Recombinant Escherichia coli Uncharacterized protein ygjK (ygjK)

Code CSB-YP337144ENV
MSDS
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Source Yeast
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Code CSB-EP337144ENV
MSDS
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Source E.coli
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Code CSB-EP337144ENV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP337144ENV
MSDS
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Source Baculovirus
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Code CSB-MP337144ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ygjK
Uniprot No.
Alternative Names
ygjK; b3080; JW3051; Glucosidase YgjK; EC 3.2.1.-
Species
Escherichia coli (strain K12)
Expression Region
23-783
Target Protein Sequence
ANADNYKN VINRTGAPQY MKDYDYDDHQ RFNPFFDLGA WHGHLLPDGP NTMGGFPGVA LLTEEYINFM ASNFDRLTVW QDGKKVDFTL EAYSIPGALV QKLTAKDVQV EMTLRFATPR TSLLETKITS NKPLDLVWDG ELLEKLEAKE GKPLSDKTIA GEYPDYQRKI SATRDGLKVT FGKVRATWDL LTSGESEYQV HKSLPVQTEI NGNRFTSKAH INGSTTLYTT YSHLLTAQEV SKEQMQIRDI LARPAFYLTA SQQRWEEYLK KGLTNPDATP EQTRVAVKAI ETLNGNWRSP GGAVKFNTVT PSVTGRWFSG NQTWPWDTWK QAFAMAHFNP DIAKENIRAV FSWQIQPGDS VRPQDVGFVP DLIAWNLSPE RGGDGGNWNE RNTKPSLAAW SVMEVYNVTQ DKTWVAEMYP KLVAYHDWWL RNRDHNGNGV PEYGATRDKA HNTESGEMLF TVKKGDKEET QSGLNNYARV VEKGQYDSLE IPAQVAASWE SGRDDAAVFG FIDKEQLDKY VANGGKRSDW TVKFAENRSQ DGTLLGYSLL QESVDQASYM YSDNHYLAEM ATILGKPEEA KRYRQLAQQL ADYINTCMFD PTTQFYYDVR IEDKPLANGC AGKPIVERGK GPEGWSPLFN GAATQANADA VVKVMLDPKE FNTFVPLGTA ALTNPAFGAD IYWRGRVWVD QFWFGLKGME RYGYRDDALK LADTFFRHAK GLTADGPIQE NYNPLTGAQQ GAPNFSWSAA HLYMLYNDFF RKQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Glucoside hydrolase that cleaves the alpha-1,3-glucosidic linkage in nigerose. Has very low activity towards maltooligosaccharides, soluble starch, nigerotriose, kojibiose and trehalose.
Gene References into Functions
  1. Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule. These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes PMID: 27688023
  2. Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method and diffraction data were collected to 1.8 A resolution PMID: 15213393
Protein Families
Glycosyl hydrolase 63 family
Database Links
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