Recombinant Human Ecotropic viral integration site 5 protein homolog (EVI5), partial

Code CSB-YP007867HU
MSDS
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Source Yeast
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Code CSB-EP007867HU
MSDS
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Source E.coli
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Code CSB-EP007867HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007867HU
MSDS
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Source Baculovirus
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Code CSB-MP007867HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EVI5
Uniprot No.
Alternative Names
EVI5; NB4SEcotropic viral integration site 5 protein homolog; EVI-5; Neuroblastoma stage 4S gene protein
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. May play a role in cytokinesis.
Gene References into Functions
  1. The allele and genotype frequencies of rs6680578 and rs11810217 were not significantly different between relapsing-remitting multiple sclerosis (MS) cases and controls. However, in the rs10735781 the GG genotype was significantly associated with MS risk in recessive and co-dominant models. T G T haplotype (rs6680578, rs10735781 and rs11810217 respectively) was associated with MS risk. PMID: 29141798
  2. EVI5 is an useful independent prognostic marker of survival and recurrence in hepatocellular carcinoma PMID: 28765910
  3. EVI5 as a strong candidate disease risk gene in the 1p22.1 multiple sclerosis risk locus PMID: 26433934
  4. Data show that heat shock transcription factor 1/miR-135b/reversion-inducing-cysteine-rich protein with kazal motifs and ecotropic viral integration site 5 axis provides novel insight into the mechanisms of hepatocellular carcinoma metastasis. PMID: 25537516
  5. in HLA-DRB1 positive patients, EVI5 was associated with attacks of greater severity and worse recovery in Multiple sclerosis . PMID: 24130709
  6. cellular functions exhibited by the Evi5 family members PMID: 23669355
  7. An analysis and fine mapping of GFI-EVI5-RPL5-FAM69A locus, genotyping eight Tag-single nucleotide polymorphisms in 732 multiple sclerosis patients and 974 controls from Spain, was performed. PMID: 20087403
  8. Stem cell exhaustion due to Runx1 deficiency is prevented by Evi5 activation in leukemogenesis. PMID: 20008790
  9. a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics PMID: 16033705
  10. Data provide evidence that EVI5 is an essential component of the protein machinery facilitating the final stages of cell septation at the end of mitosis. PMID: 16764853
  11. Evi5 is a Rab11 binding protein and that Evi5 may cooperate with Rab11 to coordinate vesicular trafficking, cytokinesis, and cell cycle control independent of GTPase-activating protein function PMID: 17229837
  12. EVI5 is a risk gene for multiple sclerosis. PMID: 18401352

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Involvement in disease
A chromosomal aberration involving EVI5 is found is a patient with stage 4S neuroblastoma. Translocation t(1;10)(p22;q21) that forms a EVI5-TRNG10 fusion protein. TRNG10 is a probable structural transcript which is normally not translated.
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Associates with the mitotic spindle through anaphase and remains within the midzone and midbody until completion of cytokinesis.
Tissue Specificity
Expressed in various cell lines (at protein level). Expressed in a wide range of tissues including brain and adrenal.
Database Links

HGNC: 3501

OMIM: 602942

KEGG: hsa:7813

STRING: 9606.ENSP00000359356

UniGene: Hs.594434

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