Recombinant Human Alpha N-terminal protein methyltransferase 1B (METTL11B)

Code CSB-YP722791HU
MSDS
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Source Yeast
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Code CSB-EP722791HU
MSDS
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Source E.coli
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Code CSB-EP722791HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP722791HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP722791HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
METTL11B
Uniprot No.
Alternative Names
METTL11B; C1orf184; NRMT2; Alpha N-terminal protein methyltransferase 1B; EC 2.1.1.299; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B
Species
Homo sapiens (Human)
Expression Region
1-283
Target Protein Sequence
MAHRGAHFAF RSRWQKTDDE LCRHSMSFIL HKAIRNDFFQ SYLYLLEKIP LVKLYALTSQ VINGEMQFYA RAKLFYQEVP ATEEGMMGNF IELSSPDIQA SQKFLRKFVG GPGRAGTDCA LDCGSGIGRV SKHVLLPVFN SVELVDMMES FLLEAQNYLQ VKGDKVESYH CYSLQEFTPP FRRYDVIWIQ WVSGHLTDKD LLAFLSRCRD GLKENGIIIL KDNVAREGCI LDLSDSSVTR DMDILRSLIR KSGLVVLGQE KQDGFPEQCI PVWMFALHSD RHS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Alpha N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. Predominantly functions as a mono-methyltransferase but is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide (in vitro). May activate NTMT1 by priming its substrates for trimethylation.
Gene References into Functions
  1. Concurrent expression of NRMT1 and NRMT2 accelerates the production of trimethylation, and we propose that NRMT2 activates NRMT1 by priming its substrates for trimethylation. PMID: 24090352
Subcellular Location
Nucleus.
Protein Families
Methyltransferase superfamily, NTM1 family
Database Links

HGNC: 31932

KEGG: hsa:149281

STRING: 9606.ENSP00000408058

UniGene: Hs.553612

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