Recombinant Human Thyroid hormone receptor beta(THRB)

Code CSB-EP023514HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP023514HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) THRB.

  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP023514HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) THRB.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names THRB
Uniprot No. P10828
Research Area Cardiovascular
Alternative Names Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2; C ERBA 2; C ERBA BETA; c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA2; Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian; generalized resistance to thyroid hormone; GRTH; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; Oncogene ERBA2; PRTH; THB_HUMAN; THR1; THRB 1; THRB 2; thrB; THRB1; THRB2; Thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta 1; Thyroid hormone receptor beta 2; Thyroid hormone receptor beta; Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian)
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-461aa
Target Protein Sequence MTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSERRSTLKNEQSSPHLIQTTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 68.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Citations

Disruptive effects of two organotin pesticides on the thyroid signaling pathway in Xenopus laevis during metamorphosis. Shuying Li, et al,Science of The Total Environment,2019

Applications: Binding assay
Species: Human
Review: Representative sensorgrams for kinetic analysis of hTRβ interactions. The T3 (A) was injected at concentrations of 0.0125, 0.025, 0.05 and 0.1 μM (from bottom to top).
PMID: 31476497

Q&A and Customer Reviews

 Q&A
Q:

Could you please indicate the concentration, purity and tag of cat# CSB-EP023514HU-50?

A:
Thanks for your inquiry. The concentration is determined during the production. The puirity is over 85%. The tag is N-terminal 6xHis-SUMO-tagged.
 Customer Reviews

