Recombinant Agrobacterium tumefaciens Bifunctional enzyme IspD/IspF (ispDF)

Code CSB-YP837653AYS
MSDS
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Source Yeast
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Code CSB-EP837653AYS
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Source E.coli
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Code CSB-EP837653AYS-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837653AYS
MSDS
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Source Baculovirus
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Code CSB-MP837653AYS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ispDF
Uniprot No.
Alternative Names
ispDF; Atu1443; AGR_C_2659; Bifunctional enzyme IspD/IspF [Includes: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; EC 2.7.7.60; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT); 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPP-synthase; MECPS; EC 4.6.1.12)]
Species
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Expression Region
1-400
Target Protein Sequence
MQESTMKFGI VIVAAGRGER AGSPEEGPKQ YRPIGGRAVI EHTLATFLRW NDACPIVVVS HADDAALLSP ILARLDAGER ITTVTGGATR QQSVLAGLEA LSSHGLTHVM IHDAVRPFVA ADMLERIAAL HRAGAGGVLP ALPVTDTLKR GLEDRVVETV SRQGLYAAQT PQSFSYSDIL DAHRAAAASG KTDFTDDASI AEWTGLTVTL TEGSVDNVKL TLKRDIAMAD EKLSHALPDV RTGNGYDVHQ LEAGDGVTLC GIFIEHDQRL KGHSDADVAL HALTDALLAT CGAGDIGDHF PPSDPQWKGA ASRIFLEHAA KVVRDNGGTI MNADVSLIAE APRIGPHRQA MREALSDMLG IALERCSVKA TTNETIGFVG RREGIAAIAT ATVVYQGRPL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF).
Gene References into Functions
  1. The time dependencies indicate that the enzyme-generated intermediates are not transferred from the IspD active site in IspDF to the active site of IspE or from the active site in IspE to the active site of the IspF module of IspDF. PMID: 16533036
Protein Families
IspD/TarI cytidylyltransferase family, IspD subfamily; IspF family
Database Links

KEGG: atu:Atu1443

STRING: 176299.Atu1443

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