Recombinant Human Methyltransferase-like protein 21B (METTL21B)

Code CSB-YP836184HU
MSDS
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Source Yeast
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Code CSB-EP836184HU
MSDS
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Source E.coli
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Code CSB-EP836184HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836184HU
MSDS
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Source Baculovirus
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Code CSB-MP836184HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EEF1AKMT3
Uniprot No.
Alternative Names
FAM119B; Hepatocellular carcinoma-associated antigen 557a; Methyltransferase like 21B; Methyltransferase-like protein 21B; mettl21b; MT21B_HUMAN; Protein-lysine methyltransferase METTL21B
Species
Homo sapiens (Human)
Expression Region
1-226
Target Protein Sequence
MADPGPDPES ESESVFPREV GLFADSYSEK SQFCFCGHVL TITQNFGSRL GVAARVWDAA LSLCNYFESQ NVDFRGKKVI ELGAGTGIVG ILAALQGGDV TITDLPLALE QIQGNVQANV PAGGQAQVRA LSWGIDHHVF PANYDLVLGA DIVYLEPTFP LLLGTLQHLC RPHGTIYLAS KMRKEHGTES FFQHLLPQHF QLELAQRDED ENVNIYRARH REPRPA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protein-lysine methyltransferase that selectively mono-, di- and trimethylates 'Lys-165' of the translation elongation factors EEF1A1 and EEF1A2 in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.
Gene References into Functions
  1. Results have shown that METTL21B is a protein methyltransferase that methylates both isoforms of translation elongation factor eEF1A at lysine 165. Also, further data suggests that METTL21B has an evolutionarily important role for the methylation of lysine 165 in eEF1A. PMID: 28663172
  2. The present study identifies METTL21B as the enzyme responsible for methylation of eEF1A on Lys-165 and shows that this modification is dynamic, inducible and likely of regulatory importance. PMID: 28108655
  3. The METTL21B protein is here suggested to be a protein methyltransferase, as it is shown to belong to a family of 10 human methyltransferases, some of which are shown to have lysine specific protein methyltransferase activity. PMID: 22948820
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Methyltransferase superfamily, METTL21 family
Database Links

HGNC: 24936

OMIM: 615258

KEGG: hsa:25895

UniGene: Hs.632720

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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