Recombinant Human Atherin (SAMD1)

Code CSB-YP761438HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP761438HU
MSDS
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Source E.coli
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Code CSB-EP761438HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP761438HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP761438HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SAMD1
Uniprot No.
Alternative Names
Atherin; SAM domain containing 1; SAM domain-containing protein 1; SAMD1; SAMD1_HUMAN; Sterile alpha motif domain containing 1; Sterile alpha motif domain-containing protein 1
Species
Homo sapiens (Human)
Expression Region
1-538
Target Protein Sequence
MAGPPALPPP ETAAAATTAA AASSSAASPH YQEWILDTID SLRSRKARPD LERICRMVRR RHGPEPERTR AELEKLIQQR AVLRVSYKGS ISYRNAARVQ PPRRGATPPA PPRAPRGAPA AAAAAAPPPT PAPPPPPAPV AAAAPARAPR AAAAAATAPP SPGPAQPGPR AQRAAPLAAP PPAPAAPPAV APPAGPRRAP PPAVAAREPP LPPPPQPPAP PQQQQPPPPQ PQPPPEGGAV RAGGAARPVS LREVVRYLGG SGGAGGRLTR GRVQGLLEEE AAARGRLERT RLGALALPRG DRPGRAPPAA SARPSRSKRG GEERVLEKEE EEDDDEDEDE EDDVSEGSEV PESDRPAGAQ HHQLNGERGP QSAKERVKEW TPCGPHQGQD EGRGPAPGSG TRQVFSMAAM NKEGGTASVA TGPDSPSPVP LPPGKPALPG ADGTPFGCPP GRKEKPSDPV EWTVMDVVEY FTEAGFPEQA TAFQEQEIDG KSLLLMQRTD VLTGLSIRLG PALKIYEHHI KVLQQGHFED DDPDGFLG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Unmethylated CpG islands (CGIs)-binding protein which localizes to H3K4me3-decorated CGIs, where it acts as a transcriptional repressor. Tethers L3MBTL3 to chromatin and interacts with the KDM1A histone demethylase complex to modulate H3K4me2 and H3K4me3 levels at CGIs. Plays a role in atherogenesis by binding with LDL on cell surface and promoting LDL oxidation which leads to the formation of foam cell.
Gene References into Functions
  1. The nuclear importin IPO5 was identified as a novel interacting protein of SMAD1. Overexpression of IPO5 in various cell lines specifically increases nuclear localization of BMP receptor-activated SMADs (R-SMADs) confirming a functional relationship between IPO5 and BMP but not TGF-beta R-SMADs. PMID: 27703004
  2. atherin is present only in atherosclerotic lesions, not in normal intima PMID: 16159594
Subcellular Location
Nucleus. Chromosome. Secreted.
Tissue Specificity
Expressed in atherosclerotic lesions, not in normal intima. Expressed in foam cells.
Database Links

HGNC: 17958

KEGG: hsa:90378

STRING: 9606.ENSP00000431971

UniGene: Hs.140309

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