Recombinant Aspergillus terreus Polyadenylate-binding protein, cytoplasmic and nuclear(pab1) ,partial

Code CSB-YP603522APT
Size Pls inquire other sizes
Source Yeast
Code CSB-EP603522APT
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Source E.coli
Code CSB-EP603522APT-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP603522APT
Size Pls inquire other sizes
Source Baculovirus
Code CSB-MP603522APT
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names pab1
Uniprot No. Q0CR95
Species Aspergillus terreus (strain NIH 2624 / FGSC A1156)
Expression Region 1-753
Target Protein Sequence MSAEASTTPA AETPVNGTPE TSTTPAAPAA EATAAETAAP STSQPHSASL YVGELDPSVT EAMLYELFSS IGQVASIRVC RDAVTRRSLG YAYVNYNNTA DGERALEDLN YTLIKGKPCR IMWSQRDPAL RKTGQGNVFI KNLDAAIDNK ALHDTFAAFG NILSCKVAQD EFGNSKGYGF VHYETAEAAN NAIKHVNGML LNDKKVFVGH HISKKDRQSK FEEMKANFTN VYIKNLDQEI SEEEFRQMFE KFGEITSATL SRDQEGKSRG FGFVNYSTHD SAQAAVDEMN DKEVKGQKLY VGRAQKKHER EEELRKQYEA ARLEKASKYQ GVNLYVKNLT DDIDDEKLRE MFAPYGTITS AKVMRDTNIE RTQTPDSDKE KKEESKEEKP EAAEKTEEAA KESGDDQDKE NKKSDKKVLG KSKGFGFVCF SSPDEASKAV TEMNQRMING KPLYVALAQR KDVRRSQLEA SIQARNTIRQ QQAAAAAGMP QPYMQPAVFY GPGQQGFIPG GQRGGLPFAP QPGMMMGVPG GRPGQYPGPF PGQQGGRGMG PNQQIPPNFA QGIPMGAMGP GGIPNGMGYP QMGQVQFGRG AGGRGQVPGM PMGQGMRGPG YGQGRGGVPV QGQMRPGQGG RGQNAQPAAG RGEEAPAAGL TAQSLAAAPA PQQKQMLGEA LYPKIQAQQP ELAGKITGML LEMDNTELLS LLEDDEALRA KVDEALSVYD EYMKNKGTEG EAAGEAPKPK EAATEESTEE NKS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN (By similarity).
Subcellular Location Cytoplasm, Nucleus
Protein Families Polyadenylate-binding protein type-1 family
Database Links

STRING: 33178.CADATEAP00008888

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