Recombinant Human Indian hedgehog protein(IHH) (Active)

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Code CSB-AP000421HU
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Product Details

Purity >96% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
Target Names IHH
Uniprot No. Q14623
Research Area Stem Cells?
Alternative Names BDA1; HHG-2; HHG2; IHH; IHH_HUMAN; Indian Hedgehog; Indian hedgehog homolog; Indian hedgehog protein; Indian hedgehog protein C-product; Indian hedgehog protein N-product
Species Homo sapiens (Human)
Source E.coli
Expression Region 29-202aa
Complete Sequence II+GPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Mol. Weight 19.8 kDa
Protein Length Partial
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 1 × PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification
Gene References into Functions
  1. TL1A modulated Rheumatoid arthritis-fibroblast-like synoviocytes migration and Indian hedgehog signaling pathway using TNFR2. PMID: 29748156
  2. Association of pathogenic variants in IHH with short stature with nonspecific skeletal abnormalities and a frequent cause of growth disorder, with a preliminary good response to rhGH. PMID: 29155992
  3. Studies suggest significance of other signaling aside from hedgehog in the pathogenesis of basal cell carcinoma (BCC) of the skin. PMID: 28574612
  4. Gorlin syndrome-derived induced pluripotent stem cells (iPSCs) expressed lower basal levels than control iPSCs of the genes encoding the Hh ligands Indian Hedgehog (IHH) and Sonic Hedgehog (SHH). PMID: 29088246
  5. Hedgehog pathway activation in T-cell acute lymphoblastic leukemia predicts response to SMO and GLI1 inhibitors. PMID: 27694322
  6. CLIC4 and Ihh could serve as biological markers for the progression, metastasis and/or invasiveness of pancreatic ductal adenocarcinoma. PMID: 28205343
  7. The serum levels of IHH and SHH were significantly higher in autistic subjects than those of control subjects. The findings support a correlation between SHH, IHH and BDNF in autistic children, suggesting their pathological role in autism. PMID: 26691363
  8. Our results show for the first time that Indian hedgehog does not cause extracellular matrix degradation in healthy ex vivo cartilage or in the presence of IL-1beta PMID: 26705100
  9. The Annexin a2 Promotes Development in Arthritis through Neovascularization by Amplification Hedgehog Pathway. PMID: 26963384
  10. Studies indicate that the hedgehog (Hh) signaling pathway has become one of the most studied potential therapeutic targets in hematological malignancies. PMID: 24991722
  11. Findings demonstrated that Ihh promotes human cartilage endplate degeneration. PMID: 25958162
  12. endogenously produced IHH is playing a critical role in regulating hBMSC chondrogenesis. PMID: 25519748
  13. Duplication of the upstream IHH regulatory region and a correlation between the phenotype and the implicated regulatory regions in a family with craniosynostosis Philadelphia type. PMID: 25692887
  14. Data show that the Hh (Hedgehog) signalling pathway mediates chemoresistance of BCSCs (breast cancer stem cells) and contributes to the outcome in breast cancer patients. PMID: 26201092
  15. Data suggest that hedgehog (HH) signaling through transcription factors GLI1 and GLI2 could be required for the maintenance of pregnancy. PMID: 25888497
  16. Studies indicate that activation of the hedgehog (Hh) signaling pathway has been observed in several cancer types, including ovarian cancer. PMID: 23303278
  17. Gene transfer of indian hedgehog protein results in chondrogenic induction of mesenchymal stem cells. PMID: 22817660
  18. Data indicate that neutralizing hedgehog antibody MEDI-5304 represents a potent dual hedgehog inhibitor suitable for patients with tumors harboring elevated levels of sonic hedgehog (SHH) or indian hedgehog (IHH). PMID: 24344235
  19. Indian Hedgehog, a critical modulator in osteoarthritis, could be a potential therapeutic target for attenuating cartilage degeneration disease. PMID: 24844414
