.3 kDa
Protein Length	Full Length of Mature Protein
Tag Info	N-terminal 6xHis-SUMO-tagged
Form	Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer	Tris-based buffer，50% glycerol
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA	Please contact us to get it.

Target Background

Function	Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Gene References into Functions	Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in heritable pulmonary arterial hypertension. PMID: 29650961 Study demonstrated that a region upstream of the AQP1 gene (- 2300 to - 2200 bp) contains an enhancer required for pH-mediated regulation of AQP1 gene expression. PMID: 29554889 H19 acted as AQP1 ceRNA in regulating miR-874. PMID: 30021355 Data show that miR-320 negatively regulates aquaporin 1 (AQP1) expression by targeting its 3' untranslated regions (3'-UTR). PMID: 29538612 The present study represents further confirmation of the hypothesized prognostic role of AQP1, which seems a reliable prognostic indicator. PMID: 29495596 AQP1 may serve a role in glioblastoma multiforme migration, invasion and vasculogenic mimicry formation PMID: 29257313 Adenovirus-mediated human AQP1 expression in hepatocytes improves lipopolysaccharide-induced cholestasis in rats. PMID: 29087027 AQP1 may interact with VEGFA and play a role in vasculogenic mimicry, especially under hypoxic conditions, but the heterogeneity of malignant mesothelioma cells may result in different dominant pathways between patients. PMID: 29104239 AQP1, 3 and 5 expression through the stages of human salivary gland morphogenesis, was examined. PMID: 28766180 AQP1 was elevated in the majority of ovarian cancer tissues. Notably, the present study, to the best of our knowledge, suggests for the first time that AQP1 exhibits a key role in the viability, apoptosis, migration and invasion of ovarian cancer cells. PMID: 28849036 AQP1 is expressed in atherosclerotic lesion neovasculature in human and mouse arteries and AQP1 deficiency augments lesion development in angiotensin II-promoted atherosclerosis in mice. PMID: 28129470 Strong aquaporin 1 protein expression was an independent adverse prognosticator in hilar cholangiocarcinoma. PMID: 27143047 Findings suggest that a defined population of AQP4- and AQP1-expressing reactive astrocytes may modify alpha-syn deposition in the neocortex of patients with Parkinson's Disease. PMID: 26919570 The BRAF V600 mutations were significantly associated with AQP1 expression (P=0.014). Long-term follow-up indicated a reduced progression-free survival (P=0.036) and overall survival (P=0.017) for the AQP1-positive cutaneous melanoma patients. PMID: 26848795 The expression of AQP1 and 3 is a manifestation of physiological adaptation of functionally mature chondrocytes able to respond to the change of their internal environment influenced by extracellular matrix. PMID: 28708257 function of AQP1 in tonicity response could be coupled or correlated to its function in band 3-mediated CO2/HCO3(-) exchange PMID: 28596233 Data show that the expression of aquaporin (AQP) 1, AQP3, AQP5, epithelial Na+ channel (ENaC) and sodium potassium ATPase (Na-K-ATPase) are altered in patients with acute respiratory failure (ARF) due to diffuse alveolar damage (DAD), and the cause of DAD does not seem to influence the level of impairment of these channels. PMID: 27835672 The native AQP1 gene, normally latent in human salivary gland cells, can be activated by both promoter-independent artificial transcription and epigenetic editing of the promoter. PMID: 28072927 data suggest an important functional role of AQP1 in the pathobiology of hypoxia-induced pulmonary hypertension PMID: 28409279 We demonstrated that AQP1-like immunoreactivity and expression are significantly increased in plaques of Peyronie's disease patients Vs controls, implying that AQP1 overexpression might be the consequence of a localized maladaptive response of the connective tissue to repeated mechanical trauma PMID: 26972451 AQP-1 overexpression is associated with prostate cancer progression. PMID: 27817002 Study provides evidence that Mef2c cooperated with Sp1 to activate human AQP1 transcription by binding to its proximal promoter in human umbilical cord vein endothelial cells indicating that AQP1 is a direct target of Mef2c in regulating angiogenesis and vasculogenesis of endothelial cells. PMID: 26923194 AQP1 was overexpressed in cystic cholangiocytes of patients with polycystic liver disease. A tendency of increased AQP1 protein expression in correlation with the cyst size was also found. PMID: 26838488 Aquaporin1 overexpression restored anti-cell proliferation and