Recombinant Human Inward rectifier potassium channel 18 (KCNJ18), partial

Code CSB-YP488117HU
MSDS
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Source Yeast
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Code CSB-EP488117HU
MSDS
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Source E.coli
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Code CSB-EP488117HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP488117HU
MSDS
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Source Baculovirus
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Code CSB-MP488117HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KCNJ18
Uniprot No.
Alternative Names
KCNJ18; Inward rectifier potassium channel 18; Inward rectifier K(+) channel Kir2.6; Potassium channel; inwardly rectifying subfamily J member 18
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Gene References into Functions
  1. Study describes the functional consequences of a single amino acid change in Kir2.6 channel. The D252N mutation down-regulates the Kir2.6 activity, decreasing the K+ current density ( approximately 34%) when compared to the wildtype channel; whereas the mutation R386C shows no significant changes from wildtype. PMID: 28131627
  2. A novel KCNJ18 mutation, G169R, identified in the hypokalemic periodic paralysis patient demonstrated a significant functional defect in vitro. PMID: 27178871
  3. KCNJ18 alterations are seldom pathogenic PMID: 25882930
  4. 3.1% of thyrotoxic periodic paralysis cases had KCNJ18 gene mutations in mainland Chinese patients. Patients with KCNJ18 mutation had shorter attack duration, higher prevalence of muscle soreness and weakness recurrence than patients without the mutation. PMID: 25885757
  5. TPP and SPP have the same susceptible gene variant rs623011 and may share the pathogenic mechanism of reduced Kir current in skeletal muscle independent of thyroid hormone. PMID: 22910584
  6. suggest that decreased outward K(+) current from hypofunction of Kir2.6 predisposes the sarcolemma to hypokalemia-induced paradoxical depolarization during attacks, which in turn leads to Na(+) channel inactivation and inexcitability of muscles PMID: 21665951
  7. Kir2.6 regulates the surface expression of Kir2.x inward rectifier potassium channels. PMID: 21209095
  8. While sequencing candidate genes, we identified a previously unreported gene (not present in human sequence databases) that encodes an inwardly rectifying potassium (Kir) channel, Kir2.6. PMID: 20074522

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Involvement in disease
Thyrotoxic periodic paralysis 2 (TTPP2)
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ12 subfamily
Tissue Specificity
Specifically expressed in skeletal muscle.
Database Links

HGNC: 39080

OMIM: 613236

KEGG: hsa:100134444

UniGene: Hs.200629

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