  20. Increased Ihh is associated with the severity of osteoarthritis cartilage damage. Elevated Ihh content in human knee joint synovial fluid correlates with early cartilage lesions. PMID: 24786088
  21. Data indicate that overexpression of Indian hedgehog (Ihh) signaling promotes abnormal chondrocyte differentiation in enchondral ossification and enhances bone formation in cervical ossification of the posterior longitudinal ligament (OPLL). PMID: 23883825
  22. We further indicated that SHH and IHH were highly expressed in metastatic tumors PMID: 23468532
  23. Studies indicate that upregulation of Hedgehog protein has been found in tumor-associated stroma and associated with vascularization and excessive stromal expansion can limit intratumoral drug delivery. PMID: 23575478
  24. Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner. PMID: 23786654
  25. Data indicate that compounds 7 and 9 (diterpene) showed cytotoxicity against cancer cell lines in which hedgehog (Hh) signaling was aberrantly activated. PMID: 23403897
  26. Data indicate that analysis of public microarray data suggests that PBOV1 activation in tumors could be dependent on the Hedgehog signaling pathway. PMID: 23418531
  27. Results suggest that hypoxia-induced increase of Smo directly contributes to the proliferation of pancreatic ductal adenocarcinoma (PDAC) cells through a hedgehog/Gli1-independent pathway. PMID: 22486854
  28. Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen. PMID: 22750747
  29. A missense mutation in IHH gene is observed in an individual affected by brachydactyly type A1. PMID: 22406540
  30. A large duplication involving the IHH locus mimics acrocallosal syndrome PMID: 22234151
  31. Activation of Indian hedgehog promotes chondrocyte hypertrophy and upregulation of MMP-13 in human osteoarthritic cartilage. PMID: 22469853
  32. Ihh-induced Nkx3.2 degradation requires Wnt5a, which is capable of triggering Nkx3.2 degradation PMID: 22507129
  33. Studies indicate that pathways of Hedgehog (Hh), Wnt and Notch, which regulate development during embryonic life and somatic stem cells (SCs) in the adult organism, can be reactivated in malignancies and support tumor-initiating cells (TIC) scompartment. PMID: 22123293
  34. Studies indicate that IHH is required for normal chondrocyte proliferation during both embryonic and postnatal growth. PMID: 21642379
  35. Nuclear and cytoplasmic endometrial expression of Indian hedgehog is decreased in women with endometriosis. PMID: 21640343
  36. VLDL carries Ihh throughout the body in mammals PMID: 20839884
  37. the observed duplications lead to a misexpression and/or overexpression of IHH and by this affect the complex regulatory signaling network during digit and skull development. PMID: 21167467
  38. This data confirms genetic heterogeneity in Brachydactyly A1 (BDA1) and demonstrates that mutations upstream of IHH can result in BDA1. PMID: 20683927
  39. The temporal increase in endometrial IHH during the secretory phase, and their modulation by CDB-2914 suggests progestin role in endometrial differentiation and implantation. PMID: 20881264
  40. down-regulation of indian hedgehog expression as a result of hypermethylation, may be an early event in colorectal carcinogenesis PMID: 20854074
  41. Methylation analysis revealed that IHH promoter was hypermethylated in colon cancer cell lines. PMID: 19856079
  42. Indian hedgehog protein was detected in cystic tubules within human dysplastic kidneys; these molecules modify severity of this congenital malformation. Hedgehog signaling may be important stimulus for renal cyst formation. PMID: 20007344
  43. describes cloning of human IHH PMID: 7720571
  44. Data show that all of the BDA1 mutations occur in a restricted area of the N-terminal active fragment of the IHH. PMID: 19277064
  45. expression is preserved in the growth plate of human fetuses affected with fibroblast growth factor receptor type 3 activating mutations PMID: 12368206
  46. novel mutation causes brachydactyly type A1 PMID: 12384778
  47. A novel missense mutation in the IHH gene mapping to chromosome 5 has been identified identified in all patients of a brachydactyly type A1 family. PMID: 12525541