metastatic effect of si-Gli1. PMID: 27157540 AQP1 expression was significantly lower in hereditary spherocytosis patients and silent carriers than in the simultaneously processed normal control, while no AQP1 decrease was detected in the autoimmune hemolytic anemia sample. The decreased AQP1 expression could contribute to explain variable degrees of anemia in hereditary spherocytosis. PMID: 27465156 High cytoplasmic expression of AQP1 is associated with breast cancer progression. PMID: 26812884 Suggest that sputum AQP1 and AQP5 could be used as diagnostic markers in mild-to-moderate adult-onset asthma. PMID: 26823734 the downregulation of AQP1 impedes extracellular to intracellular fluid transportation. PMID: 26549133 Based on a comparative mutagenic analysis of AQP1, AQP 3, and AQP 4, the results suggest that loop D interactions may provide a general structural framework for tetrameric assembly within the AQP family. PMID: 26786101 The role of AQP1 in malignant pleural mesothelioma. PMID: 26773069 Urinary AQP1 is significantly lower in patients with clear cell renal carcinoma than in controls. Urinary AQP1 increases after nephrectomy in clear cell renal carcinoma patients. PMID: 27295912 AQP1 knockdown can effectively inhibit cell proliferation, adhesion, invasion and tumorigenesis by targeting TGF-beta signaling pathway and focal adhesion genes. PMID: 26176849 These data provide support for a continuous role of KLF2 in stabilizing the vessel wall via co-temporal expression of eNOS and AQP1 both preceding and during the pathogenesis of atherosclerosis. PMID: 26717516 The study demonstrated the abnormal expression pattern of AQP1, AQP2, AQP3, and AQP4 in the kidney tissues of patients with nephrotic syndrome, providing a basis for an improved understanding of the role of aquaporins in the pathogenesis of this disease. PMID: 26261083 The results suggest that AQP1 may facilitate lung cancer cell proliferation and migration in an MMP-2 and-9-dependent manner PMID: 26151179 PBEF regulates the expression of inflammatory factors and AQP1 through the MAPK pathways. PMID: 26178576 the benign subependymomas aquaporins 1 and 4 are dramatically redistributed and upregulated. PMID: 26115524 AQP1 was found to be a marker of a subgroup of aggressive basaloid-like squamous cell carcinomas PMID: 26074259 High AQP1 expression is associated with recurrence in bladder uroepithelial cell carcinoma . PMID: 25987071 The AQP1 rs1049305 single nucleotide polymorphism is associated with running performance, but not relative body weight change, during South African Ironman Triathlons. PMID: 25495276 The data suggest that the increased expression of AQP1 and AQP3 in pterygial tissues may be involved in the pathogenesis of pterygia PMID: 23974882 CAII increases water conductance through AQP1 by a physical interaction between the two proteins. PMID: 25609088 Aquaporin 1 and 3 were upregulated in cervical cancer compared to mild cervicitis and cervical intraepithelial neoplasia 2-3 (P<0.05). PMID: 24918928 Through molecular genotyping we also identified polymorphisms in RhCE, Kell, Duffy, Colton, Lutheran and Scianna loci in donors and patients. PMID: 25582271 Study found a significant correlation between AQP1, AQP3, and AQP5 overexpression and lymph node metastasis in patients with surgically resected colon cancer. PMID: 25721378 the reduction of aquaporins 1 and 3 may be a factor resulting in lumbar intervertebral disc degeneration. PMID: 25299207 AQP1 over-expression effectively inhibited cell proliferation and induced cell growth arrest in G1 phase of K562 cells. PMID: 25252847 Aquaporin-1 is induced in leukocytes of patients with sepsis and exhibits higher expression in septic shock. PMID: 24028651 Report is the first to establish astrocytic water channel loss in a subset of human central pontine myelinolysis (CPM) cases and suggests AQP1 and AQP4 may be involved in the pathogenesis of CPM PMID: 24252214 Aquaporin-1 is associated with arterial capillary proliferation and hepatic sinusoidal transformation contributing to portal hypertension in primary biliary cirrhosis. PMID: 23949237 Show More Hide All
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	MIP/aquaporin (TC 1.A.8) family
Tissue Specificity	Detected in erythrocytes (at protein level). Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
Database Links	HGNC: 633 OMIM: 107776 KEGG: hsa:358 STRING: 9606.ENSP00000311165 UniGene: Hs.76152

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Code	CSB-CF001957HU(A4)
Size	US$3335
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Aquaporin-1(AQP1)

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