Recombinant Arabidopsis thaliana Transcription factor bHLH80 (BHLH80)

Code CSB-YP863233DOA
MSDS
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Source Yeast
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Code CSB-EP863233DOA
MSDS
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Source E.coli
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Code CSB-EP863233DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863233DOA
MSDS
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Source Baculovirus
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Code CSB-MP863233DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BHLH80
Uniprot No.
Alternative Names
BHLH80; EN71; At1g35460; F12A4.2Transcription factor bHLH80; Basic helix-loop-helix protein 80; AtbHLH80; bHLH 80; Transcription factor EN 71; bHLH transcription factor bHLH080
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-259
Target Protein Sequence
MQSTHISGGS SGGGGGGGGE VSRSGLSRIR SAPATWIETL LEEDEEEGLK PNLCLTELLT GNNNSGGVIT SRDDSFEFLS SVEQGLYNHH QGGGFHRQNS SPADFLSGSG SGTDGYFSNF GIPANYDYLS TNVDISPTKR SRDMETQFSS QLKEEQMSGG ISGMMDMNMD KIFEDSVPCR VRAKRGCATH PRSIAERVRR TRISDRIRRL QELVPNMDKQ TNTADMLEEA VEYVKALQSQ IQELTEQQKR CKCKPKEEQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Results indicate a role for FLOWERING BASIC HELIX-LOOP-HELIX 1 (FBH1, AT1G35460) as a transcriptional modulator of warm temperature signals and clock responses. PMID: 25246594
  2. There are four CONSTANS transcriptional activators FBH1-4 which are related basic helix-loop-helix-type transcription factors that preferentially bind to the E-box cis-elements in the CO promoter. Overexpression of all FBH genes drastically elevated CO levels and caused early flowering regardless of photoperiod. [FBH1] PMID: 22334645
Subcellular Location
Nucleus.
Tissue Specificity
Expressed constitutively in roots, leaves, stems, and flowers.
Database Links

KEGG: ath:AT1G35460

STRING: 3702.AT1G35460.1

UniGene: At.18017

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