Name: Recombinant Human HLA class II histocompatibility antigen, DR beta 4 chain (HLA-DRB4), partial
Brand: CUSABIO
SKU: 320271HU

Product Details

Purity

>85% (SDS-PAGE)

Target Names

HLA-DRB4

Uniprot No.

P13762

Alternative Names

DR 4; DR beta 4 chain; DR4; DRB1 transplantation antigen ; DRB4; DRB4_HUMAN; HLA class II histocompatibility antigen; HLA class II histocompatibility antigen DR beta 4 chain ; HLA-DRB4; Human leucocyte antigen DRB4 ; Leukocyte antigen ; Major histocompatibility complex class II DR beta 4 ; MHC class II antigen DRB4; MHC class II antigen HLA DR beta ; MHC class2 antigen ; MHC HLA DR-beta chain

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

We are trying to develop a multiplexing assay on Luminex to detect the HLA Class I and II antibodies in individuals.
We are looking for manufacturer who can provide us human derived recombined HLA class I and II antigens, checked on your website
and find some Locus antigens are available with you. For the panel designing we need antigens of locu DRB4.
Could you please provide some idea about it?

As to your request for the antigen of DRB4, please check detailed information as follows:
We can provide all of these antigens expressed by our four expression systems: E.coli, Yeast, Baculovirus and Mammalian Cell, please check and confirm which expression system you need.
DRB4
Recombinant Human HLA class II histocompatibility antigen, DR beta 4 chain(HLA-DRB4),partial
CSB-YP320271HU >> Yeast
CSB-EP320271HU >> E.coli
CSB-BP320271HU >> Baculovirus
CSB-MP320271HU >> Mammalian cell
Expression Region:30-227aa; Provide the complete extracellular domain.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows: YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

GDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSK

Target Background

Function

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Gene References into Functions

We developed an human cardiac alpha-myosin -induced myocarditis model in human HLA-DR4 transgenic mice that lack all mouse MHCII genes. PMID: 28431892
Strong association of nontumor anti-LGI1 encephalitis with HLA-DRB4. PMID: 28026046
HLA-DR4 is a susceptibility factor for the development of AIH. Impaired suppressive function of Tregs and reduced PD-1 expression may result in spontaneous activation of key immune cell subsets, such as antigen-presenting cells and CD8(+) T effectors, facilitating the induction of AIH and persistent liver damage. PMID: 27414259
HLA-DRB4 affects type 1 diabetes risk and islet autoantibodies. PMID: 26740600
The study identifies a region of focus for B and T cell responses to IA-2 in HLA-DR4 diabetic patients that may explain HLA associations of IA-2 autoantibodies. PMID: 25225671
HLA-DR4 codes for susceptibility to RF+ polyarticular JIA with a six-fold risk. PMID: 24618287
The HLA- DR4 gene was clearly associated with susceptibility to rheumatoid arthritis. PMID: 23537298
Data indicate that invariant NKT (iNKT) cell-mediated cytokine secretion in staphylococcal enterotoxin B (SEB)-challenged HLA-DR4-transgenic mice was CD1d-independent. PMID: 22041925
Elevated frequencies of HLA-DR4 and HLA-DR5 alleles were found in patients with idiopathic dilated cardiomyopathy compared with controls. PMID: 21556773
DRB4*01:08 is a novel HLA-DRB4 allele PMID: 21410658
HLA-DR14/DR7/DQ5 alleles significantly increase the risk for toxic shock syndrome, regardless of individual variations in T cell receptor variable region repertoires. PMID: 21282506
IA2 positivity was associated with HLA-DR4/X and HLA-DR3/4 positivity, and hypothyroidism was linked to HLA-DR4/4. More females carried the HLA-DR4/4 genotype or were thyroid antibody positive. PMID: 20371654
Our results revealed HLA-DRB1*04 as predisposing factor in papillary thyroid carcinoma in Iranian population. PMID: 20164547
Complete coding sequence of the HLA alleles DRB4*0103101 and DRB4*01033 PMID: 11972878
DRB4 was increased in males with childhood ALL compared to age- and sex-matched controls) and female patients. HLA-DRB4 is over-represented in high-risk patients. The HLA system may be a component of genetic leukemia susceptibility in male children only. PMID: 12008082
Comparison of HLA-DR4-associated peptides in neuroendocrine cells with those identified in lymphoblastoid B cells (LCLs) suggests that intracellular pathways allowing HLA-DR endogenous peptide processing are more efficient in endocrine cells than in LCLs. PMID: 12391221
A novel allele of HLA-DRB4 was found. PMID: 12859598
differential expression of genes encoded within the RA-associated HLA-DR4 superhaplotype and within the neutral DR7 and DR9 superhaplotypes. PMID: 14558083
In patients with autoimmune thyroiditis the HLA-DR11 frequency was higher than control values, while in patients with autoimmune polyglandular syndrome type II, the HLA-DR3 frequency was found to be higher. PMID: 15046556
Interaction between the HLA-DRB4 and CTLA-4 genes determines the thyroid function of TPO-positive goitrous Japanese Hashimoto's thyroiditis patients. PMID: 15055474
No definite association was found between HLA-DR alleles and the risk of psoriasis or psoriatic arthritis. PMID: 15513680
HIP is an islet protein naturally processed and presented by HLA-DR4 molecules PMID: 15721314
Genetic variation associated with type 1 diabetes in a Czech Republic population with childhood onset. PMID: 16629714
a diabetic intrauterine environment interacts with gene(s) marked by the type 1 diabetes susceptibility HLA DR4 alleles to increase fetal growth PMID: 17310371
The shared epitope assessed on all HLA-DRB1 serotypic backgrounds except DR1 was associated with RA susceptibility. The presence of the shared epitope on DR4 is associated with greater RA susceptibility and certain disease-activity measures. PMID: 17491100
findings indicate that HLA-DRB4 is a genetic risk factor for the development of Churg-Strauss syndrome and increases the likelihood of development of vasculitic manifestations of the disease. PMID: 17763415
DRB1*04 may be a protective factor in paucibacillary leprosy. PMID: 18053473
A model of mixed connective tissue disease has been developed in mice that express the transgene HLA-DRA*0101/DRB1*0401 in a fusion protein with U1-70-kDa ribonucleoprotein autoantigen. PMID: 18523312
Thirty-eight percent of the B burgdorferi-infected DR4+/+CD28(-/-)MHCII(-/-) mice, but none of the B burgdorferi-infected CD28(-/-)MHCII(-/-) mice, remained arthritic post-antibiotic treatment PMID: 19035513
associated with antiphospholipid syndrome PMID: 19052923
Significant transmission disequilibrium for HLA-DR4 was seen (odds ratio, 4.67; 95% confidence interval, 1.34-16.24; P = .008) for transmissions from maternal grandparents to mothers of probands. PMID: 19487610

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Note=The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

Protein Families

MHC class II family

Database Links

HGNC: 4952

KEGG: hsa:3126

UniGene: Hs.534322

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Code	CSB-YP320271HU
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP320271HU
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP320271HU-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP320271HU
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP320271HU
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human HLA class II histocompatibility antigen, DR beta 4 chain (HLA-DRB4), partial

Product Details

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