Recombinant Human Serine/threonine-protein phosphatase 1 regulatory subunit 10 (PPP1R10), partial

Code CSB-YP836284HU
MSDS
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Source Yeast
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Code CSB-EP836284HU
MSDS
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Source E.coli
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Code CSB-EP836284HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP836284HU
MSDS
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Source Baculovirus
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Code CSB-MP836284HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PPP1R10
Uniprot No.
Alternative Names
CAT 53; CAT53; FB 19; FB19; FB19 protein; HLA-C associated transcript 53; MHC class I region proline rich protein CAT53; MHC class I region proline-rich protein CAT53; p99; Phosphatase 1 nuclear targeting subunit; Phosphatase nuclear targeting subunit; PNUTS; PP1 binding protein of 114 kDa; PP1 nuclear targeting subunit; PP1-binding protein of 114 kDa; PP1R10; PP1RA_HUMAN; ppp1r10; Protein FB19; Protein phosphatase 1 nuclear targeting subunit; Protein phosphatase 1 regulatory (inhibitor) subunit 10; Protein phosphatase 1 regulatory subunit 10; R111; Serine/threonine protein phosphatase 1 regulatory subunit 10; Serine/threonine-protein phosphatase 1 regulatory subunit 10
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.
Gene References into Functions
  1. The work demonstrates that PP1/PNUTS stabilizes chromatin-bound MYC in proliferating cells. PMID: 30158517
  2. A -31 G to A "hot zone" putative functional noncoding variant of PPP1R10 was found in an AML patient. It perturbs the binding activities of E2F1, E2F4, ZBTB33, and TBP. SRSF1 and PPP1R10 genes are interacting partners in a protein-protein interaction network. PMID: 29764005
  3. PNUTS is a bifunctional RNA encoding both PNUTS mRNA and lncRNA-PNUTS, each eliciting distinct biological functions. While PNUTS mRNA is ubiquitously expressed, lncRNA-PNUTS appears to be tightly regulated dependent on the status of hnRNP E1 and tumour context. PMID: 28825698
  4. Biophysical analysis of PNUTS suggests an extended transcription factor TFIIS-like fold. PMID: 27591855
  5. PNUTS was a valid target of miR-383 and was involved in the inhibition of gammaH2AX. PMID: 24462707
  6. PNUTS inhibits the PP1-mediated dephosphorylation of critical substrates, especially retinoblastoma protein, by blocking their binding sites on PP1. PMID: 24591642
  7. our studies reveal PNUTS as a novel PTEN regulator and a likely oncogene. PMID: 23117887
  8. PNUTS is identified as a new and integral component of the DNA damage response involved in DNA repair. PMID: 20890310
  9. stimulation of PP1 activity via siRNA mediated knockdown of its interacting protein PNUTS (Phosphatase Nuclear Targeting Subunit) leads to Rb dephosphorylation and apoptosis in cancer cells PMID: 20372802
  10. PNUTS has a role in protein kinase A-regulated targeting of PP1 to specific RNA-associated complexes in the nucleus PMID: 12574161
  11. PNUTS, encoded by CAT 53 on 6p21.3, may have a role in the progression of AD. PMID: 15894402
  12. results illustrate an involvement of the PNUTS:PP1 holoenzyme in chromosome decondensation in vitro and argue that PNUTS functions as a PP1-targeting subunit in this process PMID: 15907195
  13. Findings suggest that PNUTS may play an important role in controlling cell death in response to cellular stresses such as hypoxia through the post-translational modification of p53 and MDM2. PMID: 17318220
  14. reduced expression of PNUTS leads to activation of Rb-phosphatase and caspase-mediated apoptosis PMID: 18360108
  15. the coordinated spatial and temporal regulation of LCP1 and PNUTS may be a novel mechanism to control the expression of genes that are critical for certain physiological and pathological processes. PMID: 19293638
  16. no evidence found of association between a CAT53 polymorphism and Alzheimer's Disease (AD), but found a significant negative association of the C282Y HFE mutation with AD PMID: 19429178

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Database Links

HGNC: 9284

OMIM: 603771

KEGG: hsa:5514

STRING: 9606.ENSP00000365694

UniGene: Hs.106019

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