Recombinant Human Arrestin domain-containing protein 3 (ARRDC3)

Code CSB-YP839293HU
MSDS
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Source Yeast
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Code CSB-EP839293HU
MSDS
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Source E.coli
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Code CSB-EP839293HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839293HU
MSDS
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Source Baculovirus
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Code CSB-MP839293HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARRDC3
Uniprot No.
Alternative Names
ARRDC3; KIAA1376; Arrestin domain-containing protein 3; TBP-2-like inducible membrane protein; TLIMP
Species
Homo sapiens (Human)
Expression Region
1-414
Target Protein Sequence
MVLGKVKSLT ISFDCLNDSN VPVYSSGDTV SGRVNLEVTG EIRVKSLKIH ARGHAKVRWT ESRNAGSNTA YTQNYTEEVE YFNHKDILIG HERDDDNSEE GFHTIHSGRH EYAFSFELPQ TPLATSFEGR HGSVRYWVKA ELHRPWLLPV KLKKEFTVFE HIDINTPSLL SPQAGTKEKT LCCWFCTSGP ISLSAKIERK GYTPGESIQI FAEIENCSSR MVVPKAAIYQ TQAFYAKGKM KEVKQLVANL RGESLSSGKT ETWNGKLLKI PPVSPSILDC SIIRVEYSLM VYVDIPGAMD LFLNLPLVIG TIPLHPFGSR TSSVSSQCSM NMNWLSLSLP ERPEAPPSYA EVVTEEQRRN NLAPVSACDD FERALQGPLF AYIQEFRFLP PPLYSEIDPN PDQSADDRPS CPSR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.
Gene References into Functions
  1. correlation analysis indicated that the expression of ARRDC3 was negatively correlated with ITGbeta4 in clinical prostate cancer (PCa) tissues and cell lines. Our data revealed that ARRDC3 can serve as a tumor suppressor to inhibit PCa progression and an independent marker to predict the risk of biochemical recurrence and metastasis after radical resection of PCa. PMID: 28782483
  2. For the in vivo experiment, miR-182-5p overexpression also promoted the growth and progression of prostate cancer tumors. In this regard, we suggest that miR-182-5p may be a key androgen receptor-regulated factor that contributes to the development and metastasis of Chinese prostate cancers and may be a potential target for the early diagnosis and therapeutic studies of prostate cancer. PMID: 27109471
  3. ARRDC3 functions as a switch to modulate the endosomal residence time and subsequent intracellular signaling of the beta2AR PMID: 27226565
  4. We found that ARRDC3 is required for ALIX ubiquitination induced by activation of PAR1 PMID: 26490116
  5. Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ARRDC3 in invasive ductal breast carcinoma. PMID: 25148870
  6. The ARRDC3 is one of six known human alpha-arrestins, and has been implicated in the downregulation of the beta2-adrenergic receptor (beta2AR). PMID: 25220262
  7. High affinity binding of full-length ARRDC3 and Nedd4 is driven by the avid interaction of both PPXY motifs with either the WW2-WW3 or WW3-WW4 combinations. PMID: 24379409
  8. Beta 2 adrenergic receptor endocytosis requires beta-arrestin2 but not ARRDC3. PMID: 23208550
  9. single-nucleotide polymorphisms in ARRDC3, FLT1, and SKAP1 were significant predictors for survival androgen-deprivation therapy in prostate cancer patients. PMID: 21652578
  10. The obesity risk locus contains a single gene, arrestin domain-containing 3 (ARRDC3), which interacts directly with beta-adrenergic receptors and is gender-specific. PMID: 21982743
  11. Data show that ARRDC3 interacts with NEDD4 through two conserved PPXY motifs and recruits NEDD4 to the activated beta2-adrenergic receptor. PMID: 20559325
  12. ARRDC3 directly binds to a phosphorylated form of ITGbeta4 leading to its internalization, ubiquitination and ultimate degradation, and suppress breast cancer progression. PMID: 20603614
  13. [Thioredoxin-binding protein-2-like inducible membrane protein, TLIMP] TLIMP, a novel VD3- or PPARgamma ligand-inducible membrane-associated protein, plays a regulatory role in cell proliferation and PPARgamma activation. PMID: 16269462
  14. Txnip regulates cellular metabolism independent of its binding to thioredoxin and the arrestin domains are crucial structural elements in metabolic functions of alpha-arrestin proteins PMID: 19605364

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Endosome. Early endosome.
Protein Families
Arrestin family
Tissue Specificity
Highly expressed in skeletal muscle, placenta, kidney, lung, liver, blood, adrenal gland, lymph node, mammary gland, thyroid, and trachea. Very low levels in colon, thymus, spleen, small intestine, bladder and bone marrow. Strong expression in differentia
Database Links

HGNC: 29263

OMIM: 612464

KEGG: hsa:57561

STRING: 9606.ENSP00000265138

UniGene: Hs.24684

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