Name: Recombinant Human Thyroid hormone receptor beta (THRB)
Brand: CUSABIO
SKU: CSB-EP023514HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

THRB

Uniprot No.

P10828

Research Area

Cardiovascular

Alternative Names

Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2; C ERBA 2; C ERBA BETA; c-erbA-2; c-erbA-beta; ERBA 2; ERBA BETA; ERBA2; Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian; generalized resistance to thyroid hormone; GRTH; MGC126109; MGC126110; NR1A2; Nuclear receptor subfamily 1 group A member 2; Oncogene ERBA2; PRTH; THB_HUMAN; THR1; THRB 1; THRB 2; thrB; THRB1; THRB2; Thyroid hormone nuclear receptor beta variant 1; Thyroid hormone receptor beta 1; Thyroid hormone receptor beta 2; Thyroid hormone receptor beta; Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-461aa

Target Protein Sequence

MTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSERRSTLKNEQSSPHLIQTTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVLDDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWKQKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCEDQIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSLSSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

68.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-461 form the expressed segment for recombinant Human THRB. This THRB protein is theoretically predicted to have a molecular weight of 68.8 kDa. This protein is generated in a e.coli-based system. The THRB coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant THRB protein in following stages of expression and purification.

Thyroid hormone receptor beta (THRB) is a nuclear receptor protein that plays a crucial role in mediating the effects of thyroid hormones on gene expression and overall metabolism. As a member of the thyroid hormone receptor family, THRB functions as a transcription factor that binds to thyroid hormone response elements (TREs) in the promoter regions of target genes. Upon binding to triiodothyronine (T3), the biologically active form of thyroid hormone, THRB undergoes conformational changes that enable its interaction with co-regulatory proteins, leading to the regulation of gene transcription. THRB is primarily expressed in tissues such as the brain, liver, and heart, where it influences processes like growth, development, and energy homeostasis. Dysregulation of THRB is associated with thyroid hormone resistance syndromes and various metabolic disorders. In research, THRB is studied to elucidate its molecular mechanisms, understand its role in physiology, and explore its potential as a therapeutic target for thyroid-related and metabolic diseases.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Binding assay

Review: Representative sensorgrams for kinetic analysis of hTRβ interactions. The T3 (A) was injected at concentrations of 0.0125, 0.025, 0.05 and 0.1 μM (from bottom to top).

By Anonymous

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■ Q&A

Could you please indicate the concentration, purity and tag of cat# CSB-EP023514HU-50?

Thanks for your inquiry. The concentration is determined during the production. The puirity is over 85%. The tag is N-terminal 6xHis-SUMO-tagged.

