Recombinant Arabidopsis thaliana Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 1, chloroplastic (GGPPS1)

Code CSB-YP339503DOA
MSDS
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Source Yeast
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Code CSB-EP339503DOA
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Source E.coli
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Code CSB-EP339503DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339503DOA
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Source Baculovirus
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Code CSB-MP339503DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GGPPS1
Uniprot No.
Alternative Names
GGPPS1; GGPPS11; GGPS1; At4g36810; C7A10.550Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 1; chloroplastic; GGPP synthase 1; GGPS1; EC 2.5.1.-,; 2E,6E)-farnesyl diphosphate synthase 1; Dimethylallyltranstransferase 1; EC 2.5.1.1; Farnesyl diphosphate synthase 1; Farnesyltranstransferase 1; EC 2.5.1.29; Geranyltranstransferase 1; EC 2.5.1.10
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
57-371
Target Protein Sequence
SSVV TKEDNLRQSE PSSFDFMSYI ITKAELVNKA LDSAVPLREP LKIHEAMRYS LLAGGKRVRP VLCIAACELV GGEESTAMPA ACAVEMIHTM SLIHDDLPCM DNDDLRRGKP TNHKVFGEDV AVLAGDALLS FAFEHLASAT SSDVVSPVRV VRAVGELAKA IGTEGLVAGQ VVDISSEGLD LNDVGLEHLE FIHLHKTAAL LEASAVLGAI VGGGSDDEIE RLRKFARCIG LLFQVVDDIL DVTKSSKELG KTAGKDLIAD KLTYPKIMGL EKSREFAEKL NREARDQLLG FDSDKVAPLL ALANYIAYRQ N
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Heterodimeric geranyl(geranyl)-diphosphate (GPP) synthase large subunit. In vitro, the large subunit catalyzes mainly the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate while the small subunit alone is inactive. Upon association of the two subunits, the product profile changes and the production of gerany-diphosphate is strongly increased.
Gene References into Functions
  1. GGPPS11 is a hub isozyme required for the production of most photosynthesis-related isoprenoids. Both gene co-expression and protein-protein interaction likely contribute to the channeling of geranylgeranyl diphosphate (GGPP) by GGPPS11. PMID: 26224411
  2. GGPS1 is a key gene in the chlorophyll biosynthetic pathway. PMID: 24081146
Subcellular Location
Plastid, chloroplast.
Protein Families
FPP/GGPP synthase family
Tissue Specificity
Expressed ubiquitously.
Database Links

KEGG: ath:AT4G36810

STRING: 3702.AT4G36810.1

UniGene: At.304

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