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Recombinant Dictyostelium discoideum Sphingosine-1-phosphate lyase (sglA), partial

Recombinant Dictyostelium discoideum Sphingosine-1-phosphate lyase (sglA), partial

Code	CSB-YP703834DKK1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP703834DKK1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP703834DKK1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP703834DKK1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP703834DKK1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

sglA

Uniprot No.

Q54RV9

Alternative Names

sglA; DDB_G0282819; Sphingosine-1-phosphate lyase; S1P lyase; S1PL; SP-lyase; SPL; Sphingosine-1-phosphate aldolase

Species

Dictyostelium discoideum (Slime mold)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Sphingosine-1-phosphate (S1P) probably acts intracellularly as a second messenger perhaps by promoting cell proliferation; the absence of S1P lyase increases its concentration. This leads to increased lateral pseudopod formation as well as defects in the efficiency of chemotaxis. Overexpression of S1P lyase causes decreased growth rates, entry into stationary phase at lower cell density and increased sensitivity to the antitumor agents cisplatin and carboplatin; these effects are more pronounced in cells that express more enzyme.

Subcellular Location

Endoplasmic reticulum membrane; Single-pass membrane protein.

Protein Families

Group II decarboxylase family, Sphingosine-1-phosphate lyase subfamily

Database Links

KEGG: ddi:DDB_G0282819

STRING: 44689.DDB0214888

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