Recombinant Arthrobacter chlorophenolicus Pup--protein ligase(pafA)

Product Type Recombinant Protein
Uniprot No. B8H8L8
Species Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) (Arthrobacter chlorophenolicus)
Gene Names pafA
Protein Names Recommended name: Pup--protein ligase EC= 6.3.2.n2 Alternative name(s): Proteasome accessory factor A Pup-conjugating enzyme
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Protein Families Pup ligase/Pup deamidase family, Pup-conjugating enzyme subfamily
Database Links

KEGG: ach:Achl_1921

STRING: 452863.Achl_1921

Product Details for Different Expression Systems

Product Name Recombinant Arthrobacter chlorophenolicus Pup--protein ligase(pafA),Yeast
Code CSB-YP491348DOG
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDKRIFGIET EFGISYSSPD SRPLAPEEVA RYLFRKVVSW GRSSNVFLTN GSRLYLDVGS HPEYATAECD DLAQLIAHDR AGELILDDLV DEAQARLAAE GFNGTVYLFK NNTDSAGNSY GSHENYLIPR RGEFSRLAEI LIPFLVTRQL IAGAGKILKT PHGATYAFSQ RADHIWEGVS SATTRSRPII NTRDEPHADA EFYRRLHVIV GDSNMSETTA LMKVGTVDLV LRMIEAGVIM RDMRMENPIR SIREISHDLS GRALVRLANG RQLTALEIQQ EYLTKVTAFV RENGAHNPHV PLILDLWERT LKAIESGDTR SIDTEIDWAI KKKLMDSYRE RHGLGLDAPR IAQLDLTYHD ISRSRGLYYL LQSRGAVRRL VDDTVIKDAV DAPPQTTRAK LRGDFVRRAQ ELGRDYTVDW VHLKLNDRAH QTILCKDPFR SVDERVDALL DSMG
Expression Region 1-454
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Arthrobacter chlorophenolicus Pup--protein ligase(pafA),E.coli
Code CSB-EP491348DOG
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDKRIFGIET EFGISYSSPD SRPLAPEEVA RYLFRKVVSW GRSSNVFLTN GSRLYLDVGS HPEYATAECD DLAQLIAHDR AGELILDDLV DEAQARLAAE GFNGTVYLFK NNTDSAGNSY GSHENYLIPR RGEFSRLAEI LIPFLVTRQL IAGAGKILKT PHGATYAFSQ RADHIWEGVS SATTRSRPII NTRDEPHADA EFYRRLHVIV GDSNMSETTA LMKVGTVDLV LRMIEAGVIM RDMRMENPIR SIREISHDLS GRALVRLANG RQLTALEIQQ EYLTKVTAFV RENGAHNPHV PLILDLWERT LKAIESGDTR SIDTEIDWAI KKKLMDSYRE RHGLGLDAPR IAQLDLTYHD ISRSRGLYYL LQSRGAVRRL VDDTVIKDAV DAPPQTTRAK LRGDFVRRAQ ELGRDYTVDW VHLKLNDRAH QTILCKDPFR SVDERVDALL DSMG
Expression Region 1-454
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Arthrobacter chlorophenolicus Pup--protein ligase(pafA),Baculovirus
Code CSB-BP491348DOG
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDKRIFGIET EFGISYSSPD SRPLAPEEVA RYLFRKVVSW GRSSNVFLTN GSRLYLDVGS HPEYATAECD DLAQLIAHDR AGELILDDLV DEAQARLAAE GFNGTVYLFK NNTDSAGNSY GSHENYLIPR RGEFSRLAEI LIPFLVTRQL IAGAGKILKT PHGATYAFSQ RADHIWEGVS SATTRSRPII NTRDEPHADA EFYRRLHVIV GDSNMSETTA LMKVGTVDLV LRMIEAGVIM RDMRMENPIR SIREISHDLS GRALVRLANG RQLTALEIQQ EYLTKVTAFV RENGAHNPHV PLILDLWERT LKAIESGDTR SIDTEIDWAI KKKLMDSYRE RHGLGLDAPR IAQLDLTYHD ISRSRGLYYL LQSRGAVRRL VDDTVIKDAV DAPPQTTRAK LRGDFVRRAQ ELGRDYTVDW VHLKLNDRAH QTILCKDPFR SVDERVDALL DSMG
Expression Region 1-454
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Arthrobacter chlorophenolicus Pup--protein ligase(pafA),Mammalian cell
Code CSB-MP491348DOG
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MDKRIFGIET EFGISYSSPD SRPLAPEEVA RYLFRKVVSW GRSSNVFLTN GSRLYLDVGS HPEYATAECD DLAQLIAHDR AGELILDDLV DEAQARLAAE GFNGTVYLFK NNTDSAGNSY GSHENYLIPR RGEFSRLAEI LIPFLVTRQL IAGAGKILKT PHGATYAFSQ RADHIWEGVS SATTRSRPII NTRDEPHADA EFYRRLHVIV GDSNMSETTA LMKVGTVDLV LRMIEAGVIM RDMRMENPIR SIREISHDLS GRALVRLANG RQLTALEIQQ EYLTKVTAFV RENGAHNPHV PLILDLWERT LKAIESGDTR SIDTEIDWAI KKKLMDSYRE RHGLGLDAPR IAQLDLTYHD ISRSRGLYYL LQSRGAVRRL VDDTVIKDAV DAPPQTTRAK LRGDFVRRAQ ELGRDYTVDW VHLKLNDRAH QTILCKDPFR SVDERVDALL DSMG
Expression Region 1-454
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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