Target Data

Function Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
Gene References into Functions
  1. These data indicate that m-TRbeta1 can act as a tumor suppressor in hepatocarcinoma and its role was significantly better than that of TRbeta1. PMID: 29679278
  2. Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. PMID: 28864529
  3. Report of a novel mutation T273R in the THRB gene of patients exhibiting signs of the syndrome of resistance to thyroid hormone. Authors propose that the trans-activating function of TRbeta is likely dominantly hindered in these patients. PMID: 28557707
  4. Authors previously shown that Nuclear Receptor Corepressor 1 (NCoR) and the thyroid hormone receptor beta1 (TRbeta) inhibit tumor invasion. Here they show that these molecules repress VEGF-C and VEGF-D gene transcription in breast cancer cells, reducing lymphatic vessel density and sentinel lymph node invasion in tumor xenografts. PMID: 27806339
  5. The results emphasized the importance of TRB1 in the regulation of HSV-1 replication in differentiated environment with neuronal phenotype. PMID: 26843385
  6. In certain contexts, Rb loss enables TRbeta1-dependent suppression of SKP2 as a safeguard against RB1-deficient tumorigenesis. TRbeta2 counteracts TRbeta1, thus disrupting this safeguard and promoting development of RB1-deficient malignancies. PMID: 28972075
  7. A relatively stable genome in retinoblastoma tumor cells is maintained by TRb1 and TRb2-mediated PTTG1 inhibition, counteracting Rb-deficiency-related genomic instability. PMID: 28242412
  8. the actions of R429Q-TRbeta1 in Resistance to Thyroid Hormone (RTH)-Syndrome most likely reflect the reduced hormone affinity observed for this mutant rather than an alteration in target gene repertoire. PMID: 28257829
  9. The present studies uncovered a novel mechanism by which thyroid hormone receptor beta could function as a tumor suppressor through modulation of TNF alpha-IkappaB alpha-NFkappaB pathway. PMID: 27254276
  10. Novel THRB single nucleotide substitution-C to G in codon 340 in resistance to thyroid hormone syndrome. PMID: 28222413
  11. TRbeta suppressed Runx2 transcriptional activities, thus confirming TRbeta regulation of Runx2 at functional thyroid hormone-response elements. Significantly, these findings indicate that a ratio of the tumor-suppressor TRbeta and tumor-promoting Runx2 may reflect tumor aggression and serve as biomarkers in biopsy tissues. The discovery of this TRbeta-Runx2 signaling supports the emerging role of TRbeta as a tumor supp... PMID: 27253998
  12. results revealed that microRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB PMID: 27734462
  13. it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
  14. presents 1 pedigree of thyroid hormone resistance syndrome with heterozygous A317T mutation in THRbeta gene in the proband and his mother, which is the first reported mutation in Chinese and provides a comprehensive review of available literature PMID: 27537566
  15. Molecular cloning and detection of TR beta isoform 4 in pituitary cells. PMID: 26513165
  16. The expression of thyroid receptor beta is linked to fertility status. PMID: 26715425
  17. p.H271D mutation associated with resistance thyroid hormone syndrome PMID: 26041374
  18. diagnosis was confirmed by direct THRB sequencing that revealed 2 novel mutations: the heterozygous p.Ala317Ser in subject 1 and the heterozygous p.Arg438Pro in subject 2 PMID: 25738994
  19. Down-regulation of THRbeta correlates with the reduction of all markers of differentiation and is associated with overexpression of some miRNAs supposed to play a role in thyroid tumorigenesis. PMID: 26003825
  20. propose that the mutated C-terminal region of TRbeta1 could function as an "onco-domain" PMID: 25924236
  21. cases suggested that certain RTHbeta mutants such as p.R316C might show very mild syndrome of inappropriate secretion of thyrotropin in spite of having apparent peripheral resistance to thyroid hormone PMID: 25502991
  22. Loss of heterozygosity in THRB and its proximal microsatellite markers may play a role in tumorigenesis and development in invasive breast cancer. PMID: 26350179
  23. Results showed that triple negative breast cancer patients expressed low level of THRB which was associated with poor outcome and enhanced resistance to both docetaxel and doxorubicin treatment. PMID: 25820519
  24. It is demonstrated in this article that the A234T mutation in the THR-beta gene can cause the thyroid hormone resistance syndrome. PMID: 26273722
  25. Its promoter methylation may be involved in the development of gastric cancer. PMID: 25302749
