Recombinant Human Endoplasmic reticulum aminopeptidase 1 (ERAP1), partial

Code CSB-YP007760HU
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Source Yeast
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Code CSB-EP007760HU
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Source E.coli
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Code CSB-EP007760HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007760HU
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Source Baculovirus
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Code CSB-MP007760HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERAP1
Uniprot No.
Alternative Names
A LAP; A-LAP; Adipocyte derived leucine aminopeptidase; Adipocyte-derived leucine aminopeptidase; ALAP; Aminopeptidase PILS; Aminopeptidase regulator of TNFR1 shedding; APPILS; ARTS 1; ARTS-1; Arts1; Endoplasmic reticulum aminopeptidase 1; Endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP; ERAAP1; ERAP 1; Erap1; ERAP1_HUMAN; KIAA0525; kiaa0525 protein; PILS AP; PILS-AP; PILSA; PILSAP; Puromycin insensitive leucyl specific aminopeptidase; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; type 1 tumor necrosis factor receptors shedding aminopeptidase regulator; VEGF-induced aminopeptidase
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
Gene References into Functions
  1. There was a significant association between ERAP1 polymorphisms (rs30187 and rs27037) and increased risk of Ankylosing Spondylitis susceptibility. PMID: 30461632
  2. ERAP1 gene rs26653 polymorphism may increase the risk of psoriasis vulgaris in Chinese Han population PMID: 30313118
  3. this study shows the association of ERAP1 single nucleotide polymorphism and its haplotypes with psoriasis vulgaris, and its dependence on the presence or absence of the HLA-C*06:02 allele and age at disease onset PMID: 29183862
  4. ERAP1 and ERAP2 heterodimers have a role in mediating the processing pathway of MHC class I antigens PMID: 27514473
  5. these results indicated that the ERAP gene may play a critical role in Hepatitis C virus chronicity in Chinese Han population PMID: 29037997
  6. this study demonstrates associations of ERAP1 coding variants and domain specific interaction with HLA-C *06 in the early onset psoriasis patients of India PMID: 28867178
  7. To decrease the enzymatic activity of ERAP1. PMID: 28901420
  8. Data demonstrate that HCMV miR-UL112-5p targets ERAP1, thereby inhibiting the processing and presentation of the HCMV pp65495-503 peptide to specific CTLs. PMID: 28746870
  9. these results present the first direct evidence that human disease-associated ERAP1 variants can greatly alter survival, as well as antigen presentation, T-cell repertoire and NK cell responses in vivo in mice PMID: 28814066
  10. The ERAP1 association with ankylosing spondylitis is predominantly attributable to common ERAP1 haplotypes and haplotype combinations. PMID: 28049827
  11. these study reports an association of the ERAP1 SNP rs30187 with the HLA-C*07 allele in inflammatory bowel disease in the Spanish population PMID: 28651467
  12. Study suggests that ERAP1 rs27044 polymorphism may be related to ankylosing spondylitis susceptibility in Chinese Han population. PMID: 29278768
  13. Segregating according to HLAB27 status did not alter the lack of association. rs30187 SNP in ERAP1 does not confer risk of developing ERA or AS in the Asian Indian population. PMID: 28161768
  14. ERAP1 and ERAP2 have significant and distinct effects on the HLA-B*27 peptidome, suggesting that both enzymes largely act as separate entities in vivo. This may explain their different patterns of association with AS. PMID: 28063628
  15. This review focuses on the ambivalent role of HLA-B27 in autoimmunity and viral protection correlating its functions to the quantitative and qualitative effects of ERAP1 and ERAP2 polymorphisms on their enzymatic activity. PMID: 28759104
  16. The ERAP1 gene polymorphism might be a risk factor in the pathogenesis of ankylosing spondylitis and inflammatory bowel disease. In contrast, IL-23R gene polymorphisms may serve a protective role in ankylosing spondylitis and inflammatory bowel disease. PMID: 27458846
  17. Our results suggest that normal levels of ERAP1 reduce the accumulation of aberrant and disulfide-linked forms of HLA-B27 in monocytes, and thus help to maintain the integrity of cell surface HLA-B27 complexes. PMID: 27107845
  18. The alterations in the nature and affinity of HLA-B*51.peptide complexes probably affect T-cell and natural killer cell recognition, providing a sound basis for the joint association of ERAP1 and HLA-B*51 with Behcet's disease. PMID: 28446606
  19. One ERAP1 protein allotype with five non-ancestral amino acids was recessively associated with Behcet's disease. The ERAP1 association was absent in individuals who lacked HLA-B*51. Individuals who carry HLA-B*51 and who are also homozygous for the haplotype had an increased disease odds compared with those with neither risk factor. PMID: 27217550
  20. Electrostatic interactions between domains II and IV in ERAP1 are crucial for driving a conformational change that regulates the structural integrity of the catalytic site. PMID: 28218509
  21. analysis of genotype and haplotype frequencies of four coding, nonsynonymous ERAP1 SNPs, rs26653G > C, rs26618T > C, rs30187C > T, and rs27044C > G, in non-small cell lung carcinoma occurring in two genetically distant populations, Chinese and Poles PMID: 28083613
