Recombinant F420-0:gamma-glutamyl ligase (cofE)

Code CSB-YP887277MVN
MSDS
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Source Yeast
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Code CSB-EP887277MVN
MSDS
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Source E.coli
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Code CSB-EP887277MVN-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887277MVN
MSDS
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Source Baculovirus
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Code CSB-MP887277MVN
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
fbiB
Uniprot No.
Alternative Names
fbiB; ML0758Bifunctional F420 biosynthesis protein FbiB [Includes: Coenzyme F420:L-glutamate ligase; EC 6.3.2.31; EC 6.3.2.34; Coenzyme F420-0:L-glutamate ligase; Coenzyme F420-1:gamma-L-glutamate ligase); Dehydro-coenzyme F420-0 reductase; EC 1.3.8.-)]
Species
Mycobacterium leprae (strain TN)
Expression Region
1-457
Target Protein Sequence
MTSSDSHRSA PSPEHGTAST IEILPVAGLP EFRPGDDLSA ALAAAAPWLR DGDVVVVTSK VVSKCEGRLV PAPEDTRGRN ELRRKLINDE TIRVLARKGR TLIIENGLGL VQAAAGVDGS NVGRGELALL PVNPDASAAV LRIGLRAMLG VNVAVVITDT MGRAWRNGQT DVAIGAAGLA VLHNYSGAVD RYGNELVVTE IAVADEVAAA TDLVKGKLTA MPVAVVRGLS PTDDGSTAQH LLRNGPDDLF WLGTTEALEL GRQQAQLLRR SVRQFSDEPI AAELIETAVA EALTAPAPHH TRPVRFVWLQ TPAVRTRLLD RMADKWRLDL ASDALPADAI AQRVARGQIL YDAPEVIIPF MVPDGAHAYP DAARASAEHT MFIVAVGAAV QALLVALAVR GLGSCWIGST IFADDLVRAE LELPADWEPL GAIAIGYAHE PTDLREPVRV ADLLLRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional enzyme that catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2-dependent reduction of dehydro-F420-0 to form F420-0.
Protein Families
CofE family
Database Links

KEGG: mle:ML0758

STRING: 272631.ML0758

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