Recombinant Hydroxymycolate synthase MmaA4 (mmaA4)

Code CSB-YP745133MVZ
MSDS
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Source Yeast
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Code CSB-EP745133MVZ
MSDS
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Source E.coli
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Code CSB-EP745133MVZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP745133MVZ
MSDS
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Source Baculovirus
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Code CSB-MP745133MVZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mmaA4
Uniprot No.
Alternative Names
mmaA4; hma; mma4; Rv0642c; Hydroxymycolate synthase MmaA4; EC 2.1.1.-; Mycolic acid methyltransferase; MA-MT; S-adenosylmethionine-dependent methyltransferase; AdoMet-MT; SAM-MT
Species
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Expression Region
2-301
Target Protein Sequence
TRMAEKPIS PTKTRTRFED IQAHYDVSDD FFALFQDPTR TYSCAYFEPP ELTLEEAQYA KVDLNLDKLD LKPGMTLLDI GCGWGTTMRR AVERFDVNVI GLTLSKNQHA RCEQVLASID TNRSRQVLLQ GWEDFAEPVD RIVSIEAFEH FGHENYDDFF KRCFNIMPAD GRMTVQSSVS YHPYEMAARG KKLSFETARF IKFIVTEIFP GGRLPSTEMM VEHGEKAGFT VPEPLSLRPH YIKTLRIWGD TLQSNKDKAI EVTSEEVYNR YMKYLRGCEH YFTDEMLDCS LVTYLKPGAA A
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the biosynthesis of hydroxymycolate, a common precursor of oxygenated mycolic acids (methoxy-mycolate and keto-mycolate). Probably transfers a methyl group from the S-adenosylmethionine (SAM) cofactor and, subsequently or simultaneously, a water molecule onto the double bound of ethylene substrates, leading to the formation of the hydroxylated product at the distal position. Involved in the activation of the antitubercular drug thiacetazone (TAC).
Gene References into Functions
  1. Mutants in which mmaA4 (hma) was inactivated stimulated macrophages to produce significantly more IL-12p40 and TNF-alpha than wild-type M. tuberculosis and were attenuated for virulence PMID: 18535659
  2. mmaA4 mutants were highly resistant to thiacetazone and lacked keto-mycolic acids in the cell wall. PMID: 19183278
Protein Families
CFA/CMAS family
Database Links

KEGG: mtu:Rv0642c

STRING: 83332.Rv0642c

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