Recombinant Guinea pig Platelet-activating factor acetylhydrolase (PLA2G7)

Code CSB-YP018105GU
MSDS
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Source Yeast
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Code CSB-EP018105GU
MSDS
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Source E.coli
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Code CSB-EP018105GU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018105GU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP018105GU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PLA2G7
Uniprot No.
Alternative Names
PLA2G7; PAFAHPlatelet-activating factor acetylhydrolase; PAF acetylhydrolase; EC 3.1.1.47; 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycerophosphocholine esterase; LDL-associated phospholipase A2; LDL-PLA(2); PAF 2-acylhydrolase
Species
Cavia porcellus (Guinea pig)
Expression Region
22-436
Target Protein Sequence
FDWRDLDPV TYIQSSVWIQ RIQSELLITS FGHTTIPKGN GPYSVGCTDL MSGYTNQSSF LRLYYPSQDN DFPDALWIPN EEYFQGLTET LGASSFLGKL LKLLYGSVKV PAKWNSPLKT GEKYPLIIFS HGLGAFRSIY SAIGIELASH GFIVAAVEHR DESAAATYYF QDAPAAESGN RSWIYYKVGN LETEERKRQL RQRGEECSQA LSWLLSIDEG EPVKNVLDLN FDIQQLKGSL DRSKVAIIGH SFGGATVIQT LSEDQRFRCG IALDPWMFPV GEDVHSKIPQ PLFFINSEYF QSANDTKKIE KFYQPQKERK MIAVKGSVHH NFVDFTFATG KIIGQMLSLK GKIDSEVAMD LINKASLAFL QKYLGLDKNF DQWNSLMEGD DENLIPEFTI PTTMQSSTGT EQRNPD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in lipoprotein particles and uncontrolled proinflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
Subcellular Location
Secreted, extracellular space.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Plasma.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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