  26. data indicated altered mineral metabolism in adults and children with resistance to thyroid hormone due to mutations in the THRB gene PMID: 25063548
  27. data demonstrate that TR sumoylation is required for activation of the Wnt canonical signaling pathway during preadipocyte proliferation and enhances the PPARgamma signaling that promotes differentiation PMID: 25572392
  28. down-regulated in hepatocellular carcinoma and precancerous peritumoral tissue PMID: 25156012
  29. miR-424 or miR-503 mediate anti-proliferative and anti-invasive actions of thyroid hormone receptor beta PMID: 24796297
  30. CpG methylation is not the major mechanism contributing to decreased THRB expression in ccRCC PMID: 24849932
  31. Coordinated regulation of transcription and alternative splicing by the thyroid hormone receptor.Thyroid hormone receptor stimulates target gene transcription by binding to the thyroid hormone-response element in the presence of triiodothyronine. PMID: 25019984
  32. The clinical and biochemical characteristics of our samples of Mediterranean populations with thyroid hormone resistance were similar to those previously described. Interestingly, in our populations we identified four novel mutations in the TRbeta gene PMID: 24722129
  33. TRb expression in FTC cells decreased cancer cell proliferation, impeded tumor cell migration, and inhibited tumor growth in vivo through downregulation of the AKT-mTOR-p70 S6P signaling pathway and suppression of VEGF expression PMID: 23731250
  34. T3 throid hormone enhanced the recruitment of the TRbeta1/Oct-1 complex on Octamer-transcription factor-1 site within cyclin D1 promoter. PMID: 24121026
  35. ONL001656673 PMID: 20082849
  36. A heterozygous mutation at codon 350 located in exon 9 of the THRB gene was detected in all the affected members of the family. It is important to consider thyroid hormone levels in association with TSH levels to prevent inappropriate treatmen PMID: 24217081
  37. Using several human ROP enhancer/promoter-luciferase reporter constructs, the study found that thyroid hormone receptor beta 2 increased luciferase activities through the 5'-UTR and intron 3-4 region. PMID: 24058409
  38. Alzheimer's disease brain tissues with elevated neuroserpin protein also showed increased expression of THRbeta1 and HuD PMID: 24036060
  39. Results showthat thyroid hormone receptor beta1 (TRbeta1) mRNA expression was significantly reduced in gastric cancer specimens and the methylation of promoter of TRbeta1 gene in gastric cancer tissues was significantly higher than in adjacent normal tissues. PMID: 24434154
  40. SIRT1 stimulates THRB1 activity in a manner that is independent of PGC-1alpha but requires SIRT1 deacetylase activity. PMID: 23922917
  41. Adenovirus E1A 55R functions as a strong co-activator of TR-dependent transcription. PMID: 24136366
  42. A combination of isoform-specific recruitment and tissue-specific expression of these newly identified coregulator candidates serves to customize TR function for different biological purposes in different cell types. PMID: 23558175
  43. Data indicate that 3,5-diiodothyronine T2 activates the thyroid hormone receptor beta1 (TRbeta1). PMID: 23736295
  44. we report a novel mutation in the THRb gene [c.986C>T (p.Thr329Ile)] in a woman and her daughter, who was also a carrier of the same mutation. PMID: 23195042
  45. TR-beta and LXR-alpha competitively up-regulate the human Seladin-1 promoter, sharing the same response element, site A. PMID: 23416078
  46. One SNP 5' of the thyroid hormone receptor-beta gene was associated with BDR in the childhood population and two adult populations (P-value=0.0012- PMID: 22212731
  47. a novel inverse recruitment mechanism in which liganded TRbeta recruits corepressors to inhibit PLA2g2a expression. PMID: 23629656
  48. THRB intronic SNPs can provide useful information on chemoradiotherapy-related severe myelotoxicity in patients with esophageal squamous cell carcinoma PMID: 23136537
  49. We document a congenital disorder of cone function characterized by severely reduced L- and M-cone responses and increased S-cone responses caused by deleterious mutations in the THRbeta2 gene in thyroid resistant patients. PMID: 22551329
  50. THRB mRNA expression in DTC was 90% lower than in normal controls, and this decrease was associated with a higher tumor/lymph node staging. PMID: 23183175
  51. TRbeta1 binds PGC-1alpha in a second ligand and LxxLL motif independent mode and show this interaction requires the TRbeta1 N-terminal domain and the PGC-1alpha N-terminal activation domain at amino acids 1-130. PMID: 22974658
