Code | CSB-EP001543HU |
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Size | $224 |
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Insertion of the gene encoding the Human AKR1C2 protein (1-323aa) into a plasmid vector results in the formation of recombinant plasmid, which is then introduced into e.coli cells. Positive e.coli cells are selected relying on their ability to survive in the presence of a specific antibiotic. The e.coli cells containing the recombinant plasmid are cultured under conditions conducive to the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is attached to the protein. Following expression, the recombinant Human AKR1C2 protein is isolated and purified from the cell lysate through affinity purification. The resultant recombinant Human AKR1C2 protein is analyzed using denaturing SDS-PAGE, revealing a purity greater than 90%.
Applications : Drug treatment
Review: For the experiment with addition of constant amounts of ibuprofen (30 µM) as inhibitor of AKR1C2 no significant changes in the Km-values were observed, while the maximum velocity decreased when ibuprofen was added.
By Anonymous
According to the data sheet, concentration of this protein is 0.2 mg/ml. Does the indicated concentration refer to the pure protein without taq or for the whole protein (protein + tag).
I am interested in the product CSB-EP001543HU, and have the following questions:
Was the enzyme tested for functional experiments and if yes how is its activity and against which substance tested? As it is shipped in liquid condition I was wondering in which concentration the enzyme is in solution?