Recombinant Arabidopsis thaliana Protein BRASSINOSTEROID INSENSITIVE 1 (BRI1), partial

Code CSB-YP517773DOA
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Source Yeast
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Code CSB-EP517773DOA
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Source E.coli
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Code CSB-EP517773DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517773DOA
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Source Baculovirus
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Code CSB-MP517773DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BRI1
Uniprot No.
Alternative Names
BRI1; At4g39400; F23K16.30Protein BRASSINOSTEROID INSENSITIVE 1; AtBRI1; EC 2.7.10.1; EC 2.7.11.1; Brassinosteroid LRR receptor kinase
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development, including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Binds brassinolide, and less effectively castasterone, but not 2,3,22,23-O-tetramethylbrassinolide or ecdysone. May be involved in a feedback regulation of brassinosteroid biosynthesis. Phosphorylates BRI1-associated receptor kinase 1 (BAK1), Transthyretin-Like protein (TTL) and SERK1 on 'Ser-299' and 'Thr-462' in vitro. May have a guanylyl cyclase activity. Phosphorylates BSK1, BSK2 and BSK3 in vitro. Phosphorylates BSK1, BSK3, BSK5, BSK6, BSK8 AND BSK11 in vitro.
Gene References into Functions
  1. identification of 83 new point mutations in BRI1, including nine mutations that exhibit an allelic series of typical bri1 phenotypes, from subtle to severe morphological alterations, is reported. PMID: 28461403
  2. These results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated plasma membrane nanodomains. PMID: 28262094
  3. PP2A regulatory B subunits mediate the binding and dephosphorylation of BRI1 and the subcellular localization of PP2A specifies its substrate selection and distinct effects on BR signaling PMID: 26517938
  4. Decreased response to exogenously applied brassinolide and altered BR marker gene expression demonstrate that zmbri1 (zea mays Bri1]-RNA interference transgenic lines have compromised Bbrassinosteroid signaling. PMID: 26162429
  5. These results suggest that brassinosteroid (BR) promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling. PMID: 25896454
  6. Internalization and vacuolar targeting of the brassinosteroid hormone receptor BRI1 are regulated by ubiquitination. PMID: 25608221
  7. These results reveal complex differential regulation of BRI1 expression, and suggest that by influencing the distribution and abundance of the receptor this regulation can enhance or attenuate brassinosteroid signalling. PMID: 24488524
  8. Endocytosis of the leucine-rich repeat receptor kinase, brassinosteroid insensitive1 (BRI1), was shown to depend on AP-2. PMID: 23975899
  9. BRI1-mediated signalling is attractive for mathematical modelling approaches to understand and interpret the spatial and temporal dynamics of signal transduction cascades in planta. PMID: 23421559
  10. multiple brassinosteroid signaling kinases (BSKs) interact with BRI1 and serve as substrates of BRI1 phosphorylation. PMID: 23496207
  11. BRI1 receptor occupancy is linked to root growth. PMID: 22802611
  12. a missense mutation in the Arabidopsis EMS-mutagenized bri1 suppressor 3 (EBS3) gene suppresses a dwarf mutant, bri1-9, the phenotypes of which are caused by ER retention and ERAD of a brassinosteroid receptor, BRASSINOSTEROID-INSENSITIVE 1 (BR1) PMID: 22733738
  13. results establish a novel assay for recombinant BRI1 transphosphorylation activity and collectively uncover a possible new link between Ca2+ and BR signalling PMID: 22309147
  14. Data demonstrate that the FLT of BRI1-GFP can be used as a non-invasive probe to analyse long-distance BL signaling in Arabidopsis seedlings. PMID: 21617383
  15. Autophosphorylation of serine-891 is one of the deactivation mechanisms that inhibit BRI1 activity and BR signaling in vivo. PMID: 22184234
  16. results demonstrate that mutation of BRI1 can enhance photosynthesis and leaf growth/vascularization and may suggest new approaches to increase whole plant carbon assimilation and growth PMID: 21795582
  17. The Brassinosteroid Insensitive1 (BRI1) receptor fused to GFP, under control of its own promoter, was quantified. PMID: 21617031
  18. structure of the Arabidopsis thaliana BRI1 ligand-binding domain, determined by X-ray diffraction at 2.5 A resolution PMID: 21666665
  19. crystal structures of BRI1(LRR) in free and brassinolide-bound forms PMID: 21666666
  20. A new bri1 allele, bri1-120, whose mutation site has not yet been found or characterized, was identified. PMID: 21219661
  21. Phosphorylation of a key tyrosine within this membrane targeting motif releases BKI1 into the cytosol following ligand perception by BRI1 PMID: 21289069
  22. Data show that bri1-R1 compensates for the protein-folding abnormalities caused by bri1-5, restoring accumulation of the receptor and its delivery to the cell surface. PMID: 20457881
  23. A bri1-9 mutant was more resistant to cold than the BRI1-overexpressing transgenic plants as well as wild-type. PMID: 20053182
  24. brassinosteroids bind directly to the 94 amino acids comprising ID-LRR22 in the extracellular domain of BRI1, and define a new binding domain for steroid hormones PMID: 15650741
  25. BRI1 is part of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE1 protein complex. PMID: 16473966
  26. a BRI1-interacting protein, BKI1,which is a negative regulator of brassinosteroid signaling is reported; BKI1 is a substrate of BRI1 kinase & limits interaction of BRI1 with its proposed coreceptor, BAK1, suggesting that BKI1 prevents activation of BRI1 PMID: 16857903
  27. Attenuation of brassinosteroid signaling enhances FLC expression and delays flowering. PMID: 17611230
  28. These results suggest that bri1-301, even with extremely impaired kinase activity, still retains partial function in regulating plant growth and development. PMID: 18332904
  29. 3 homologous brassinosteroid (BR)-signaling kinases (BSK1, BSK2 & BSK3)were identified; the BSKs are phosphorylated by BRI1; results demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction PMID: 18653891

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed ubiquitously.
Database Links

KEGG: ath:AT4G39400

STRING: 3702.AT4G39400.1

UniGene: At.27898

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