Recombinant Human Zinc finger protein 580 (ZNF580)

Code CSB-YP026862HU
MSDS
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Source Yeast
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Code CSB-EP026862HU
MSDS
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Source E.coli
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Code CSB-EP026862HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026862HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP026862HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ZNF580
Uniprot No.
Alternative Names
LDL induced EC protein; LDL-induced EC protein; Zinc finger protein 580; ZN580_HUMAN; Znf580
Species
Homo sapiens (Human)
Expression Region
1-172
Target Protein Sequence
MLLLPPRPPH PRSSSPEAMD PPPPKAPPFP KAEGPSSTPS SAAGPRPPRL GRHLLIDANG VPYTYTVQLE EEPRGPPQRE APPGEPGPRK GYSCPECARV FASPLRLQSH RVSHSDLKPF TCGACGKAFK RSSHLSRHRA THRARAGPPH TCPLCPRRFQ DAAELAQHVR LH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the regulation of endothelial cell proliferation and migration. Mediates H(2)O(2)-induced leukocyte chemotaxis by elevating interleukin-8 production and may play a role in inflammation. May be involved in transcriptional regulation.
Gene References into Functions
  1. These results suggest that ZNF580 mediates eNOS expression and endothelial cell migration/proliferation via the TGF-b1/ALK5/Smad2 pathway, and thus plays a crucial role in vascular endothelial cells. PMID: 24771066
  2. H2O2 upregulates the expression of ZNF580 via the NF-kappaB signaling pathway, and overexpression of ZNF580 plays a critical role in augmenting the release of pro-inflammatory cytokine IL-8. PMID: 21830064
  3. ZNF580 is a binding partner of Smad2 and is involved in the signal transduction of the transforming growth factor-beta signalling pathway. PMID: 21599657
  4. ZNF580 is a novel factor in the lipoprotein-dependent regulation of endothelial IL-8 expression and monocyte arrest. PMID: 21310414
  5. These results elucidate the important role that ZNF580 plays in the process of migration and proliferation of endothelial cells, which provides a foundation for a novel approach to regulate angiogenesis. PMID: 20382120

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Subcellular Location
Nucleus. Note=Colocalized with SMAD2 in the nucleus.
Tissue Specificity
Expressed in endothelial cells.
Database Links

HGNC: 29473

OMIM: 617888

KEGG: hsa:51157

STRING: 9606.ENSP00000320050

UniGene: Hs.631551

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