Recombinant Human Synaptic vesicle membrane protein VAT-1 homolog (VAT1)

Code CSB-YP857863HU
MSDS
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Source Yeast
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Code CSB-EP857863HU
MSDS
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Source E.coli
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Code CSB-EP857863HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857863HU
MSDS
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Source Baculovirus
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Code CSB-MP857863HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VAT1
Uniprot No.
Alternative Names
VAT1; FLJ20230; Membrane protein of cholinergic synaptic vesicles; MGC124668; OTTMUSP00000002784; RP23-328K2.5; Synaptic vesicle membrane protein VAT 1 homolog; Synaptic vesicle membrane protein VAT-1 homolog; VAT 1; VAT1; VAT1_HUMAN; VATI; Vesicle amine transport protein 1; Vesicle amine transport protein 1 homolog (T. californica)
Species
Homo sapiens (Human)
Expression Region
2-393
Target Protein Sequence
SDEREVAEA ATGEDASSPP PKTEAASDPQ HPAASEGAAA AAASPPLLRC LVLTGFGGYD KVKLQSRPAA PPAPGPGQLT LRLRACGLNF ADLMARQGLY DRLPPLPVTP GMEGAGVVIA VGEGVSDRKA GDRVMVLNRS GMWQEEVTVP SVQTFLIPEA MTFEEAAALL VNYITAYMVL FDFGNLQPGH SVLVHMAAGG VGMAAVQLCR TVENVTVFGT ASASKHEALK ENGVTHPIDY HTTDYVDEIK KISPKGVDIV MDPLGGSDTA KGYNLLKPMG KVVTYGMANL LTGPKRNLMA LARTWWNQFS VTALQLLQAN RAVCGFHLGY LDGEVELVSG VVARLLALYN QGHIKPHIDS VWPFEKVADA MKQMQEKKNV GKVLLVPGPE KEN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Possesses ATPase activity. Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
Gene References into Functions
  1. We demonstrate that VAT-1 is a novel pathogenic factor in benign prostatic hyperplasia associated with cell proliferation. PMID: 21394740
  2. The role of phospholipase D in the transport of VAT1 to plasma membranes and in phosphatidic acid metabolism in neutrophils is reported. PMID: 20858461
  3. VAT-1 is overexpressed in glioblastomas and functionally involved in glioma cell migration, representing a new component involved in glioma invasion PMID: 19508442
Subcellular Location
Cytoplasm. Mitochondrion outer membrane; Peripheral membrane protein.
Protein Families
Zinc-containing alcohol dehydrogenase family, Quinone oxidoreductase subfamily
Tissue Specificity
Expressed in brain. Also expressed in glioblastoma cells.
Database Links

HGNC: 16919

OMIM: 604631

KEGG: hsa:10493

STRING: 9606.ENSP00000347872

UniGene: Hs.514199

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