Recombinant Halomonas elongata Diaminobutyrate--2-oxoglutarate transaminase (ectB)

Code CSB-YP524424HTX
MSDS
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Source Yeast
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Code CSB-EP524424HTX
MSDS
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Source E.coli
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Code CSB-EP524424HTX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP524424HTX
MSDS
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Source Baculovirus
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Code CSB-MP524424HTX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ectB
Uniprot No.
Alternative Names
ectB; HELO_2589Diaminobutyrate--2-oxoglutarate transaminase; EC 2.6.1.76; DABA aminotransferase; Diaminobutyrate--2-oxoglutarate aminotransferase; L-2,4-diaminobutyric acid transaminase
Species
Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)
Expression Region
1-421
Target Protein Sequence
MQTQILERME SDVRTYSRSF PVVFTKARNA RLTDEEGREY IDFLAGAGTL NYGHNNPHLK QALLDYIDSD GIVHGLDFWT AAKRDYLETL EEVILKPRGL DYKVHLPGPT GTNAVEAAIR LARVAKGRHN IVSFTNGFHG VTMGALATTG NRKFREATGG VPTQAASFMP FDGYLGSSTD TLDYFEKLLG DKSGGLDVPA AVIVETVQGE GGINVAGLEW LKRLESICRA NDILLIIDDI QAGCGRTGKF FSFEHAGITP DIVTNSKSLS GYGLPFAHVL MRPELDKWKP GQYNGTFRGF NLAFATAAAA MRKYWSDDTF ERDVQRKARI VEERFGKIAA WLSENGIEAS ERGRGLMRGI DVGSGDIADK ITHQAFENGL IIETSGQDGE VVKCLCPLTI PDEDLVEGLD ILETSTKQAF S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes reversively the conversion of L-aspartate beta-semialdehyde (ASA) to L-2,4-diaminobutyrate (DABA) by transamination with L-glutamate. Seems to use L-glutamate specifically as the amino group donor to ASA, as it is not active with L-alanine, L-glutamine, L-aspartate and L-lysine, and is only poorly active with L-homoserine. In the reverse reaction, gamma-aminobutyric acid (GABA) and L-ornithine can also be used as amino group donors to 2-oxoglutarate, but with a reduced activity compared to that with DABA.
Protein Families
Class-III pyridoxal-phosphate-dependent aminotransferase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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