Recombinant Bean-pod mottle virus RNA2 polyprotein

Code CSB-YP332878BFV
MSDS
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Source Yeast
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Code CSB-EP332878BFV
MSDS
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Source E.coli
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Code CSB-EP332878BFV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP332878BFV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP332878BFV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
RNA2 polyprotein; Genome polyprotein M; M RNA polyprotein; Middle component RNA polyprotein; P2) [Cleaved into: VP58; P58); Movement protein; MP); Large capsid protein; LCP; Coat protein VP42; L subunit; Large coat protein); Small capsid protein precursor; S subunit); Mature small capsid protein; SCP; Coat protein VP22; Small capsid protein; N-terminus part; Small coat protein; N-terminus part); Small capsid protein C-terminus part; Small coat protein C-terminus part)]
Species
Bean-pod mottle virus (strain Kentucky G7) (BPMV)
Expression Region
1-446
Target Protein Sequence
MFASFIFSGD NKLTEKTIFN CGDLDILVVY YTIATQFRKF LPHYIRWHLY TLLIYILPSF LTTEIKYKRN LSNIHISGLF YDNRFKFWTK HDKNLALTEE EKMEVIRNRG IPADVLAKRA HEFEKHVAHE SLKDQIPAVD KLYSTKVNKF AKIMNLRQSV VGDLKLLTDG KLYEGKHIPV SNISAGENHV VQIPLMAQEE ILSSSASDFK TAMVSKSSKP QATAMHVGAI EIIIDSFASP DCNIVGAMLL VDTYHTNPEN AVRSIFVAPF RGGRPIRVVT FPNTIVQIEP DMNSRFQLLS TTTNGDFVQG KDLAMVKVNV ACAAVGLTSS YTPTPLLESG LQKDRGLIVE YFGRMSYVAH NINQPQEKDL LEGNFSFDIK SRSRLEKVSS TKAQFVSGRT FKYDIIGAGS QSSEELSEEK IQGKAKQVDA RLRQRIDPQY NEVQAQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication.; Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner.; The cleavable C-terminus of small capsid protein seems to be involved in viral assembly and RNA packaging. After virus assembly, these amino acids are cleaved off during the normal maturation of the virus. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.; Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.; Together with the mature small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the mature small capsid protein. The large capsid protein interacts with the viral RNA.; Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the mature small capsid protein. The mature small capsid protein forms the turrets at the fivefold axes of the viral particle.
Subcellular Location
[Movement protein]: Host cell junction, host plasmodesma.; [Large capsid protein]: Virion.; [Mature small capsid protein]: Virion.
Protein Families
Comoviridae genome polyprotein M family
Database Links

KEGG: vg:956616

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