Name: Recombinant Calloselasma rhodostoma Zinc metalloproteinase/disintegrin, partial
Brand: CUSABIO
SKU: 341149CBG

Product Details

Purity

>85% (SDS-PAGE)

Uniprot No.

P30403

Alternative Names

; Zinc metalloproteinase/disintegrin [Cleaved into: Snake venom metalloproteinase rhodostoxin; SVMP; EC 3.4.24.-; Hemorrhagic protein); Disintegrin rhodostomin; RHO; RHOD; Disintegrin kistrin; Platelet aggregation activation inhibitor)]

Species

Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)

Expression Region

189-391

Target Protein Sequence

NH EIKRHVDIVV VVDSRFCTKH SNDLEVIRKF VHEVVNAIIE SYKYMHFGIS LVNLETWCNG DLINVQEDSY ETLKAFGKWR ESDLIKHVNH SNAQFLMDMK FIKNIIGKAY LDSICDPERS VGIVQNYHGI TLNVAAIMAH EMGHNLGVRH DGEYCTCYGS SECIMSSHIS DPPSKYFSNC SYYQFWKYIE NQNPQCILNK P

Protein Length

partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

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■ Q&A

We are looking for a source of Disintegrin rhodostomin which appears to be one chain (aa 408–475) of Zinc metalloproteinase/disintegrin.
https://www.uniprot.org/uniprot/P30403#PRO_0000028959
I can only find a recombinant corresponding to the other chain Snake venom metalloproteinase rhodostoxin (aa 189–391)
CSB-EP341149CBG; CSB-YP341149CBG; CSB-BP341149CBG; CSB-MP341149CBG Recombinant Calloselasma rhodostoma Zinc metalloproteinase/disintegrin (RHOD)

Do you have anything corresponding to the Disintegrin rhodostomin chain?

Thanks for your inquiry.
Although we only list the protein corresponding to the other chain Snake venom metalloproteinase rhodostoxin (aa 189-391), please feel free to check with us if you can't find the desired product on our website.
We can provide the protein corresponding to the chain (aa 408-475) of Zinc metalloproteinase/disintegrin.
https://www.uniprot.org/uniprot/P30403
Recombinant Calloselasma rhodostoma Zinc metalloproteinase/disintegrin,partial
CSB-YP341149CBG >> Yeast
CSB-EP341149CBG >> E.coli
CSB-BP341149CBG >> Baculovirus
CSB-MP341149CBG >> Mammalian cell
Expression Region: 189-391aa; Partial, provide the Snake venom metalloproteinase rhodostoxin chain
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

NHEIKRHVDIVVVVDSRFCTKHSNDLEVIRKFVHEVVNAIIESYKYMHFGISLVNLETWCNGDLINVQEDSYETLKAFGKWRESDLIKHVNHSNAQFLMDMKFIKNIIGKAYLDSICDPERSVGIVQNYHGITLNVAAIMAHEMGHNLGVRHDGEYCTCYGSSECIMSSHISDPPSKYFSNCSYYQFWKYIENQNPQCILNKP

Recombinant Calloselasma rhodostoma Zinc metalloproteinase/disintegrin,partial
CSB-YP341149CBG1 >> Yeast
CSB-EP341149CBG1 >> E.coli
CSB-BP341149CBG1 >> Baculovirus
CSB-MP341149CBG1 >> Mammalian cell
Expression Region: 408-475aa; Partial, provide the Disintegrin rhodostomin chain
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

GKECDCSSPENPCCDAATCKLRPGAQCGEGLCCEQCKFSRAGKICRIPRGDMPDDRCTGQSADCPRYH

Target Background

Function

impairs hemostasis in the envenomed animal.; inhibit platelet aggregation induced by ADP, thrombin, platelet-activating factor and collagen. Acts by inhibiting fibrinogen interaction with platelet receptors alpha-IIb/beta-3 (ITGA2B/ITGB3).

Subcellular Location

Secreted.

Protein Families

Venom metalloproteinase (M12B) family, P-II subfamily, P-IIa sub-subfamily

Tissue Specificity

Expressed by the venom gland.

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Code	CSB-YP341149CBG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP341149CBG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP341149CBG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP341149CBG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP341149CBG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Calloselasma rhodostoma Zinc metalloproteinase/disintegrin, partial

Product Details

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