Recombinant Human ELAV-like protein 4(ELAVL4)

In Stock
Code CSB-EP007595HU
Size $1812
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names ELAVL4
Uniprot No. P26378
Research Area Neuroscience
Alternative Names
ELAV (embryonic lethal abnormal vision Drosophila) like 4; ELAV L4; ELAV like 4; ELAV like protein 4; ELAV-like protein 4; ELAV4_HUMAN; Elavl4; Embryonic lethal abnormal vision Drosophila homolog of like 4; Hu antigen D; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD; PNEM
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-380aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 61.8kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs. Plays a role in the regulation of mRNA stability, alternative splicing and translation. Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA. Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function. By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay. Also binds to the polyadenylated (poly(A)) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding. Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms. Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells. Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA. Binds and stabilizes MSI1 mRNA in neural stem cells. Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression. Protects CDKN1A mRNA from decay by binding to its 3'-UTR. May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization. Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function. Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain. By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth. Binds to BDNF long 3'UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC. By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration. Acts as a stabilizing factor by binding to the 3'UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing. Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex. May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation. Upon glucose stimulation, Ins2 mRNA is released from ELAVL4 and translational inhibition is abolished. Also plays a role in the regulation of alternative splicing. May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and Calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich intronic sequences of CALCA pre-mRNA.
Gene References into Functions
  1. N-myc levels appear to be modulated by the antagonistic interactions of both miR-17, as a negative regulator, and HuD, as a positive regulator in N-myc-amplified neuroblastoma cells. PMID: 28560387
  2. The high titres of HuD in SCLC patients and preferable consistency suggested that HuD may serve as a potential diagnostic criterium for SCLC and may serve as a marker of disease progression. PMID: 28739747
  3. HuD binds to the APP and BACE1 3' UTRs and increases APP and BACE1 levels. PMID: 24857657
  4. study showed HuD is associated with histological grade, iffuse invasion and nodal metastasis in oral squamous cell carcinoma; it regulates expression of VEGF-A, VEGF-D, MMP-2 and MMP-9 PMID: 25278027
  5. Phosphomimetic mutation at position Ser 221 increased nuclear HUD export. PMID: 23978401
  6. Alzheimer's disease brain tissues with elevated neuroserpin protein also showed increased expression of THRbeta1 and HuD PMID: 24036060
  7. It can be involved in the pathogenesis of meningiomas in male patients. PMID: 22965691
  8. found that p97 and its cofactor, UBXD8, destabilize p21, MKP-1, and SIRT1, three established mRNA targets of the RNA-binding protein HuR, by promoting release of HuR from mRNA PMID: 23618873
  9. HuD is a pivotal regulator of insulin translation in pancreatic beta cells. PMID: 22387028
  10. the endogenous function of ELAVL4 in relation to radiation sensitivity might be the regulation of cell proliferation and death PMID: 21491091
  11. Complexes of HuD and Tax epitopes with HLA-A2 are close but imperfect structural mimics: alphabeta T-cell receptor with self antigens is driven to engage HuD in the same traditional binding mode as Tax. PMID: 21282516
  12. alterations of nELAV genes could be involved in the onset and/or progression of a subset of neuroendocrine lung tumors. PMID: 19410329
  13. HuD stabilizes the GAP-43 mRNA through a mechanism that is dependent on the length of the poly(A) tail and involves changes in its affinity for the mRNA PMID: 12034726
  14. HuD expression in neuroblastoma PMID: 12209604
  15. the importance of post-transcriptional mechanisms in regulating AChE expression in differentiating neurons and implicate HuD as a key trans-acting factor in these events PMID: 12468554
  16. Ten HuD-derived peptides immunogenic in HLA-A*0201 restriction demonstrate in vitro binding to the HLA molecule, 8 elicit specific cytotoxic T lymphocytes (CTLs) in a murine model, 2 elicit specific human CTLs, and 1 is naturally processed and presented. PMID: 14512168
  17. Significant T-cell activation occurred in response to 74% of the selected HuD peptides in Hu-positive patients, suggesting a role of cellular immunity during the course of anti-Hu syndrome. PMID: 15735910
  18. potential role for ELAVL4 suggested as a modifier gene for age at onset of Parkinson's disease PMID: 15827745
  19. propose that haploinsufficiency of HuD due to chromosome #1p deletion in neuroblastoma selects for cells that amplify N-myc genes PMID: 16278682
  20. Increased expression of HuD in the transgenic hippocampus is associated with a concomitant increase in acetylcholinesterase transcript levels. PMID: 17234598
  21. HuD plays a role in the post-transcriptional control of GAP-43 mRNA PMID: 17577668
  22. Gene silencing and overexpression of the nELAV member HuD in motoneuronal NSC34 cells indicate that Nova1 mRNA stability and translation are positively and strongly controlled by the nELAV proteins PMID: 18218628
  23. an unregulated expression of HuD disrupts mossy fiber physiology in adult mice in part by altering the expression and phosphorylation of GAP-43 and the amount of free calmodulin available at the synaptic terminal PMID: 18493953
  24. Association with rs967582 in a third cohort further implicates ELAVL4 as a Parkinson disease susceptibility gene. PMID: 18587682
  25. We found no evidence for the presence of HuD-specific T cells in the cerebrospinal fluid of patients with Hu-associated paraneoplastic neurological syndromes. PMID: 19252764

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Subcellular Location Cytoplasm. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cell projection, growth cone.
Protein Families RRM elav family
Tissue Specificity Expressed in pancreatic beta cells (at protein level). Expressed in the brain.
Database Links

HGNC: 3315

OMIM: 168360

KEGG: hsa:1996

STRING: 9606.ENSP00000349594

UniGene: Hs.213050

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