Recombinant Bradyrhizobium japonicum Amino acid--[acyl-carrier-protein] ligase 1 (bll0957)

Code CSB-YP804691BVW
MSDS
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Source Yeast
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Code CSB-EP804691BVW
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Source E.coli
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Code CSB-EP804691BVW-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804691BVW
MSDS
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Source Baculovirus
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Code CSB-MP804691BVW
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bll0957
Uniprot No.
Alternative Names
bll0957Amino acid--[acyl-carrier-protein] ligase 1; EC 6.2.1.n2; Amino acid:[carrier-protein] ligase [AMP forming] 1; aa:CP ligase 1; Aminoacyl-[acyl-carrier-protein] synthetase 1; L-glycine:[acyl-carrier-protein] ligase 1
Species
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Expression Region
1-326
Target Protein Sequence
MNIAVLPNSP DTAPQIADPL DHLADKLFHS MGSDGVYART ALYESIVERL AALITSHREA GTEALRFPPV MSRAQLEKSG YLKSFPNLLG CVCGLHGTER EINAAVSRFD AGGDWTTSLS PADLVLSPAA CYPVYPIAAS RGPLPKGGLR FDVAADCFRR EPSKHLDRLQ SFRMREYVCI GTPDDVSDFR ERWMVRAQAI ARDLGLTFRV DYASDPFFGR VGQMKAVSQK QQQLKFELLI PLRSEEQPTA CMSFNYHREH FGTTWGIQDA NGEPAHTGCV AFGMDRLAVA MFHTHGTDLS AWPAKVRDIL GLQPHVAAGA HGEGWR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the ATP-dependent activation of L-glycine and its transfer to the phosphopantetheine prosthetic group covalently attached to the vicinal carrier protein bsr0959 of yet unknown function. May participate in nonribosomal peptide synthesis or related processes. L-alanine is a poor substrate whereas L-serine or D-amino acids are not substrates for ATP-dependent activation. Does not display tRNA aminoacylation activity.
Protein Families
Class-II aminoacyl-tRNA synthetase family, Amino acid--[acyl-carrier-protein] ligase subfamily
Database Links

KEGG: bja:bll0957

STRING: 224911.bll0957

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