  52. Pathogenic mutations in the thyroid hormone receptor beta gene is associated with thyroid hormone resistance. PMID: 21795843
  53. SNPs in intron control region (ICR) of THRB gene in resistance to thyroid hormone(RTH)syndrome; in index case of PRTH a common SNP in ICR stimulates overactivation of TRbeta2 promoter in pituitary cells; it is linked in cis with known R338W coding mutation PMID: 21871106
  54. report of three new subjects, from two families, in whom resistance to thyroid hormone was associated with homozygous mutations in the THRB gene; report strengthens the concept that the mutated TRbeta interferes with the function of the TRalpha1 in humans PMID: 22319036
  55. Genes in the erythroid differentiation and cell cycle regulation pathways influence interindividual variation in RBC indices. Our results provide insights into the molecular basis underlying variation in RBC traits. PMID: 22560525
  56. Firefly luciferase cDNA sequence mediates the phorbol 12-O-tetradecanoate-13-acetate-induced transcriptional activity, which is inhibited by thyroid hormone/thyroid hormone receptor. PMID: 22253701
  57. The TR/DKK4/Wnt/beta-catenin cascade influences the proliferation and migration of hepatoma cells during the metastasis process and support a tumor suppressor role of the thyroid hormone receptor. PMID: 21994129
  58. THRB mutations were found in 3 cases of familial thyroid hormone resistance: R243Q, R320C, R429Q. They had decreased response to T3. R243Q & R320C had a strong dominant negative effect. PMID: 21703645
  59. a novel missense mutation in exon 9 of the TRbeta gene was identified in a boy with resistance to thyroid hormone, changing codon 317 from GCT to GAT, 1235C>A or A317D the same mutation was also identified in his mother PMID: 21740842
  60. Although residues at position 280 do not interact with coactivators or with the ligand, the authors show that its mutations can selectively block coactivator and corepressor binding, and affect hormone binding affinity differently. PMID: 21530542
  61. charge clusters allow wild-type thyroid hormone receptor beta 2 to assume a conformation compatible with its mode of multiple contact coactivator recruitment PMID: 21622532
  62. TRbeta dimer/heterodimer interactions influence ligand (125I-T3) association/dissociation kinetic; studies include mutant/recombinant forms of TRbeta, ligand-binding domain of TRbeta, corepressor peptide binding, and heterodimers with RXRalpha. PMID: 21508093
  63. TRbeta1 degradation is mediated by the proteasome pathway. kinetics of TRbeta1 degradation is atypical due to the co-existence of more than one TRbeta1 population, located in different cellular compartments and having different stability profiles. PMID: 21323586
  64. microRNAs up-regulated in papillary thyroid carcinoma tumors directly inhibit the expression of THRB, an important tumor suppressor gene PMID: 21159845
  65. RTH is a rare autosomal-dominant inherited disorder. The majority of cases are caused by a mutation in the thyroid hormone receptor beta gene [3] leading to impaired responsiveness of the tissues to thyroid hormones. PMID: 21110296
  66. recombinant RXR-alpha could effectively bind TRbeta1 to form a heterodimer PMID: 21402532
  67. An important functional role of endogenous corepressors was shown in the suppression of ras-mediated transformation and tumorigenesis by thyroid hormone receptor beta1. PMID: 20956942
  68. T3 weakens TRb1 binding to a negative regulatory element in the TSHbeta promoter. PMID: 20838640
  69. THRB is regulated by 7 out of 8 microRNA genes upregulated in papillary thyroid cancer, including miR-21, miR-146a, miR-221, and miR-181a PMID: 21159845
  70. Data show that reduced TRbeta1 expression and tissue hypothyroidism in ccRCC tumors is likely to be involved in the process of carcinogenesis or in maintaining a proliferative advantage to malignant cells. PMID: 20691260
  71. Thyroid hormone receptors THRbeta and THRalpha1 are down-regulated in skeletal muscle from patients with non-thyroidal illness syndrome secondary to non-septic shock. PMID: 20736347
  72. epigenetic inactivation of TRbeta1 through aberrant methylation in lung cancer PMID: 20155399
  73. A novel isoform, TRbeta4, may modulate T3 action as an endogenous antagonist in the tissue or cellular context, like TRalpha2. PMID: 20470753
  74. Thyroid transcription factor 1 expression confers a better prognosis in patients with ovarian cancer. PMID: 20004952