  48. Homozygous mutations in this gene cause acrocapitofemoral dysplasia, an autosomal recessive disorder with cone-shaped epiphyses in hands and hips. PMID: 12632327
  49. model of the interactions between beta-catenin and hedgehog signaling in the epidermis in which SHH promotes proliferation of progenitors of the hair lineages whereas IHH stimulates proliferation of sebocyte precursors PMID: 12917489
  50. a wide range of digestive tract tumours, including most of those originating in the oesophagus, stomach, biliary tract and pancreas, but not in the colon, display increased Hh pathway activity, which is suppressible by cyclopamine, a Hh pathway antagonist PMID: 14520411
  51. IHH is involved in the development of enteric nervous system but is not a major gene in Hirschsprung's disease. PMID: 14651602
  52. We report that Indian hedgehog (Ihh) is expressed by mature colonocytes and regulates their differentiation in vitro and in vivo. PMID: 14770182
  53. Ihh gene transfer into hTERT stromal cells enhanced their hematopoietic supporting potential in coculture with CD34+ cells PMID: 15105288
  54. aberrant activation of the Ihh signaling pathway contributes to tumor development in pancreatic cancer PMID: 15146555
  55. Hedgehog signaling is conserved in hepatic progenitors from fetal development through adulthood and may be a new therapeutic target in patients with liver damage. PMID: 16322088
  56. DNA sequence analysis revealed a heterozygous C to T transition at nucleotide 461 of IHH, resulting in a novel T154I substitution. The T154I mutation co-segregated with all affected individuals in the family. PMID: 16871364
  57. the subset of MM cells that manifests Hh pathway activity is markedly concentrated within the tumor stem cell compartment PMID: 17360475
  58. Ihh was expressed in 100% of conventional and clear cell chondrosarcomas, especially in matrix-rich areas. Mesenchymal chondrosarcomas revealed only focal expression of Ihh in matrix-rich areas.Ihh was absent or focally expressed in enchondromas. PMID: 17387386
  59. D100N mutation must have arisen independently in these families, suggesting that 298G may be a mutational hot spot. PMID: 17486609
  60. enhanced signaling by Ihh can modulate the proliferation and differentiation of splenic B lymphocytes and thymic T lymphocytes during bone marrow hematopoiesis in vivo PMID: 17962696
  61. A single smino acid deletion in IHH causes mild brachdactyly A1. PMID: 18629882
  62. its signal transduction regulates tumor development. (review) PMID: 18788453
  63. A novel role of Runx2 in up-regulating the vicious cycle of metastatic bone disease, in addition to Runx2 regulation of genes related to progression of tumor metastasis. PMID: 18829534
  64. Hh signaling is important in the pathogenesis of B-CLL and, hence, may be a potential therapeutic target PMID: 19074837
  65. Ihh (Indian hedgehog homolog) signals via the BMP(bone morphogenetic protein) pathway, and specifically through BMP4, to promote endothelial cell differentiation from pluripotent cells PMID: 19213939
  66. An association of paracrine Hedgehog signaling with several gastrointestinal patterning events involving serosa, pylorus, and villus smooth muscle is shown. PMID: 19445942
  67. BDA1 caused by a heterozygous mutation in the IHH gene on chromosome 5 was investigated in a large Swedish family. PMID: 19464397
  68. Mouse study of this member of Hedgehog family of signaling molecules in embryonic patterning and morphogenesis. Detection in target tissues requires proteoglycan/glycosaminoglycan. PMID: 11476578

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Involvement in disease Brachydactyly A1 (BDA1); Acrocapitofemoral dysplasia (ACFD)
Subcellular Location Indian hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space
Protein Families Hedgehog family
Tissue Specificity Expressed in embryonic lung, and in adult kidney and liver.
Database Links

HGNC: 5956

OMIM: 112500

KEGG: hsa:3549

STRING: 9606.ENSP00000295731

UniGene: Hs.654504

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