HIC1 Antibody

Datasheet
Code CSB-PA623921LA01HU
Size US$299
Image
  • IHC image of CSB-PA623921LA01HU diluted at 1:400 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA623921LA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIC1 Polyclonal antibody
Uniprot No. Q14526
Target Names HIC1
Alternative Names Hic 1 antibody; HIC ZBTB transcriptional repressor 1 antibody; Hic-1 antibody; Hic1 antibody; HIC1_HUMAN antibody; Hypermethylated in cancer 1 antibody; Hypermethylated in cancer 1 protein antibody; ZBTB29 antibody; Zinc finger and BTB domain-containing protein 29 antibody; ZNF901 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Hypermethylated in cancer 1 protein (297-418AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IHC
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF-responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from ACKR3, FGFBP1 and EFNA1.
Gene References into Functions
  1. In this present work, the authors identify and characterize a transcription factor i.e. HIC1, which physically interacts with both Bcl11b/CTIP2 and HMGA1 to co-regulate specific subsets of cellular genes and the HIV-1 tat gene. PMID: 27725726
  2. our data demonstrate that HIC1 SUMOylation is important for the transcriptional response to non-repairable DSBs but dispensable for DNA repair. PMID: 27935866
  3. Data suggest that aberrant expression of hypermethylated in cancer-1 (HIC1)/sirtuin-1 (SIRT1) and hypermethylation of the HIC1 promoter may be critical for the development and progression of papillary thyroid cancer. PMID: 27793057
  4. Hypermethylation of HIC1 gene is associated with Colon Cancer. PMID: 26671036
  5. Data show that secretion of IL-6 induced by loss of HIC1 activated STAT3 through IL-6/JAK pathway and was associated with NSCLC progression. PMID: 27107418
  6. The VNTR sequence near HIC1 could be a predictive marker for oxaliplatin-based chemotherapy in patients with metastatic colorectal cancer. PMID: 28708932
  7. Therefore, our data identify HIC1 as a novel factor involved in B cell differentiation acting as an epigenetic repressor of CIITA transcription. PMID: 27720955
  8. HIC1 loss promotes prostate cancer metastasis by triggering epithelial-mesenchymal transition. PMID: 28466555
  9. Data show that expression of hypermethylated in cancer 1 protein (HIC1) is downregulated in uveal melanoma. PMID: 27449031
  10. HIC1 attenuates invasion and metastasis by inhibiting the IL-6/STAT3 signalling pathway in human pancreatic ductal adenocarcinoma. PMID: 27085461
  11. Epigenetic silencing of HIC1 promotes epithelial-mesenchymal transition and drives progression in esophageal squamous cell carcinoma via EphA2 signaling. PMID: 26510908
  12. The tumor-suppressive function of Hic1 in colon is related to its inhibitory action on proproliferative signaling mediated by the Tlr2 receptor present on tumor cells. PMID: 25934696
  13. Results demonstrated an important role of HIC1 for the normal progression of cell cycle, and could affect the homeostasis of p53 as well as number of cell cycle-related genes, which may or may not be directly linked to p53. PMID: 24992983
  14. We found that EVI1 and HIC1 colocalize in the nucleus, and their interaction is mediated by the amino terminal zinc finger binding domain of EVI1 PMID: 24907396
  15. HIC-1 expression was assessed on a tissue microarray containing 80 cases of breast cancer. PMID: 24489730
  16. Hypermethylation of HIC1 promoter and aberrant expression of HIC1/SIRT1 might contribute to the carcinogenesis of pancreatic cancer. PMID: 22552606
  17. ectopic expression of HIC1 in U2OS and MDA-MB-231 cell lines decreases expression of the ApoER2 and VLDLR genes, encoding two canonical tyrosine kinase receptors for Reelin. PMID: 24076391
  18. HIC1 silencing in triple-negative breast cancer drives progression through misregulation of LCN2. PMID: 24295734
  19. HIC1 interacts with and modulates the transcriptional activity of STAT3. PMID: 24067369
  20. Reactivation of HIC1 suppressed cell migration and induced cell cycle arrest in the G0/G1 phase, as well as induced apoptosis in gastric cancer cells. PMID: 23769968
  21. Our data show for the first time that hypermethylation of HIC1 promoter results in loss of its repressive function, responsible for prostate cancer progression and invasion. PMID: 23340301
  22. epigenetic HIC1 inactivation, which is an early step in tumorigenesis, could contribute to the accumulation of DNA mutations through impaired DNA repair and thus favor tumorigenesis. PMID: 23417673
  23. these results further demonstrate that HIC1 is a key player in the regulation of the DNA damage response. PMID: 23178572
  24. A growth-regulatory role of HIC1 in the parathyroid glands and perturbed expression of HIC1 may represent an early event during tumor development. PMID: 22544915
  25. Promoter hypermethylation of tumor suppressor HIC1 is associated with prostate carcinoma. PMID: 22136354
