Recombinant Human Centromere protein W (CENPW)

Code CSB-YP701863HU
MSDS
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Source Yeast
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Code CSB-EP701863HU
MSDS
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Source E.coli
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Code CSB-EP701863HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP701863HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP701863HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CENPW
Uniprot No.
Alternative Names
C6ORF173; cancer upregulated gene 2; Cancer-up-regulated gene 2 protein; CENP-W; cenpw; CENPW_HUMAN; Centromere protein W; chromosome 6 open reading frame 173; CUG2; hypothetical protein LOC387103
Species
Homo sapiens (Human)
Expression Region
1-88
Target Protein Sequence
MALSTIVSQR KQIKRKAPRG FLKRVFKRKK PQLRLEKSGD LLVHLNCLLF VHRLAEESRT NACASKCRVI NKEHVLAAAK VILKKSRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
Gene References into Functions
  1. we suggest that STAT1HDAC4 signaling induces malignant tumor features such as EMT and sphere formation in CUG2overexpressing cancer cells. PMID: 30226605
  2. Report provides evidence that CUG2 induces the EMT in human lung cancer cells via enhancement of TGF-beta signaling and that that TGF-beta signaling is a potential target for CUG2-mediated oncogenesis. PMID: 27974707
  3. It has been proposed that CENP-W may function as a booster of beta-TrCP1 nuclear import to increase the oncogenicity of beta-TrCP1. PMID: 27861801
  4. FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres. PMID: 27284163
  5. CENP-W interacts with hnRNPU and may contribute to kinetochore-microtubule attachment in mitotic cells. PMID: 26881882
  6. CENP-W is a novel kinetochore component that may be involved in the EZH2-mediated silencing machinery. PMID: 26111449
  7. We propose that CENP-W, by influencing proper kinetochore assembly, particularly microtubule docking sites, can confer spindle pole resistance to traction forces exerted by motor proteins during chromosome congression PMID: 25329824
  8. CUG2-mediated antiviral activity can be reduced by ISG15 deficiency in A549 cells, which eventually enhances sensitivity of the cells to vesicular stomatitis virus infection. PMID: 24452380
  9. our results suggest that CUG2 enhances metastasis and drug resistance through STAT1 activation, which eventually contributes to tumor progression PMID: 23917355
  10. CSN5 regulates the stability of the inner kinetochore components CENP-T and CENP-W, providing the first direct link between CSN5 and the mitotic apparatus, highlighting the role of CSN5 as a multifunctional cell cycle regulator. PMID: 23926101
  11. we propose that VSV treatment combined with the selective regulation of genes such as STAT1 and OASL2 will improve therapeutic outcomes for CUG2-overexpressing tumors. PMID: 23306614
  12. Single nucleotide polymorphism in CENPW is associated with the length of reproductive lifespan. PMID: 22131368
  13. B23 may function in the assembly of the kinetochore complex by interacting with CENP-W during interphase PMID: 22002061
  14. The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations. PMID: 21695110
  15. Sp1 together with Sp3 may function as the main regulator of the basal and serum-induced transcription of CENP-W. PMID: 20180024
  16. Overexpression of CUG2 induces apoptosis in SKOV-3 cells. PMID: 20648695
  17. Observational study of gene-disease association. (HuGE Navigator) PMID: 20846217
  18. Observational study of gene-disease association. (HuGE Navigator) PMID: 20546612
  19. data indicate that activation of p38 MAPK and Ras has critical roles in reoviral replication in CUG2-expressing tumor cells. PMID: 20075984
  20. These findings suggest CUG2 is a novel tumor-associated gene that is commonly activated in various human cancers and exhibits high transforming activities; it possibly belongs to a transcription regulator family that is involved in tumor biogenesis. PMID: 17610844
  21. Study demonstrates that CENP-W forms a DNA-binding complex together with the CCAN component CENP-T; this complex directly associates with nucleosomal DNA and with canonical histone H3, but not with CENP-A, in centromeric regions. PMID: 19070575
  22. CUG2 is a new component of the human centromeric complex that is required for proper chromosome segregation during mitosis. PMID: 19533040
  23. Observational study of gene-disease association. (HuGE Navigator) PMID: 19266077

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Subcellular Location
Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Nucleus matrix. Nucleus, nucleolus.
Protein Families
CENP-W/WIP1 family
Tissue Specificity
Highly expressed in ovary, liver, lung and pancreas and to a lower extent in breast and gastrointestinal tract cancers; such as those of the colon, rectum and stomach. Overexpressed in high grade breast invasive tumors. Expressed in many cancer cell types
Database Links

HGNC: 21488

OMIM: 611264

KEGG: hsa:387103

STRING: 9606.ENSP00000357311

UniGene: Hs.486401

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