Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

NDST1

Uniprot No.

P52848

Research Area

Immunology

Alternative Names

NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1) [Includes: Heparan sulfate N-deacetylase 1; EC 3.-.-.-); Heparan sulfate N-sulfotransferase 1; EC 2.8.2.-)]

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

40-556aa

Target Protein Sequence

GWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPVSAPQPMAAGEKGLLHSLSAADTGFLEPGKGGEA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

73.5kDa

Protein Length

Partial of Isoform 2

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human NDST1 was expressed with the amino acid range of 40-556. The theoretical molecular weight of the NDST1 protein is 73.5 kDa. Expression of this NDST1 protein is conducted in e.coli. The NDST1 coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant NDST1 protein in following stages of expression and purification.

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1) is a crucial enzyme involved in the biosynthesis of heparan sulfate, a complex polysaccharide that plays essential roles in various biological processes. NDST1 possesses two distinct enzymatic activities: N-deacetylase and N-sulfotransferase. In the N-deacetylase activity, it catalyzes the removal of acetyl groups from N-acetylglucosamine residues, while in the N-sulfotransferase activity, it transfers sulfate groups to the resulting amino groups. These modifications contribute to the structural diversity of heparan sulfate, influencing its interactions with various proteins. NDST1 is essential for embryonic development, cell signaling, and extracellular matrix organization. Research on NDST1 spans areas such as developmental biology, cancer biology, and therapeutic interventions targeting heparan sulfate-related processes.

Target Background

Function

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan.

Gene References into Functions

Compound heterozygous mutations in NDST1 were identified, in the heparan sulfate N deacetylatase domain of one allele and the sulfotransferase domain of the other allele. This report expands the phenotypic spectrum of Ndst1 deficiency in humans. PMID: 28211985
Among the genes enriched in this screening, the authors found that TM9SF2 is critical for N-sulfation of heparan sulfate and therefore for chikungunya virus infection because it is involved in the proper localization and stability of NDST1. PMID: 28404855
Our data confirm NDST1 mutations as a cause of autosomal recessive intellectual disability with a distinctive phenotype, and support an important function of NDST1 in human development. PMID: 25125150
demonstrate the essential role of domain cooperation within NDST-1 in producing HS with specific domain structures PMID: 26109066
Upregulation of NDST1 is associated with chemoresistance in breast cancer. PMID: 25156775
These findings establish NDST1 as a target of miR-24 and demonstrate how such NDST1 suppression in endothelial cells results in reduced responsiveness to VEGFA PMID: 23884416
MicroRNA-191 targets N-deacetylase/N-sulfotransferase 1 and promotes cell growth in human gastric carcinoma cell line MGC803 PMID: 21947487
Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1. PMID: 20129923
Altered expression of NDST-1 mRNA in glomerular basement membrane in puromycin aminonucleoside nephrosis. PMID: 14966466
effect of targeted inactivation of the Ndst1 gene on the inflammatory response associated with allergic inflammation PMID: 19710461
the expression of the NDST-1 isoform was approximately equal at all stages of mast cell maturation PMID: 19915053

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Involvement in disease

Mental retardation, autosomal recessive 46 (MRT46)

Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Sulfotransferase 1 family, NDST subfamily

Tissue Specificity

Widely expressed. Expression is most abundant in heart, liver and pancreas.

Database Links

HGNC: 7680

OMIM: 600853

KEGG: hsa:3340

STRING: 9606.ENSP00000261797

UniGene: Hs.222055

Code	CSB-EP015614HU
Abbreviation	Recombinant Human NDST1 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1), partial

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