Recombinant Enterobacter sp. Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp(gcp)

Code CSB-YP395368EIZ
Size Pls inquire other sizes
Source Yeast
Code CSB-EP395368EIZ
Size Pls inquire other sizes
Source E.coli
Code CSB-EP395368EIZ-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP395368EIZ
Size Pls inquire other sizes
Source Baculovirus
Code CSB-MP395368EIZ
Size Pls inquire other sizes
Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names tsaD
Uniprot No. A4WEJ9
Species Enterobacter sp. (strain 638)
Expression Region 1-337
Target Protein Sequence MRVLGIETSC DETGIAIYDD EKGLLANQLY SQVKLHADYG GVVPELASRD HVRKTVPLIQ AALKESGLTA KDIDAVAYTA GPGLVGALLV GATIGRSLAF AWDVPAIPVH HMEGHLLAPM LEENPPEFPF VALLVSGGHT QLISVTGIGE YELLGESIDD AAGEAFDKTA KLLGLDYPGG PMLSKLASQG VEKRFVFPRP MTDRPGLDFS FSGLKTFAAN TIRNNENDDQ TRADIARAFE DAVVDTLMIK CKRALDQTGF TRLVMAGGVS ANRTLRTRLE EMMQKRRGEV FYARPEFCTD NGAMIAYAGM VRVKGGATAD LSVSVRPRWP LAELPAA
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
Still Have Questions? Start a Chat

Target Data

Function Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.
Subcellular Location Cytoplasm
Protein Families KAE1 / TsaD family
Database Links

KEGG: ent:Ent638_3470

STRING: 399742.Ent638_3470

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.