Target Background

Function

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

Gene References into Functions

These data indicate that m-TRbeta1 can act as a tumor suppressor in hepatocarcinoma and its role was significantly better than that of TRbeta1. PMID: 29679278
Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation. PMID: 28864529
Report of a novel mutation T273R in the THRB gene of patients exhibiting signs of the syndrome of resistance to thyroid hormone. Authors propose that the trans-activating function of TRbeta is likely dominantly hindered in these patients. PMID: 28557707
Authors previously shown that Nuclear Receptor Corepressor 1 (NCoR) and the thyroid hormone receptor beta1 (TRbeta) inhibit tumor invasion. Here they show that these molecules repress VEGF-C and VEGF-D gene transcription in breast cancer cells, reducing lymphatic vessel density and sentinel lymph node invasion in tumor xenografts. PMID: 27806339
The results emphasized the importance of TRB1 in the regulation of HSV-1 replication in differentiated environment with neuronal phenotype. PMID: 26843385
In certain contexts, Rb loss enables TRbeta1-dependent suppression of SKP2 as a safeguard against RB1-deficient tumorigenesis. TRbeta2 counteracts TRbeta1, thus disrupting this safeguard and promoting development of RB1-deficient malignancies. PMID: 28972075
A relatively stable genome in retinoblastoma tumor cells is maintained by TRb1 and TRb2-mediated PTTG1 inhibition, counteracting Rb-deficiency-related genomic instability. PMID: 28242412
the actions of R429Q-TRbeta1 in Resistance to Thyroid Hormone (RTH)-Syndrome most likely reflect the reduced hormone affinity observed for this mutant rather than an alteration in target gene repertoire. PMID: 28257829
The present studies uncovered a novel mechanism by which thyroid hormone receptor beta could function as a tumor suppressor through modulation of TNF alpha-IkappaB alpha-NFkappaB pathway. PMID: 27254276
Novel THRB single nucleotide substitution-C to G in codon 340 in resistance to thyroid hormone syndrome. PMID: 28222413
TRbeta suppressed Runx2 transcriptional activities, thus confirming TRbeta regulation of Runx2 at functional thyroid hormone-response elements. Significantly, these findings indicate that a ratio of the tumor-suppressor TRbeta and tumor-promoting Runx2 may reflect tumor aggression and serve as biomarkers in biopsy tissues. The discovery of this TRbeta-Runx2 signaling supports the emerging role of TRbeta as a tumor supp... PMID: 27253998
results revealed that microRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB PMID: 27734462
it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
presents 1 pedigree of thyroid hormone resistance syndrome with heterozygous A317T mutation in THRbeta gene in the proband and his mother, which is the first reported mutation in Chinese and provides a comprehensive review of available literature PMID: 27537566
Molecular cloning and detection of TR beta isoform 4 in pituitary cells. PMID: 26513165
The expression of thyroid receptor beta is linked to fertility status. PMID: 26715425
p.H271D mutation associated with resistance thyroid hormone syndrome PMID: 26041374
diagnosis was confirmed by direct THRB sequencing that revealed 2 novel mutations: the heterozygous p.Ala317Ser in subject 1 and the heterozygous p.Arg438Pro in subject 2 PMID: 25738994
Down-regulation of THRbeta correlates with the reduction of all markers of differentiation and is associated with overexpression of some miRNAs supposed to play a role in thyroid tumorigenesis. PMID: 26003825
propose that the mutated C-terminal region of TRbeta1 could function as an "onco-domain" PMID: 25924236
cases suggested that certain RTHbeta mutants such as p.R316C might show very mild syndrome of inappropriate secretion of thyrotropin in spite of having apparent peripheral resistance to thyroid hormone PMID: 25502991
Loss of heterozygosity in THRB and its proximal microsatellite markers may play a role in tumorigenesis and development in invasive breast cancer. PMID: 26350179
Results showed that triple negative breast cancer patients expressed low level of THRB which was associated with poor outcome and enhanced resistance to both docetaxel and doxorubicin treatment. PMID: 25820519
It is demonstrated in this article that the A234T mutation in the THR-beta gene can cause the thyroid hormone resistance syndrome. PMID: 26273722
Its promoter methylation may be involved in the development of gastric cancer. PMID: 25302749
data indicated altered mineral metabolism in adults and children with resistance to thyroid hormone due to mutations in the THRB gene PMID: 25063548
data demonstrate that TR sumoylation is required for activation of the Wnt canonical signaling pathway during preadipocyte proliferation and enhances the PPARgamma signaling that promotes differentiation PMID: 25572392