  22. there is a significant association of ERAP2 with psoriatic arthritis and HLA-B27 negative psoriatic arthritis, while ERAP1 association is restricted only to HLA-B27 positive disease PMID: 28083616
  23. The ERAP1 rs27044/rs30187 haplotype C/T is associated with lower risk of extraspinal disease and systemic inflammation in Nordic AS patients but has no impact on IL-6 or TNF levels. PMID: 27095091
  24. The ERAP1 polymorphisms are associated with the development of ankylosing spondylitis in Europeans and East Asians. [review & meta-analysis] PMID: 27108589
  25. ERAP1 gene rs27434 and rs7711564 polymorphisms may increase the risk of ankylosing spondylitis (AS). PMID: 26617903
  26. Suggest ERAP1 rs27434 SNP is associated with ankylosing spontylitis in Zhejiang Han Chinese population. PMID: 26350268
  27. Silencing or inhibition of endoplasmic reticulum aminopeptidase 1 (ERAP1) suppresses free heavy chain expression and Th17 responses in ankylosing spondylitis. PMID: 26130142
  28. ERAP-1 has a significant influence on the B*51:01 peptidome and its affinity which provides a mechanism for the epistatic association of ERAP-1 and B*51:01 in Behcet's disease. PMID: 26360328
  29. KIR3DL1 interaction with HLA-B27 is altered by ankylosing spondylitis associated ERAP1 and enhanced by MHC class I cross-linking PMID: 26321090
  30. beta2i/MECL-1 and PA28 negatively affect C- and N-terminal cleavage and therefore epitope liberation from the proteasome, whereas ERAP1 destroys the MART-1(26-35) epitope by overtrimming activity PMID: 26399368
  31. epistatic interaction between ERAP1 and the HLA class I alleles HLA-B*27 and HLA-B*40:01 affects susceptibility to ankylosing spondylitits PMID: 25994336
  32. The results reveal the nature of the functional interaction between A*29:02 and ERAP1 and suggest that this enzyme may affect the susceptibility to birdshot chorioretinopathy by altering the A*29:02 peptidome. PMID: 25892735
  33. ERAP1 downregulation is associated with loss of heterozygosity. PMID: 26146606
  34. Genetic variants and haplotypes of ERAP1 are associated with ankylosing spondylitis, psoriasis, and Behcet's disease in people of varying ancestries. PMID: 26002026
  35. CCR1, KLRC4, IL12A-AS1, STAT4, and ERAP1 are bona fide susceptibility genes for Behcet's disease. PMID: 26097239
  36. We concluded that ERAP1 variants are associated with AS in East Asian population, indicating a common pathogenic mechanism for AS in East Asians and Caucasians. PMID: 25817437
  37. Meta-analysis. ERAP1 polymorphisms were associated with AS in Caucasians, but their association with AS in Asians needs further exploration. PMID: 25401226
  38. ERAP1 as being involved in modulating innate responses of human immune cells PMID: 25591727
  39. rs1065407 and rs10050860 of the ERAP1 gene may contribute to the genetic susceptibility of BD by modulating the expression of ERAP1. PMID: 26393469
  40. two of the most consistently discovered disease-associated polymorphisms, namely K528R and Q730E of ERAP1, were analyzed for their effect on the ability of the enzyme to select substrates based on length and to undergo conformational changes. PMID: 26224046
  41. Our data were consistent with an association between ERAP1 and Behcet disease as well as with an epistatic interaction between ERAP1 and HLA-B in the Spanish population. PMID: 25019531
  42. Data demonstrate that aberrant ERAP1 activity and HLA-B27 carriage does not alter endoplasmic reticulum stress levels in ankylosing spondylitis, suggesting that ERAP1 and HLA-B27 may influence disease susceptibility through other mechanisms. PMID: 25354578
  43. Spondyloarthritis-associated ERAP1 polymorphisms affect the level of gene expression in antigen-presenting cells. PMID: 25740711
  44. Studies indicate the critical role of M1 aminopeptidases ERAP1, ERAP2 and NPEPPS in immune-mediated diseases. PMID: 25142031
  45. Peptide handling by HLA-B27 subtypes influences their biological behavior, association with ankylosing spondylitis and susceptibility to ERAP1. PMID: 25187574
  46. data suggest that ERAP1 isoforms may exhibit differential biological properties and inflammatory mediators could play critical roles in modulating ERAP1 expression, leading to altered functional activities of this enzyme. PMID: 25545008
  47. The 3'UTR-1008A>C and 3'UTR-1055A>G polymorphisms of ERAP1 gene are associated with essential hypertension. PMID: 25665737
  48. polymorphic ERAP1 alters protein function predisposing an individual to Ankylosing Spondylitis via its influence on the antigen processing pathway PMID: 25422414
  49. Genetic or pharmacological inhibition of ERAP1 on human tumor cell lines perturbs their ability to engage several classes of inhibitory receptors. PMID: 25592150
  50. The significant alterations in the B*27:05 peptidome and the structural features of the peptides that determine their differential expression in distinct ERAP1 contexts account for the association of the R528K polymorphism with AS PMID: 25469497

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Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Protein Families
Peptidase M1 family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 18173

OMIM: 606832

KEGG: hsa:51752

STRING: 9606.ENSP00000296754

UniGene: Hs.666524

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