Recombinant Methylocella silvestris Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Product Type Recombinant Protein
Uniprot No. B8EPC0
Species Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)
Gene Names gatB
Protein Names Recommended name: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B Short name= Asp/Glu-ADT subunit B EC= 6.3.5.-
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Protein Families GatB/GatE family, GatB subfamily
Database Links

KEGG: msl:Msil_0737

STRING: 395965.Msil_0737

Product Details for Different Expression Systems

Product Name Recombinant Methylocella silvestris Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B,Yeast
Code CSB-YP490508MTF
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTRAKPSKL IKGATGDFEI VIGMEIHAQV TSRSKLFSGA STEFGGEPNA HVSLVDAAMP GMLPVINQEC VAQAVRTGLG LEAQINLRSV FDRKNYFYPD LPQGYQISQY KSPIVGEGVV AVDVSPTEQI KVGIERLHLE QDAGKSLHDQ SATESLVDLN RSGVALMEIV SKPDMRSSDE ARAYVTKLRT ILRYIGSCDG NMEQGSLRAD VNVSVRRPGE PLGTRCEIKN VNSIRFIGQA IEVEARRQIG VIEDGGVVQQ ETRLFDPGRG ETRSMRSKEE AHDYRYFPDP DLLPLEFDQA YVDGLKAGLP ELPDAKKERF IAEYGLPAYD AGVLVADKET ADYYEAAVRH GGAKRDPKLV ANWLTGDVAA YANSVGLSVA QTHIKPGQIA SLVDLIADGT ISGKIAKDVL SILIAEEKDA DPKDIVEARG LRQVTDTGAI AAAVDAIIKA NPDKVAQAQA KPSMLGWFVG QVMKQTGGKA NPQAVNDELK AKLGI
Expression Region 1-495
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Methylocella silvestris Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B,E.coli
Code CSB-EP490508MTF
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTRAKPSKL IKGATGDFEI VIGMEIHAQV TSRSKLFSGA STEFGGEPNA HVSLVDAAMP GMLPVINQEC VAQAVRTGLG LEAQINLRSV FDRKNYFYPD LPQGYQISQY KSPIVGEGVV AVDVSPTEQI KVGIERLHLE QDAGKSLHDQ SATESLVDLN RSGVALMEIV SKPDMRSSDE ARAYVTKLRT ILRYIGSCDG NMEQGSLRAD VNVSVRRPGE PLGTRCEIKN VNSIRFIGQA IEVEARRQIG VIEDGGVVQQ ETRLFDPGRG ETRSMRSKEE AHDYRYFPDP DLLPLEFDQA YVDGLKAGLP ELPDAKKERF IAEYGLPAYD AGVLVADKET ADYYEAAVRH GGAKRDPKLV ANWLTGDVAA YANSVGLSVA QTHIKPGQIA SLVDLIADGT ISGKIAKDVL SILIAEEKDA DPKDIVEARG LRQVTDTGAI AAAVDAIIKA NPDKVAQAQA KPSMLGWFVG QVMKQTGGKA NPQAVNDELK AKLGI
Expression Region 1-495
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Methylocella silvestris Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B,Baculovirus
Code CSB-BP490508MTF
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTRAKPSKL IKGATGDFEI VIGMEIHAQV TSRSKLFSGA STEFGGEPNA HVSLVDAAMP GMLPVINQEC VAQAVRTGLG LEAQINLRSV FDRKNYFYPD LPQGYQISQY KSPIVGEGVV AVDVSPTEQI KVGIERLHLE QDAGKSLHDQ SATESLVDLN RSGVALMEIV SKPDMRSSDE ARAYVTKLRT ILRYIGSCDG NMEQGSLRAD VNVSVRRPGE PLGTRCEIKN VNSIRFIGQA IEVEARRQIG VIEDGGVVQQ ETRLFDPGRG ETRSMRSKEE AHDYRYFPDP DLLPLEFDQA YVDGLKAGLP ELPDAKKERF IAEYGLPAYD AGVLVADKET ADYYEAAVRH GGAKRDPKLV ANWLTGDVAA YANSVGLSVA QTHIKPGQIA SLVDLIADGT ISGKIAKDVL SILIAEEKDA DPKDIVEARG LRQVTDTGAI AAAVDAIIKA NPDKVAQAQA KPSMLGWFVG QVMKQTGGKA NPQAVNDELK AKLGI
Expression Region 1-495
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Methylocella silvestris Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B,Mammalian cell
Code CSB-MP490508MTF
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTRAKPSKL IKGATGDFEI VIGMEIHAQV TSRSKLFSGA STEFGGEPNA HVSLVDAAMP GMLPVINQEC VAQAVRTGLG LEAQINLRSV FDRKNYFYPD LPQGYQISQY KSPIVGEGVV AVDVSPTEQI KVGIERLHLE QDAGKSLHDQ SATESLVDLN RSGVALMEIV SKPDMRSSDE ARAYVTKLRT ILRYIGSCDG NMEQGSLRAD VNVSVRRPGE PLGTRCEIKN VNSIRFIGQA IEVEARRQIG VIEDGGVVQQ ETRLFDPGRG ETRSMRSKEE AHDYRYFPDP DLLPLEFDQA YVDGLKAGLP ELPDAKKERF IAEYGLPAYD AGVLVADKET ADYYEAAVRH GGAKRDPKLV ANWLTGDVAA YANSVGLSVA QTHIKPGQIA SLVDLIADGT ISGKIAKDVL SILIAEEKDA DPKDIVEARG LRQVTDTGAI AAAVDAIIKA NPDKVAQAQA KPSMLGWFVG QVMKQTGGKA NPQAVNDELK AKLGI
Expression Region 1-495
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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