down-regulated in hepatocellular carcinoma and precancerous peritumoral tissue PMID: 25156012
miR-424 or miR-503 mediate anti-proliferative and anti-invasive actions of thyroid hormone receptor beta PMID: 24796297
CpG methylation is not the major mechanism contributing to decreased THRB expression in ccRCC PMID: 24849932
Coordinated regulation of transcription and alternative splicing by the thyroid hormone receptor.Thyroid hormone receptor stimulates target gene transcription by binding to the thyroid hormone-response element in the presence of triiodothyronine. PMID: 25019984
The clinical and biochemical characteristics of our samples of Mediterranean populations with thyroid hormone resistance were similar to those previously described. Interestingly, in our populations we identified four novel mutations in the TRbeta gene PMID: 24722129
TRb expression in FTC cells decreased cancer cell proliferation, impeded tumor cell migration, and inhibited tumor growth in vivo through downregulation of the AKT-mTOR-p70 S6P signaling pathway and suppression of VEGF expression PMID: 23731250
T3 throid hormone enhanced the recruitment of the TRbeta1/Oct-1 complex on Octamer-transcription factor-1 site within cyclin D1 promoter. PMID: 24121026
ONL001656673 PMID: 20082849
A heterozygous mutation at codon 350 located in exon 9 of the THRB gene was detected in all the affected members of the family. It is important to consider thyroid hormone levels in association with TSH levels to prevent inappropriate treatmen PMID: 24217081
Using several human ROP enhancer/promoter-luciferase reporter constructs, the study found that thyroid hormone receptor beta 2 increased luciferase activities through the 5'-UTR and intron 3-4 region. PMID: 24058409
Alzheimer's disease brain tissues with elevated neuroserpin protein also showed increased expression of THRbeta1 and HuD PMID: 24036060
Results showthat thyroid hormone receptor beta1 (TRbeta1) mRNA expression was significantly reduced in gastric cancer specimens and the methylation of promoter of TRbeta1 gene in gastric cancer tissues was significantly higher than in adjacent normal tissues. PMID: 24434154
SIRT1 stimulates THRB1 activity in a manner that is independent of PGC-1alpha but requires SIRT1 deacetylase activity. PMID: 23922917
Adenovirus E1A 55R functions as a strong co-activator of TR-dependent transcription. PMID: 24136366
A combination of isoform-specific recruitment and tissue-specific expression of these newly identified coregulator candidates serves to customize TR function for different biological purposes in different cell types. PMID: 23558175
Data indicate that 3,5-diiodothyronine T2 activates the thyroid hormone receptor beta1 (TRbeta1). PMID: 23736295
we report a novel mutation in the THRb gene [c.986C>T (p.Thr329Ile)] in a woman and her daughter, who was also a carrier of the same mutation. PMID: 23195042
TR-beta and LXR-alpha competitively up-regulate the human Seladin-1 promoter, sharing the same response element, site A. PMID: 23416078
One SNP 5' of the thyroid hormone receptor-beta gene was associated with BDR in the childhood population and two adult populations (P-value=0.0012- PMID: 22212731
a novel inverse recruitment mechanism in which liganded TRbeta recruits corepressors to inhibit PLA2g2a expression. PMID: 23629656
THRB intronic SNPs can provide useful information on chemoradiotherapy-related severe myelotoxicity in patients with esophageal squamous cell carcinoma PMID: 23136537
We document a congenital disorder of cone function characterized by severely reduced L- and M-cone responses and increased S-cone responses caused by deleterious mutations in the THRbeta2 gene in thyroid resistant patients. PMID: 22551329
THRB mRNA expression in DTC was 90% lower than in normal controls, and this decrease was associated with a higher tumor/lymph node staging. PMID: 23183175

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Involvement in disease

Generalized thyroid hormone resistance (GTHR); Generalized thyroid hormone resistance autosomal recessive (GTHRAR); Selective pituitary thyroid hormone resistance (PRTH)

Subcellular Location

Nucleus.

Protein Families

Nuclear hormone receptor family, NR1 subfamily

Database Links

HGNC: 11799

OMIM: 145650

KEGG: hsa:7068

STRING: 9606.ENSP00000348827

UniGene: Hs.187861

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Code	CSB-EP023514HU
MSDS	MSDS
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP023514HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) THRB. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP023514HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) THRB.
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Recombinant Human Thyroid hormone receptor beta (THRB)

Product Details

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Target Background

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