  75. Data show that TH treatment promoted rapid and sustained hCCR4 recruitment to the TH-responsive deiodinase 1 promoter and TR co-localizes with hCCR4 in the nucleus and interacts with hCCR4 in 2-hybrid and pull-down assays. PMID: 19903885
  76. TR surfaces and conformations required to bind nuclear receptor corepressor PMID: 11818500
  77. neurodevelopmental functions of thyroid hormone signaling PMID: 11861164
  78. affected receptor amino acid sequences.lost their trans-activation function and exhibited dominant negative activity. PMID: 11889175
  79. genetic analysis revealed a novel heterozygous missense mutation at codon 334 of thyroid hormone receptor beta1 in a family with resistance to thyroid hormone PMID: 11929097
  80. a point mutation, not anticipated to occur in resistance to thyroid hormone, causes variable phenotypes. PMID: 12006711
  81. Thrb(PV)/Thrb(PV) mice have severe hearing impairment that is already present at 3 weeks of age. This hearing loss is associated with disruption of postnatal morphogenesis of the tectorial membrane and organ of Corti. PMID: 12382103
  82. x-ray crystal structures of two hTRbeta ligand-binding domains, Ala 317 Thr and Arg 316 His PMID: 12554782
  83. expression of functionally impaired mutants of THRB1 in papillary carcinomas is rare PMID: 12843201
  84. The crystal structure of human thyroid hormone receptor beta at 2.8-A resolution with GC-24 bound explains its agonist activity and unique isoform specificity. PMID: 14673100
  85. Except for cardiac hypertrophy, the presence of a germline TR-beta mutation had surprisingly little effect on cardiac function. PMID: 14684607
  86. thyroid hormone receptor-beta cell cycle-dependent expression regulates variable hormone sensitivity PMID: 14767065
  87. partial dissociation of TR/retinoid X receptor heterodimer complex from the TRE is involved in the suppression of transcription induced by polychlorinated biphenyls PMID: 14985366
  88. novel point mutation, a heterozygous transition c.1031G>C in exon 9 theoretically substituting Gly344Ala results in bone development retardation PMID: 15080770
  89. developmental and tissue-specific expression of human thyroid hormone receptor beta1 5'-UTR mRNAs may regulate T3-responsiveness in target tissues by modulating TRbeta protein translation PMID: 15105435
  90. Most patients with resistance to thyroid hormone carry a mutation in this gene. PMID: 15186611
  91. unliganded TRbeta1 suppresses promoter activity driven by LXRalpha and its ligand, whereas transactivation by T3-bound TRbeta1 is not affected by LXRalpha in the presence or absence of oxysterols PMID: 15319359
  92. two novel TRbeta mutations occurring in the same nucleotide PMID: 15598685
  93. results suggested that T3 upregulates cellular proliferation on LNCaP cells but not other prostatic carcinoma cells and PZ-HPV-7 and CA-HPV-10 cells express the novel TRbeta1, which locates at cell nuclear membrane PMID: 15867011
  94. a small subset of TRbeta mutations that arise in resistance to thyroid hormone syndrome inhibit both T3 binding and formation of TRbeta homodimers on thyroid hormone response elements PMID: 15886199
  95. Modulation of hepatic TRbeta1, a key regulator of gene expression involved in lipid metabolism by soy proteins may be a novel mechanism by which soy components lower blood lipid level and exert their hypocholesterolemic actions. PMID: 15987841
  96. Pituitary and peripheral tissue responses to graded doses of liothyronine in 5 affected members of a family with thyroid hormone resistance due to the common TRbeta mutation P453T are reported. PMID: 16099238
  97. Association of TRbeta1 expression with growth pattern and the presence of K-ras mutations suggest that abnormalities in thyroid hormone signalling involving TRbeta1 play a role in the development of some types of colorectal adenocarcinomas PMID: 16231318
  98. The E333D TRbeta mutation is responsible for the resistance to thyroid hormone syndrome in a case report. PMID: 17177139
  99. apo THRBs form tetramers in solution which dissociate into dimers upon thyroid hormone T3 binding PMID: 17260956
  100. Mediates Akt activation by T3 in pancreatic beta cells. PMID: 17293442

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Involvement in disease Generalized thyroid hormone resistance (GTHR); Generalized thyroid hormone resistance autosomal recessive (GTHRAR); Selective pituitary thyroid hormone resistance (PRTH)
Subcellular Location Nucleus
Protein Families Nuclear hormone receptor family, NR1 subfamily
Database Links

HGNC: 11799

OMIM: 145650

KEGG: hsa:7068

STRING: 9606.ENSP00000348827

UniGene: Hs.187861

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