POLR3A Antibody

Datasheet

Code CSB-PA018343LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. O14802
Abbreviation RNA polymerase III subunit C1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA018343LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA018343LA01HU at dilution of 1:100

  • Chromatin Immunoprecipitation 293T(1.6*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-POLR3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers(CSB-PP018343HU) against the tRNA-Leu Anti-Codon (TAG) promoter.

Immunogen Recombinant human DNA-directed RNA polymerase III subunit RPC1 protein (392-632AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, ChIP; Recommended dilution: IHC:1:20-1:200
Relevance DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias DNA-directed RNA polymerase III subunit RPC1 (RNA polymerase III subunit C1) (EC 2.7.7.6) (DNA-directed RNA polymerase III largest subunit) (DNA-directed RNA polymerase III subunit A) (RNA polymerase III 155 kDa subunit) (RPC155) (RNA polymerase III subunit C160), POLR3A
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names POLR3A
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Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Involvement in disease Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7)
Subcellular Location Nucleus
Protein Families RNA polymerase beta' chain family
Tissue Specificity Expressed in the brain, in the cortex and the white matter (at protein level).
Database Links

HGNC: 30074

OMIM: 607694

KEGG: hsa:11128

STRING: 9606.ENSP00000361446

UniGene: Hs.436896

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