Recombinant Dictyostelium discoideum Probable methyltransferase DDB_G0287769, mitochondrial (DDB_G0287769)

Code CSB-YP681627DKK
MSDS
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Source Yeast
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Code CSB-EP681627DKK
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Source E.coli
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Code CSB-EP681627DKK-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP681627DKK
MSDS
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Source Baculovirus
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Code CSB-MP681627DKK
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ndufaf5
Uniprot No.
Alternative Names
ndufaf5; DDB_G0287769; Arginine-hydroxylase NDUFAF5; mitochondrial; EC 1.-.-.-; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; Putative methyltransferase NDUFAF5; EC 2.1.1.-
Species
Dictyostelium discoideum (Slime mold)
Expression Region
26-436
Target Protein Sequence
SNYTK MTIFDTNVKT IQKNNTVTNV DDPKHYDYLM NEVADRLADR ILDIKDIKCG NVLDFGSRNG ALFKYIQEKG AKIDKYYMVE SSKELLYRDD NNVSQENEDD NNNNKVKPTK ILVNSLEDKI EGIEDQSLDL IISNLSLHWV NDLPGVFGGL KRLLKPNGVF LASLFGEDTL MELKDSLYLA EIEREGGFSP HVSPFTKISD IGNILSKNRY TLPTVDTEKI TINYDNMFVL MRDLQNMGEN NAILKRRNYT SKDTFLAASA IYKHLYGNED NNSIPATFQI IYLIGWAPHE SQQKPLQRGS AKKHFSEISG TSSFGYKFDN DSSIPSILTN ENNSVTLSQQ QQQQGIEPQQ SNDDSINEPF PKTDDFVIKR LDYHGNFHFE KQQQQQQQQD QNKESSDEIN KNKDDK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. Probably acts as an arginine hydroxylase. May also have methyltransferase activity.
Gene References into Functions
  1. Ndufaf5-deficient Dictyostelium shows an increase in autophagy. PMID: 23536703
Subcellular Location
Mitochondrion.
Protein Families
Methyltransferase superfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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