Recombinant Human Low-density lipoprotein receptor-related protein 2(LRP2),partial

Code CSB-YP013096HU
Size US$1298
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names LRP2
Uniprot No. P98164
Research Area Immunology
Alternative Names Calcium sensor protein; DBS; Glycoprotein 330; gp330; Heymann nephritis antigen homolog; Low-density lipoprotein receptor-related protein 2; LRP-2; Lrp2; LRP2_HUMAN; Megalin
Species Homo sapiens (Human)
Source Yeast
Expression Region 1186-1389aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 24.2kDa
Protein Length Extracellular Domain
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Multiligand endocytic receptor (By similarity). Acts together with CUBN to mediate endocytosis of high-density lipoproteins (By similarity). Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B (By similarity). In the kidney, mediates the tubular uptake and clearance of leptin (By similarity). Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium (By similarity). Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight (By similarity). Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells (By similarity). Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP (By similarity). Mediates renal uptake of metallothionein-bound heavy metals
Gene References into Functions
  1. megalin is critical for mitochondrial biology; mitochondrial intracrine signaling is a continuum of the retrograde early endosome-to-Golgi-Rab32 pathway and defects in this pathway may underlie disease processes in many systems. PMID: 29916093
  2. patients with OCRL-1 mutations or type 1 Dent disease showed abnormally low levels of urinary A-megalin PMID: 27766457
  3. An Emerging Role for Megalin as a Regulator of Protein Leak in Acute Lung Injury. PMID: 29090957
  4. Review of LRP2 function. LRP2 functions are crucial for developmental processes in a number of tissues, including the brain, the eye, and the heart, and defects in this receptor pathway are the cause of devastating congenital diseases in humans. PMID: 26872844
  5. A novel LRP2 missense variant rs17848169 (N2632D) was found to be associated with lower risk for T2D-ESRD in this population. PMID: 27197912
  6. we discovered one novel locus (LRP2; most significant single nucleotide polymorphism rs12988804) that reached genome-wide significance in predicting relapse risk (HR=2.18, p=3.30x10(-8)). PMID: 28739605
  7. miR-148b directly down-regulates renal megalin expression. PMID: 28331063
  8. Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID: 28289043
  9. The studies suggest that impaired endocytosis of megalin/cubilin ligands, hemoglobin and albumin, rather than heme toxicity, may be the cause of tubular proteinuria in sickle cell disease patients. PMID: 28356267
  10. VDR and MEGALIN gene variations can alter age-related cognitive trajectories differentially between men and women among African American urban adults, specifically in global mental status and domains of verbal fluency, visual/working memory, and executive function. PMID: 28446629
  11. the main role for placental megalin is not to mediate uptake of nutrients from the maternal bloodstream; results point toward novel and complex functions for megalin in the cytotrophoblasts. PMID: 27798286
  12. specific miRNA-146a regulation may contribute to Alzheimer's disease by downregulating the Lrp2/Akt pathway PMID: 27241555
  13. abundances of megalin and Dab2 (p = 0.046) were reduced in infected placentas from women with LBW deliveries PMID: 27072056
  14. homozygous Asp3779Asn and a hemizygous Ile262Met mutations in the LRP2 and TSPYL2 genes, respectively, in a Pakistani family with two boys affected with mild nonsyndromic intellectual disability PMID: 26529358
  15. The megalin expression appears to vary inversely with gestational age with the greatest expression noted in the most premature samples. Age-dependent differences in placental megalin may therefore influence fetal exposure. PMID: 25304941
  16. we are the first to identify the association between LRP2 and gout in a Chinese population and to confirm this association in Asians. PMID: 26147675
  17. Two novel LRP2 mutations, a homozygous nonsense mutation and a missense mutation in two unrealted families with Donnai-Barrow syndrome. PMID: 25682901
  18. Loss of LRP2 is associated with buphthalmos PMID: 26439398
  19. melanoma cell expression of LRP2/megalin significantly decreases melanoma cell proliferation and survival rates. PMID: 25585665
  20. levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients PMID: 25502002
  21. A new mutation in LRP2 causes a predominantly ocular phenotype suggestive of Stickler syndrome. PMID: 23992033
  22. LRP2 sequencing reveals multiple rare variants associated with urinary trefoil factor-3 PMID: 24876117
  23. Serum uric acid-related gene LRP2 is not involved in gout susceptibility. PMID: 24366390
  24. Transgenic/knock-out megalin-deficient mice develop anxiety behavior and impaired learning, as described in Alzheimer's disease. PMID: 24254699
  25. Association of the T-allele of a single nucleotide polymorphism in LRP2 with gout risk in the Maori and Pacific subjects was consistent with this allele increasing serum urate in Japanese individuals PMID: 24286387
  26. LOS treatment decreased microalbuminuria induced by Cd apparently through a cubilin receptor-dependent mechanism but independent of megalin. PMID: 24093454
  27. Data suggest that endodermal layer of yolk sac and syncytiotrophoblast/cytotrophoblast cells of placental villi express megalin mRNA/protein; expression of megalin protein (but not mRNA) is up-regulated as gestation/placentation progresses. PMID: 23978537
