Recombinant Human Sperm protein associated with the nucleus on the X chromosome B/F (SPANXB1)

Code CSB-YP865126HU
MSDS
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Source Yeast
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Code CSB-EP865126HU
MSDS
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Source E.coli
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Code CSB-EP865126HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865126HU
MSDS
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Source Baculovirus
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Code CSB-MP865126HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPANXB1
Uniprot No.
Alternative Names
SPANXB1; SPANXB; SPANXB2; SPANXF1; Sperm protein associated with the nucleus on the X chromosome B1; Cancer/testis antigen 11.2; CT11.2; Nuclear-associated protein SPAN-Xb; SPANX-B; SPANX family member B1; SPANX family member F1
Species
Homo sapiens (Human)
Expression Region
1-103
Target Protein Sequence
MGQQSSVRRL KRSVPCESNE ANEANEANKT MPETPTGDSD PQPAPKKMKT SESSTILVVR YRRNVKRTSP EELVNDHARE NRINPDQMEE EEFIEITTER PKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. Epigenetic mechanisms underlying the dynamic expression of cancer-testis genes, PAGE2, -2B and SPANX-B, during mesenchymal-to-epithelial transition PMID: 25229454
  2. Human VCX/Y, SPANX, and CSAG2 gene families together with the murine SPANX gene and the CYPT family may share a common ancestor. PMID: 17342728
  3. Observational study of gene-disease association. (HuGE Navigator) PMID: 20073942
  4. Copy number variation of the entire gene cluster containing all four SPANXA-E genes and with SPANXB, found exclusively in maturing sperm. Average CNV patterns did not differ between fertile and infertile men. PMID: 20073942
  5. correlation between SPAN-Xb gene expression and B-cell immune responses in myeloma and other hematologic malignancies PMID: 12393489
  6. genetic variability of SPANX-B and SPANX-C in a sample of Sicilian male population including patients with melanoma of the skin and controls; Sixteen and 13 genetic classes were detected for SPANX-B and SPANX-C genes, respectively PMID: 18626316
  7. SPANX-B (Xq27 region) gene dosage analysis in Down's syndrome subjects with undescended testes PMID: 19417550
  8. Observational study of gene-disease association. (HuGE Navigator) PMID: 18626316

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Subcellular Location
Cytoplasm. Nucleus. Note=Associated with nuclear craters.
Protein Families
SPAN-X family
Tissue Specificity
Detected in testis and sperm.
Database Links

HGNC: 14329

OMIM: 300669

KEGG: hsa:728695

UniGene: Hs.434105

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