Recombinant Human Kelch domain-containing protein 1 (KLHDC1)

Code CSB-YP847651HU
MSDS
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Source Yeast
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Code CSB-EP847651HU
MSDS
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Source E.coli
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Code CSB-EP847651HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847651HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP847651HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KLHDC1
Uniprot No.
Alternative Names
KLHDC1; MSTP025Kelch domain-containing protein 1
Species
Homo sapiens (Human)
Expression Region
1-406
Target Protein Sequence
MADSQLFCVA EERSGHCAVV DGNFLYVWGG YVSIEDNEVY LPNDEIWTYD IDSGLWRMHL MEGELPASMS GSCGACINGK LYIFGGYDDK GYSNRLYFVN LRTRDETYIW EKITDFEGQP PTPRDKLSCW VYKDRLIYFG GYGCRRHSEL QDCFDVHDAS WEEQIFWGWH NDVHIFDTKT QTWFQPEIKG GVPPQPRAAH TCAVLGNKGY IFGGRVLQTR MNDLHYLNLD TWTWSGRITI NGESPKHRSW HTLTPIADDK LFLCGGLSAD NIPLSDGWIH NVTTNCWKQL THLPKTRPRL WHTACLGKEN EIMVFGGSKD DLLALDTGHC NDLLIFQTQP YSLLRSCLDC IGKNSIMLES QISLLPPKLL QQVLKKITFW AAANHREEQR VQKEETENKY QWISSN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-recognition component of a Cul5-RING (CRL5) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL5(KLHDC1) complex mediates ubiquitination and degradation of truncated SELENOS selenoprotein produced by failed UGA/Sec decoding, which ends with a glycine.
Gene References into Functions
  1. The crystal structures of the apo form of the mutated Keap1 Kelch domain (1.98 A resolution) and of the complex with an Nrf2-derived peptide obtained by soaking (2.20 A resolution) are reported. PMID: 23722832
  2. Blocking CCR4 and CCR10 simultaneously, or their ligands, should be beneficial in the treatment of T-cell-mediated skin diseases. PMID: 18095942
  3. Data show that KLHDC1 and KLHDC2 have differential localization and activity in cultured mammalian cells. PMID: 16964437
Subcellular Location
Cytoplasm, cytosol.
Tissue Specificity
Widely expressed, with high levels in skeletal muscle, pancreas and liver. Undetectable in peripheral blood leukocytes.
Database Links

HGNC: 19836

OMIM: 611281

KEGG: hsa:122773

STRING: 9606.ENSP00000352282

UniGene: Hs.509258

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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