Product Details

Uniprot No.

Target Names

CENPE

Alternative Names

CENPECentromere-associated protein E antibody; Centromere protein E antibody; CENP-E antibody; Kinesin-7 antibody; Kinesin-related protein CENPE antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthetic peptide of Human CENPE

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized. Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss.

Gene References into Functions

SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores PMID: 27694884
CENP-E motor activity appears to play important roles in the organization of midbody proteins to complete cytokinetic abscission. PMID: 27835888
Data indicate that three genes, KIF14, NCAPG and CENPE that were upregulated in Pediatric high-grade gliomas (pHGGs) and were direct miR-137 or miR-6500-3p targets. PMID: 26933822
CENPE was regulated by the co-binding of LSD1 and AR to its promoter, which was associated with loss of RB1 in CRPC. PMID: 28916652
these results support a novel function of XAB2 in mitotic cell cycle regulation, which is partially mediated by transcription regulation on CENPE. PMID: 27735937
CTCF helps recruit CENP-E to the centromere during mitosis and that it may do so through a structure stabilized by the CTCF/CENP-E complex. PMID: 26321640
Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules. PMID: 25743205
CENP-Q - a subunit of the CENP-O complex (comprising CENP-O, CENP-P, CENP-Q and CENP-U) that targets polo-like kinase (Plk1) to kinetochores - is also required for the recruitment of CENP-E to kinetochores. PMID: 25395579
CENP-E-driven chromosome congression is guided by microtubule detyrosination. PMID: 25908662
CENP-E expression is highest in basal-like subtype among breast cancer patients. PMID: 24928852
An unexpected role of CENP-E elongated stalk in ensuring stability of kinetochore-microtubule attachments during chromosome congression and segregation. PMID: 24920822
dynein and CENP-E at kinetochores drive congression of peripheral polar chromosomes by preventing arm-ejection forces mediated by chromokinesins from working in the wrong direction. PMID: 25383660
Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism. PMID: 24748105
A CENP-E mediated wall-tethering event and a MCAK-mediated wall-removing event show that human chromosome-microtubule attachment is achieved through a set of deterministic sequential events rather than stochastic direct capture of microtubule ends. PMID: 23891108
The changes in ATP binding affinity and conformational deviations in human CENP-E motor domain, were studied. PMID: 23740391
Kinetochore kinesin CENP-E is a processive bi-directional tracker of dynamic microtubule tips. PMID: 23955301
In this study we investigated the pathogenic effect of 132 nsSNPs reported in CENP-E using computational platform. PMID: 22974711
Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. PMID: 23236152
the unusually slow CENP-E microtubule association step favors CENP-E binding of stable microtubules over dynamic ones, a mechanism that would bias CENP-E binding to kinetochore fibers. PMID: 22637578
Data show that CENP-E-mediated traction forces on misaligned chromosomes are responsible for the irreversible loss of spindle-pole integrity in CLASP1/2-depleted cells. PMID: 22307330
SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation. PMID: 22110139
MPS1 is required by cells to arrest in mitosis in response to spindle defects and kinetochore defects resulting from the loss of the kinesin-like protein. PMID: 12686615
Aurora B allowed chromosome alignment in CENP-E-compromised cells; implied that by destabilizing pole proximal syntelic attachments, Aurora B cooperates with CENP-E to mediate congression of mono-oriented polar chromosomes PMID: 20354862
An Aurora/PP1 phosphorylation switch modulates CENP-E motor activity as a feature of chromosome congression from poles and localized PP1 delivery by CENP-E to the outer kinetochore is necessary for stable microtubule capture by those chromosomes. PMID: 20691903
CENP-E expression was reduced in human hepatocellular carcinoma PMID: 20021663
CENP-E integrates two critical functions that are important for accurate chromosome movement and spindle architecture: one relying directly on its motor activity, and the other involving the targeting of key microtubule regulators to kinetochores. PMID: 19733075
x-ray crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure PMID: 15159587
Depletion of CENP-E leads to chromosomes missegregation and cell death during mitotic delay. PMID: 15181147
crystal structure reveals that the CENP-E linker region is in a "docked" position identical to that in the human plus-end directed conventional kinesin PMID: 15236970
Results support a plus end motion for CENP-E, based on a cryoelectron microscopy density map of the complex to 17 A resolution, which is consistent with features of the crystallographic structure. PMID: 16926026
HsNUF2 and CENP-E are required for organization of stable microtubule-kinetochore attachment that is essential for faithful chromosome segregation in mitosis PMID: 17535814
Global inhibition of SUMOylation caused a prometaphase arrest due to defects in targeting the microtubule motor protein CENP-E to kinetochores. PMID: 18374647
Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938
Data show that the kinetochore localization of PinX1 is dependent on Hec1 and CENP-E. PMID: 19553660
Studies strongly suggest that chromosome congression defects as the result of KIF18A depletion is at least in part mediated through destabilizing kinetochore CENP-E. PMID: 19625775

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Involvement in disease

Microcephaly 13, primary, autosomal recessive (MCPH13)

Subcellular Location

Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family

Database Links

HGNC: 1856

OMIM: 117143

KEGG: hsa:1062

STRING: 9606.ENSP00000265148

UniGene: Hs.75573

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Code	CSB-PA963031
Size	US$166
	Order now
Image	The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA963031(CENPE Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA963031(CENPE Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
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CENPE Antibody

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