  28. This review explores current evidence linking megalin expression and function to the development, diagnosis, and progression of acute kidney injury --{REVIEW} PMID: 24197071
  29. Megalin and Dab2 were expressed in prostate and colon epithelial cells, which was markedly enhanced following treatment with retinoic acid PMID: 23909735
  30. The hypothalamic clusterin-low-density lipoprotein receptor-related protein-2 axis is a novel anorexigenic signalling pathway. PMID: 23673647
  31. results suggested that GSTT1 wild genotype and C-allele of megalin gene rs2228171 SNPs might be risk factors for cisplatin-induced ototoxicity PMID: 23274376
  32. Using NMR titration data in HADDOCK, we have generated a three-dimensional model describing the complex between megalin and gentamicin. PMID: 23275343
  33. Expression of megalin and cubilin is decreased during experimental endotoxemia, which may contribute to an increase in urine levels of albumin during acute renal failure. PMID: 22437417
  34. analysis of rare disease variants in LRP2, a gene linked and associated with autism spectrum disorders PMID: 22578327
  35. This study confirmed that LRP2 rs2544390 C/T at intron 1 was associated with serum uric acid levels among Japanese males with SLC22A12 258WW, SLC2A9 rs11722228C allele, ABCG2 126QQ and 141Q allele. PMID: 22565184
  36. A total of 330 Chinese female-offspring nuclear families with 1088 individuals and 400 Chinese male-offspring nuclear families with 1215 individuals were genotyped at six tag single nucleotide polymorphisms of the LRP2 gene. PMID: 22174918
  37. Sex-specific VDR and Megalin gene variations can modify age-related cognitive decline among US adults. PMID: 22170372
  38. megalin and cubilin are involved in the metabolism of vitamin D by reabsorbing vitamin D binding protein; dysfunction of these receptors is likely to be associated with the development of vitamin D deficiency in patients with chronic kidney disease PMID: 21595846
  39. allele (A) of the rs3755166 polymorphism within LRP2 gene may contribute to Alzheimer's disease risk in the Chinese Han Population PMID: 20971101
  40. in anagen VI hair follicles megalin was found in all keratinocytes of the distal region PMID: 21104416
  41. Data show that MT-I + II and megalin are significantly altered in CNS lymphoma relative to controls. PMID: 20038220
  42. No defect in the trafficking or function of megalin upon OCRL1 knockdown. PMID: 19940034
  43. Megalin and cubilin: multifunctional endocytic receptors. A review. PMID: 11994745
  44. This study reveals that LRP2 is a major autoantigen in rheumatoid arthritis and probably drives the production of anti-LRP2 autoantibodies, which may play pathological roles by inhibiting the reabsorbing function of LRP2. PMID: 12723989
  45. megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID: 14657389
  46. a binding affinity of disabled homolog 2 mitogen-responsive phosphoprotein interaction domain for megalin CT of K(D) = 2.6 x 10(-7) +/- 5.3 x 10(-8) PMID: 15134832
  47. megalin endocytosed NGAL by a mechanism completely blocked by an antibody against megalin PMID: 15670845
  48. Further studies on the intracellular molecular signalling associated with megalin-mediated metabolic pathways may lead to the development of novel strategies for the treatment of nephropathies related to diabetes and metabolic syndrome. (Review) PMID: 16174284
  49. This review focuses on the involvement of megalin during embryonic development and its interactions with the developmental morphogen sonic hedgehog. PMID: 16828734
  50. Results show that the PPPSP motif and GSK3 activity are critical to allow megalin phosphorylation and also negatively regulate the receptor's recycling. PMID: 17555532
  51. Study mapped Donnai-Barrow syndrome to chromosome 2q23.3-31.1 and identified LRP2 mutations in six families with Donnai-Barrow syndrome and one family with facio-oculo-acoustico-renal syndrome. PMID: 17632512
  52. This review summarizes recent data on the biological function of megalin and focuses on its implication in embryonic nutrition and central nervous system malformations. PMID: 17979745
  53. These results suggest that Dab2 is a ligand dependent bi-directional regulator of ERK1,2 activity. PMID: 18070591
  54. confirm the association between LRP2 and levels of T-Cho and LDL-C in human plasma PMID: 18174661
  55. light chain endocytosis is predominantly mediated by the megalin-cubilin tandem endocytic receptor and identify endocytosis as a key step in light chain cytotoxicity. PMID: 18448595
  56. Polymorphisms within the megalin gene for associations with prostate cancer risk and outcomes. PMID: 18559602
  57. Interaction of the A2 domain of F8 with LRP2 follows the general mode, requires dissociation of factor VIII from von Willebrand factor, and is activation sensitive. PMID: 18685438
  58. results show different expression patterns of megalin and cubulin in calculous gallbladders and acalculous gallbladders suggesting an association with gallstone formation and implying a putative role of the two proteins in cholesterol endocytosis PMID: 18791690

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Involvement in disease Donnai-Barrow syndrome (DBS)
Subcellular Location Apical cell membrane, Single-pass type I membrane protein, Endosome lumen, Membrane, coated pit, Cell projection, dendrite, Cell projection, axon
Protein Families LDLR family
Tissue Specificity Expressed in first and third trimester cytotrophoblasts in the placenta (at protein level) (PubMed:27798286). Absorptive epithelia, including renal proximal tubules.
Database Links

HGNC: 6694

OMIM: 222448

KEGG: hsa:4036

STRING: 9606.ENSP00000263816

UniGene: Hs.657729

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