Recombinant Human Exportin-5 (XPO5), partial

Code CSB-YP864014HU
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Source Yeast
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Code CSB-EP864014HU
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Source E.coli
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Code CSB-EP864014HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP864014HU
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Source Baculovirus
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Code CSB-MP864014HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XPO5
Uniprot No.
Alternative Names
Exp 5; Exp5; Exportin 5; Exportin-5; Exportin5; FLJ14239; FLJ32057; FLJ45606; KIAA1291; OTTHUMP00000016472; Ran binding protein 21; Ran-binding protein 21; Ranbp 21; Ranbp21; Xpo 5; xpo5; XPO5_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.; Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.; (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
Gene References into Functions
  1. DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers..), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048). PMID: 29683064
  2. XPO5 might have a dual role in promoting or inhibiting tumor growth in different cancer tissue types. PMID: 29699373
  3. overexpression of XPO5 causes dysregulation in the miRNA processing mechanism, resulting in a shift in protein stability via lower ubiquitination and hyper N-linked glycosylation, as well as conferring a growth advantage by suppressing the DNA damage response. PMID: 28881308
  4. data strongly strengthened the notion that phosphorylation of XPO5 by ERK downregulates miRNA expression in clinical samples and is correlated with poor clinical outcomes of HCC patients PMID: 28500493
  5. Aberrant XPO5 expression is important for the maturation of miRNAs and the malignant behavior of melanoma cells. High abundance of XPO5 in melanoma leads to enhanced survival, proliferation and metastasis. PMID: 27556702
  6. XPO5 acts like an oncogene in colorectal cancer by regulating the expression of miRNAs and may be a potential therapeutic target in colorectal cancer PMID: 27553833
  7. one SNP in XPO5 extron (rs2257082) was significantly associated with lead-poisoning; there were no significant association between the other six SNPs and the blood lead levels; polymorphism rs2257082 could be used to distinguish lead-resistant and lead-susceptible populations PMID: 28042866
  8. SNP rs11077 influences the expression of XPO5, and this SNP could also be a potential biomarker for the diagnosis of thyroid cancer, especially in Chinese population PMID: 28383405
  9. findings suggest that XPO5 functions as a potential tumor suppressor in the development and progression of HCC as well as a promising molecular target for HCC therapy PMID: 27000860
  10. The microRNA-related single-nucleotide polymorphisms rs11077 of XPO5 may be independently connected with the prognosis and chemotherapy response of advanced non-small-cell lung cancer patients PMID: 26358254
  11. XPO5 role in microRNA biogenesis PMID: 26976605
  12. NUP93 and exportin 5 interact with the signaling protein SMAD4 and that NUP93 mutations abrogated interaction with SMAD4 PMID: 26878725
  13. Variations in XPO5 rs11077 of Korean patients are significantly associated with their risk of colorectal cancer. PMID: 26147304
  14. Knockdown of exportins 4, 5, and 7 altered thyroid hormone receptor shuttling dynamics, and when exportins 5 and 7 were overexpressed, TR distribution shifted toward the cytosol. PMID: 25911113
  15. eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. PMID: 24209753
  16. data demonstrate that the exportin 5 (XPO5) rs2257082 T variant allele occurs more frequently in primary ovarian insufficiency (POI)patients than in controls, suggesting that this allele may be associated with increased POI risk PMID: 23549446
  17. The study identifies a miR-138-RMND5A-Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells. PMID: 24057215
  18. Dicer, Drosha, and Exportin 5 genes were up-regulated in bladder urothelial carcinoma compared to normal urothelium. Silencing these genes induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma cells. PMID: 22766726
  19. overall survival was significantly longer in multiple myeloma patients with C/C or A/C variant in the XPO5 gene single nucleotide polymorphism site rs11077 PMID: 22539802
  20. Exportin-5 controls Dicer1 expression post-transcriptionally. PMID: 21297638
  21. Data show a requirement for the export receptor Exportin 5 in nuclear export of 60S subunits in human cells, and that Exp5 binds to pre-60S particles in a RanGTP-dependent manner. PMID: 21048991
  22. The restoration of XPO5 functions reverses the impaired export of pre-miRNAs and has tumor-suppressor features. PMID: 20951941
  23. Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. PMID: 12426392
  24. Eukaryotic elongation factor 1A (eEF1A) and tRNA are RanGTP-dependent binding partners of exportin-5 (Exp5). Exp5 stimulates nuclear export of eEF1A and tRNA. PMID: 12426393
  25. exportin-5 has a role in nuclear export of ILF3 (interleukin enhancer binding factor 3) PMID: 14570900
  26. exportin-5 is key to microRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps PMID: 14631048
  27. exportin-5 has a role in exporting JAZ from the cell nucleus PMID: 15254228
  28. Each of the dsRBDs in ADAR1 interacts with the export factor exportin-5. PMID: 19124606
  29. 2.9 angstrom structure of the pre-miRNA nuclear export machinery formed by pre-miRNA complexed with Exp-5 and RanGTP; RNA recognition by Exp-5:RanGTP does not depend on RNA sequence, implying that Exp-5:RanGTP can recognize a variety of pre-miRNAs PMID: 19965479

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Subcellular Location
Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm.
Protein Families
Exportin family
Tissue Specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 17675

OMIM: 607845

KEGG: hsa:57510

STRING: 9606.ENSP00000265351

UniGene: Hs.203206

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