  26. these results demonstrate that the activating acetylation to SUMOylation switch of HIC1 is favored by genotoxic stresses to regulate the DNA damage response. PMID: 22510409
  27. Identify HIC1 hypermethylation in renal cell carcinoma as an independent predictor of reduced recurrence-free survival. PMID: 22327210
  28. identify HIC1 as the first transcription factor in mammals able to recruit PRC2 to some target promoters through its interaction with Polycomb-like proteins. PMID: 22315224
  29. loss of the regulation of this Eph pathway through HIC1 epigenetic silencing could be an important mechanism in the pathogenesis of epithelial cancers PMID: 22184117
  30. early inactivation of HIC1 in breast carcinomas could predispose to stress-induced metastasis through up-regulation of the beta-2 adrenergic receptor PMID: 22194601
  31. Silencing of HIC1 and TOB1 expression is a common occurrence in gastric cancer and may contribute to the development and progression of the disease. PMID: 21533545
  32. High frequency of methylation at MGMT, RASSFA, and HIC-1 was detected in colorectal carcinoma patients PMID: 21274674
  33. This review highlights the role of HIC1 inactivation in solid tumours and particularly in leukaemia development PMID: 21104471
  34. Maternal genes FLNB, HIC1 and ZNF189 were strongly associated with risk of clefting. PMID: 20634891
  35. Investigated the methylation of the SFRP2, P16, DAPK1, HIC1, and MGMT genes, as well as the mutation of amino acid codons 12 and 13 of the KRAS gene in normal and tumor tissue DNA of patients diagnosed with sporadic colorectal cancer. PMID: 20682398
  36. identify MTA1, a subunit of the NuRD complex, as a new HIC1 corepressor PMID: 20547755
  37. The human candidate tumor suppressor gene HIC1 recruits CtBP through a degenerate GLDLSKK motif. PMID: 12052894
  38. aberrant methylation of HIC1 may play a role in the pathogenesis of specific pediatric tumors PMID: 14506157
  39. epigenetic silencing of HIC-1 may well contribute to the pathogenesis in the majority of medulloblastomas. PMID: 14656076
  40. Inactivation of HIC1 by hypermethylation is associated with medulloblastoma pathogenesis PMID: 14688019
  41. analysis of HIC1 consensus binding sequence and its DNA binding and repressive properties PMID: 15231840
  42. In human osteosarcomas, hypermethylation of HIC1 is frequent only in tumors with p53 mutation PMID: 15488761
  43. HIC1 p53-responsive element (HIC1.PRE) is necessary and sufficient to mediate induction of transcription by p53 PMID: 16301995
  44. HIC1 is involved in a certain feedback regulation for p53 in tumor suppression through histone deacetylase. (review) PMID: 16386221
  45. These data indicate that the intracellular amounts of HIC1 protein can modulate the level of the transcriptional stimulation of the genes regulated by canonical Wnt/beta-catenin signaling. PMID: 16724116
  46. HIC1 interaction with the corepressor CtBP depends on a central leucine residue PMID: 16762039
  47. HIC1 is a target of the class III deacetylase SIRT1 and identify a new posttranslational modification step in the P53-HIC1-SIRT1 regulatory loop. PMID: 17283066
  48. Detailed mapping of chromosome 17p deletions reveals HIC1 as a novel tumor suppressor gene candidate telomeric to TP53 in diffuse large B-cell lymphoma PMID: 17982487
  49. ARID1A/BAF250A was identified as a new HIC1 partner. PMID: 19486893
  50. Transcription factors were screened for regulation of a human HIC1 promoter reporter. Found that E2F1 strongly activates the full-length HIC1 promoter reporter. PMID: 19491197
  51. The tumor suppressor HIC1 is implicated in the transcriptional regulation of the chemokine receptor CXCR7, a key player in the promotion of tumorigenesis in a wide variety of cell types. PMID: 19525223
  52. Our data show the first valid clinical evidence of the deregulation of HIC1-SIRT1-p53 loop in lung tumorigenesis and prognosis PMID: 19649206
  53. HIC1 recruits the corepressor CtBP (C-terminal binding protein) through a phylogenetically conserved GLDLSKK motif located in a central region of the protein. This motif is a variant of the "classical" PxDLSxK found in other CtBP-interacting proteins. PMID: 12052894
  54. HIC-1 (HIC1) is a transcriptional repressor. However, in contrast to other BTB/POZ transcriptional repressors ( such as BCL6 or PLZF) it fails to recruit SMRT/NCoR-mSin3A/HDAC complexes via its BTB/POZ domain. PMID: 10611298
  55. A new major alternative promoter of HIC1 (HIC-1) is conserved in the murine locus and positively regulated by TP53. PMID: 11073960

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Subcellular Location Nucleus
Protein Families Krueppel C2H2-type zinc-finger protein family, Hic subfamily
Tissue Specificity Ubiquitously expressed with highest levels found in lung, colon, prostate, thymus, testis and ovary. Expression is absent or decreased in many tumor cells.
Database Links

HGNC: 4909

OMIM: 603825

KEGG: hsa:3090

STRING: 9606.ENSP00000314080

UniGene: Hs.695682

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