DIAPH3 Antibody

Code CSB-PA885723LA01HU
Size US$166
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  • Immunofluorescent analysis of HepG2 cells using CSB-PA885723LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DIAPH3 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
AN antibody; AUNA1 antibody; Dia2 antibody; diap3 antibody; DIAP3_HUMAN antibody; DIAPH3 antibody; Diaphanous homolog 3 (Drosophila) antibody; Diaphanous homolog 3 antibody; Diaphanous related formin 3 antibody; Diaphanous; Drosophila; homolog of; 3 antibody; Diaphanous-related formin-3 antibody; DKFZp434C0931 antibody; DKFZp686A13178 antibody; DRF3 antibody; FLJ34705 antibody; mDia2 antibody; NSDAN antibody; OTTHUMP00000018480 antibody; Protein diaphanous homolog 3 antibody; RP11-26P21.1 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Protein diaphanous homolog 3 protein (642-849AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DIAPH3 Antibody (Product code: CSB-PA885723LA01HU) is Non-conjugated. For DIAPH3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA885723LB01HU DIAPH3 Antibody, HRP conjugated ELISA
FITC CSB-PA885723LC01HU DIAPH3 Antibody, FITC conjugated
Biotin CSB-PA885723LD01HU DIAPH3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.
Gene References into Functions
  1. These data identify Diaph3 as a major guard of cortical progenitors, unravel novel functions of Diaphanous formins and add insights into the pathobiology of microcephaly. PMID: 27848932
  2. study demonstrated the oncogenic activity of DIAPH3 in the progression of hepatocellular carcinoma and suggested that PDIAPH3 might be a therapeutic target. PMID: 28795316
  3. Members of 3 generations of a family had dominant auditory neuropathy associated with a c.-172G>A point mutation in DIAPH3. PMID: 27658576
  4. DIAPH3 overexpression inhibits the migration and invasion of Triple-negative breast cancer by inhibiting RhoA-GTP expression. PMID: 28779705
  5. mDia2 and CXCR4 associate in blebs upon CXCL12 stimulation. Both CXCR4 and RhoA are required for CXCL12-induced blebbing PMID: 28115158
  6. we use a simple cellular system to examine fundamental features of formin-mediated filopodial assembly, using constitutively active constructs of the formins mDia2 and FMNL3 PMID: 26446836
  7. results suggest that inhibition of MT stability arising from DIAPH3 downregulation enhances susceptibility to MT poisons, and that the DIAPH3 network potentially reports taxane sensitivity in human tumors PMID: 26179371
  8. Dia1, Dia2, and Dia3 are involved in ErbB2-dependent capture of microtubules at the cell leading edge and ErbB2-driven guided migration. PMID: 24403606
  9. An mDia2/ROCK signaling axis regulates invasive egress from epithelial ovarian cancer spheroids. PMID: 24587343
  10. TGF-beta promotes association of mDia2 with actin stress fibers, which further drives stress fiber formation and myofibroblast differentiation PMID: 23580645
  11. In human tumours, DIAPH3 down-regulation was associated with aggressive or metastatic disease. PMID: 22593025
  12. isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility. PMID: 22184005
  13. the amino terminus of mDia2 serves as a coincidence detection module, directing mDia2 to the plasma membrane through interactions with phospholipids and activated Rif PMID: 21119010
  14. These findings reveal a key role for mDia3 and its regulation by Aurora B phosphorylation in achieving proper stable kinetochore microtubule attachment. PMID: 21397845
  15. These studies further suggest that Rho GTPases significantly influence the activity of mDia family formins in driving cellular membrane remodeling through the regulation of actin dynamics. PMID: 17198702
  16. DIP-induced membrane blebbing was dependent on Diaphanous 2 but did not require the activities of either Diaphanous 1 or Actin-Related Protein 2-3 Complex. PMID: 17398099
  17. Data show that Dia2 localizes in the lamella of migrating epithelial cells, where it is involved in the formation of a stable pool of cortical actin and in maintenance of polymerization-competent free filament barbed ends at focal adhesions. PMID: 17855386
  18. Results suggest that WAVE and the Arp2/3 complex jointly orchestrate different types of actin-based plasma membrane protrusions by promoting ruffling and inhibiting mDia2-induced filopodia. PMID: 18516090
  19. active Drf3 lacking the C-terminal regulatory region (Drf3DeltaDAD) induced the formation of filopodia and accumulated at their tips. PMID: 18755006
  20. mDia2 is degraded at the end of mitosis; mDia2 is targeted for disposal by post-translational ubiquitin modification; forced expression of activated mDia2 yields binucleate cells due to failed cytokinesis PMID: 19457867

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Involvement in disease
Auditory neuropathy, autosomal dominant, 1 (AUNA1)
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Formin homology family, Diaphanous subfamily
Database Links

HGNC: 15480

OMIM: 609129

KEGG: hsa:81624

STRING: 9606.ENSP00000383178

UniGene: Hs.283127

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