Recombinant Methanocaldococcus jannaschii Probable threonylcarbamoyladenosine tRNA methylthiotransferase (MJ0867)

Code CSB-YP710368MRU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP710368MRU
MSDS
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Source E.coli
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Code CSB-EP710368MRU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP710368MRU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP710368MRU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MJ0867
Uniprot No.
Alternative Names
MJ0867Probable threonylcarbamoyladenosine tRNA methylthiotransferase; EC 2.8.4.5; tRNA-t(6)A37 methylthiotransferase
Species
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Expression Region
1-427
Target Protein Sequence
MWLYYLQVVM DMRVYVEGYG CVLNTADTEI IKNSLKKHGF EVVNNLEEAD IAIINTCVVR LETENRMIYR INELKNLGKE VVVAGCLPKA LKNKVKGFLH IYPREAHKAG EILKNYVEKH YRMPYIEEDI NKTLYKKLDY LKPSLITPLP ICEGCIGNCS YCIVKIARGG LISYPREKIV NKAKELINKG AKCLLITAQD TACYGFDIGD NLANLLNELT QIKGEFIMRV GMMHAKNAEL ILDELIEVYQ NEKVGKFLHL PLQSGDDEIL KRMKRGYTVD EFKDIVNEFR RKIKNLCFTT DIIVGFPGET EEQFQNTLEV LRELKPDYIH GAKYSQRKGT EAAKMKQIDT KIRKRRSEIL DKLRRELSYL NNKKYIGKAM KVLVLDEGKG YTDNFKVVKF EGGEVGEFRK VKITDAKTFG LKGELIL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
Protein Families
Methylthiotransferase family, CDKAL1 subfamily
Database Links

KEGG: mja:MJ_0867

STRING: 243232.MJ_0867

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