Recombinant Human Meiotic recombination protein DMC1/LIM15 homolog (DMC1)

Code CSB-YP617920HU
MSDS
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Source Yeast
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Code CSB-EP617920HU
MSDS
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Source E.coli
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Code CSB-EP617920HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP617920HU
MSDS
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Source Baculovirus
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Code CSB-MP617920HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DMC1
Uniprot No.
Alternative Names
Disrupted meiotic cDNA 1 homolog ; Disrupted meiotic cDNA 1; yeast; homolog of; dJ199H16.1; DMC 1; DMC1; DMC1 dosage suppressor of mck1 homolog; DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast); DMC1 homologue ; DMC1_HUMAN; DMC1H; DNA meiotic recombinase 1; HsLim15; LIM15; Meiotic recombination protein DMC1/LIM15 homolog; MGC150472; MGC150473
Species
Homo sapiens (Human)
Expression Region
1-340
Target Protein Sequence
MKEDQVVAEE PGFQDEEESL FQDIDLLQKH GINVADIKKL KSVGICTIKG IQMTTRRALC NVKGLSEAKV DKIKEAANKL IEPGFLTAFE YSEKRKMVFH ITTGSQEFDK LLGGGIESMA ITEAFGEFRT GKTQLSHTLC VTAQLPGAGG YPGGKIIFID TENTFRPDRL RDIADRFNVD HDAVLDNVLY ARAYTSEHQM ELLDYVAAKF HEEAGIFKLL IIDSIMALFR VDFSGRGELA ERQQKLAQML SRLQKISEEY NVAVFVTNQM TADPGATMTF QADPKKPIGG HILAHASTTR ISLRKGRGEL RIAKIYDSPE MPENEATFAI TAGGIGDAKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Gene References into Functions
  1. Data indicate that ahomologous pairing by DMC1 protein or RAD51 recombinase in chromatin containing nucleosome-depleted double-stranded DNA (dsDNA) regions. PMID: 27052786
  2. BRCA2 protein stimulates DMC1-mediated DNA strand exchange between RPA-ssDNA complexes and duplex DNA, thus identifying BRCA2 as a mediator of DMC1 recombination function. PMID: 26976601
  3. Dmc1 can reject divergent DNA sequences while bypassing a few mismatches in the DNA sequence. PMID: 26709229
  4. In contrast to RAD51, stabilization of the presynaptic filament via ATP hydrolysis attenuation is insufficient for enhancement of the DMC1-catalyzed recombination reaction. PMID: 26088134
  5. truncated DMC1 octamers further stack with alternate polarity into a filament PMID: 23545642
  6. results suggested mutations in the coding sequence of DMC1 are not associated with premature ovarian failure in Chinese women PMID: 23265958
  7. conserved lysine in the Walker A motif of hDMC1 is critical for ATP binding. PMID: 23182424
  8. The results suggested that PSF may function as an activator for the meiosis-specific recombinase DMC1. PMID: 22156371
  9. D-loops in a human DMC1-driven reaction are substantially more resistant to dissociation by branch-migration proteins such as RAD54 than those formed by RAD51. PMID: 21151113
  10. the Dmc1 filament formed on single-stranded DNA has a mass per unit length expected from approximately 6.5 subunits per turn PMID: 20600108
  11. p53 might be involved in homologous recombination and/or checkpoint function by directly binding to DMC1 protein to repress genomic instability in meiotic germ cells PMID: 14764457
  12. The monomeric structure of the Dmc1 protein closely resembled those of the human and archaeal Rad51 proteins. We found another hydrogen bonding interaction at the polymer interface. PMID: 15125839
  13. hDmc1-mediated DNA recombination initiates through the nucleation of hDmc1 onto ssDNA to form a helical nucleoprotein filament PMID: 15164066
  14. N-terminal domain of DMC1 is required for the formation of the octamer, which may support the proper DNA binding activity of the DMC1 protein. PMID: 15917243
  15. activation of hDmc1 is mediated through conformational changes induced by free Ca2+ ion binding to a protein site that is distinct from the Mg2+.ATP-binding center PMID: 15917244
  16. BRCA2 binds the meiosis-specific recombinase DMC1 and define the primary DMC1 interaction site to a 26 amino-acid region PMID: 17541404
  17. Hop2/Mnd1 greatly stimulates Dmc1 to promote synaptic complex formation on long duplex DNAs PMID: 17639081
  18. Data show that DMC1 mutations may be one explanation for premature ovarian failure. PMID: 18166824
  19. The interaction of DMC1 and the homologue of the large subunit of CAF-1 is reported. PMID: 18355319
  20. Results indicate that Rad51 and Dmc1 filaments are essentially identical with respect to several structural parameters, including persistence length, helical pitch, filament diameter, DNA base pairs per helical turn and helical handedness. PMID: 18535008
  21. Biochemical analyses revealed that the human DMC1-M200V variant had reduced stability, and was moderately defective in catalyzing in vitro recombination reactions. PMID: 18566005
  22. Reversibility, equilibration, and fidelity of strand exchange reaction between short oligonucleotides promoted by RecA protein from escherichia coli and human Rad51 and Dmc1 proteins. PMID: 19004837
  23. DMC1-I37N polymorphism may be a source of improper meiotic recombination, causing meiotic defects in humans PMID: 19076215

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Subcellular Location
Nucleus. Chromosome.
Protein Families
RecA family, DMC1 subfamily
Database Links

HGNC: 2927

OMIM: 602721

KEGG: hsa:11144

STRING: 9606.ENSP00000216024

UniGene: Hs.339396

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